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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0819 [new locus tag: NWMN_RS04630 ]
  • pan locus tag?: SAUPAN003050000
  • symbol: mnhD
  • pan gene symbol?: mnhD1
  • synonym:
  • product: putative monovalent cation/H+ antiporter subunit D

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0819 [new locus tag: NWMN_RS04630 ]
  • symbol: mnhD
  • product: putative monovalent cation/H+ antiporter subunit D
  • replicon: chromosome
  • strand: -
  • coordinates: 906085..907581
  • length: 1497
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGATTGAAAGTAATATGCTTGTGTTAACGCTAGTTATTCCAGTAATTACAGCCATATTA
    CTTGTCTTTATTGGAAAGCGTCCTATTATAAAGCGTTATGTCGCACTAGGCGGTACATTA
    TTAACCTTGGTCGCGGCAATTATCAATTTAGCAAATGTTGTTAAACATGGACCAATACGC
    GTAGAGCTAGGTTCATGGAAAGCACCATACAGTATTGTCTTTGTATTAGACATATTCAGT
    GCCCTACTAATAATTACTAGTATTATTATTACAGCTATTGTGATTTTATATTCATACCAA
    ACAATTGGTATTGAACGTGAAAGATATTATTATTATTTTTCAGTACTATTTATGTTAATA
    GGTATCATTGGTGCATTCACAACTGGTGACATTTTCAACCTATTCGTATTTTTCGAAGTA
    TTTTTAATGTCATCTTATTTCTTATTAGTAATTGGTTCTACAAAAATTCAACTACAAGAA
    ACAATAAAATATGTTCTTGTTAACGTTGTATCGTCATCCTTCTTCGTAATGGGTGTAGCG
    ATTTTATATTCAGTAGTAGGAACATTGAATCTAGCAGATATAAGCAATAAATTAGCTAAC
    CTTTCAGCGCATGACAGTGGCTTAGTCAATATCGTTTTCATACTATTTATCTTTGTTTTT
    GCTACAAAAGCTGGCGTATTCCCAATGTTTGTTTGGTTACCTAGTGCATACTATGCACCA
    CCAATTCCAATCATTGCATTCTTTGGTGCTTTGTTAACAAAAGTTGGTGTTTATGCAATC
    GCTAGAACATTGAGCCTATTCTTTAGTGATAATGTAAGCTTTTCACATTATGTCATTTTA
    TTCTTAGCACTATTAACGATCATCTTTGGTTGTGTCGGTGCTGTTGCATATGCCAACATT
    AAAAAAATCATCCTATACAATGTCATGATTGCTGTAGGTGTGATCTTAGTCGGCGTTGCA
    ATGATGACAGAGTCAGGTATGATTGGGGCTATTTACTATACATTGCACGACATGTTAGTA
    AAACTTGCATTATTCTTGCTTATCGGAATTATGATTAAAATTACAGGCACTGCTGATTTA
    AGACAATTTGGTGGCTTAATTAAACGATATCCTGTACTTGGTTGGTCATTCTTTATCGCA
    GCATTAAGTTTAGCTGGCATTCCCCCACTCAGTGGCTTTTATGGAAAATTTTTTATTGTC
    CAATCAACATTTGAAAGAGGATTTTATTTAAGTGGTGTCATCGTCTTATTATCAAGTTTA
    GTCGTATTATATTCTGTCATTCGCATATTCCTACAAGGCTTTTTCGGTCAACCAAAAGGA
    TATGATCTTAATAACAAAGTCGACGTCAAATATTTAACTACGATTGCAATCGTTGCAGTT
    GTGATAACTGTTTTATATGGATTGTCAGCAGATTACTTATATCCAATGGTAAAAGCTGGT
    GCAGAAACATTCTACAATCCATCAACCTACGTTAAAGCTGTATTAGGAGGTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0819 [new locus tag: NWMN_RS04630 ]
  • symbol: MnhD
  • description: putative monovalent cation/H+ antiporter subunit D
  • length: 498
  • theoretical pI: 9.70179
  • theoretical MW: 54755.5
  • GRAVY: 1.12691

⊟Function[edit | edit source]

  • reaction:
    EC 1.6.5.3?  ExPASy
    NADH:ubiquinone reductase (H+-translocating) NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out)
  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent K+:H+antiporter (TIGR00944; HMM-score: 765.1)
    and 5 more
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 191.1)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 159.2)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 111.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 92.3)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 74.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 174.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010689
    • TAT(Tat/SPI): 0.000932
    • LIPO(Sec/SPII): 0.003493
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQETIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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