From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0821 [new locus tag: NWMN_RS04640 ]
  • pan locus tag?: SAUPAN003052000
  • symbol: mnhB
  • pan gene symbol?: mnhB1
  • synonym:
  • product: putative monovalent cation/H+ antiporter subunit B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0821 [new locus tag: NWMN_RS04640 ]
  • symbol: mnhB
  • product: putative monovalent cation/H+ antiporter subunit B
  • replicon: chromosome
  • strand: -
  • coordinates: 907915..908343
  • length: 429
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAATAGACAACAAAATGATCTAATTTTGCAATTTGCTGCAGTTATTATCTTTTTCATG
    GTAATGGTCTTTGGTTTTTCACTTTTCTTAGCAGGACATTATACACCTGGTGGCGGTTTC
    GTCGGCGGATTATTATTCGCAAGTTCACTTGTTATCATTACGATTGCATTTGATATAGAA
    ACAATGCGTAAAATTTTCCCATTAGATTTTAAAATCTTGATAGGTATCGGATTAGTTTTT
    TGTATTGCGACGCCTATCGCAAGTTGGTTTTTAGGCAAAAATTTCTTTACGCATGTCACA
    TTTGATATACCGTTATTTATATTAGAACCTGTTCATATGACAACAGCAGTATTCTTTGAT
    TTCGGTGTTTTATGTGCAGTAGTTGGTACTGTCATGACAATTATTATTTCGATTGGAGAG
    AACGAATAG
    60
    120
    180
    240
    300
    360
    420
    429

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0821 [new locus tag: NWMN_RS04640 ]
  • symbol: MnhB
  • description: putative monovalent cation/H+ antiporter subunit B
  • length: 142
  • theoretical pI: 4.88938
  • theoretical MW: 15681.7
  • GRAVY: 1.19155

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00943; HMM-score: 160.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined MnhB; Domain related to MnhB subunit of Na+/H+ antiporter (PF04039; HMM-score: 100.6)
    and 1 more
    NfeD-like (CL0252) NfeD; NfeD-like C-terminal, partner-binding (PF01957; HMM-score: 7.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004243
    • TAT(Tat/SPI): 0.000461
    • LIPO(Sec/SPII): 0.00489
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGLLFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFDIPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_0821&oldid=57388"