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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1012 [new locus tag: SACOL_RS05170 ]
  • pan locus tag?: SAUPAN003179000
  • symbol: SACOL1012
  • pan gene symbol?: —
  • synonym:
  • product: ribosomal large subunit pseudouridine synthase D

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1012 [new locus tag: SACOL_RS05170 ]
  • symbol: SACOL1012
  • product: ribosomal large subunit pseudouridine synthase D
  • replicon: chromosome
  • strand: +
  • coordinates: 1019249..1020103
  • length: 855
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAATTTAAGTATCATATATCACAACAAGAAACTGTTAAAACTTTTTTAGCACGACAT
    GATTTTTCTAAGAAGACAGTGAGCGCCATTAAAAATAATGGCGCTTTAATTGTTAATGAT
    GAACCAGTCACAGTGCGTAAGCAATTAATGACAAATGATATATTAGAAATTCATTTACCG
    CGAGAAATACCGAGTGTTAATTTAATACCTTATGCTCGTAAGCTAGAAGTATTGTATGAA
    GATGCTTTTATCATCATAGTTACTAAACCAAACAATCAAAATTGTACGCCTTCGAGAGAA
    CATCCTCATGAAAGTTTAATCGAACAAGTACTATATCATTGTCAGGAACATGGTGAAAAT
    ATTAACCCACATATTGTTACGCGTCTAGATCGTAATACAACTGGTATTGTGATATTCGCT
    AAATATGGACATATCCATCATTTATTTTCTAAAGTAAACTTGAAAAAAATATATACTTGC
    CTTGTATATGGTAAAACCCATACATCTGGTATTATTGAAGCTAATATTAGACGGTCAAAG
    GATAGGATTATAACTAGAGAAGTTGCCTCGGATGGTAAATACGCTAAAACATCTTATGAA
    GTAATAAATCAGAATGATAAATACAGTTTATGCAAAGTTCATTTGCATACGGGACGTACA
    CATCAAATTCGTGTACATTTTCAACATATTGGGCATCCAATTGTGGGAGATTCTTTGTAT
    GATGGTTTTCATGACAAAATTCATGGTCAAGTACTGCAATGTACGCAAATATATTTTGTT
    CATCCAATCAATAAGAACAATATTTATATTACAATTGATTATAAGCAATTACTTAAATTA
    TTCAATCAACTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    855

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1012 [new locus tag: SACOL_RS05170 ]
  • symbol: SACOL1012
  • description: ribosomal large subunit pseudouridine synthase D
  • length: 284
  • theoretical pI: 9.57231
  • theoretical MW: 33002.7
  • GRAVY: -0.396127

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase, RluA family (TIGR00005; EC 5.4.99.-; HMM-score: 182)
    and 2 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase Rlu family protein, TIGR01621 (TIGR01621; EC 5.4.99.-; HMM-score: 74.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase (TIGR00093; EC 5.4.99.-; HMM-score: 31.6)
  • TheSEED  :
    • Similar to ribosomal large subunit pseudouridine synthase D, Bacillus subtilis YjbO type
    Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial  Similar to ribosomal large subunit pseudouridine synthase D, Bacillus subtilis YjbO type
  • PFAM:
    PseudoU_synth (CL0649) PseudoU_synth_2; RNA pseudouridylate synthase (PF00849; HMM-score: 88.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003502
    • TAT(Tat/SPI): 0.000284
    • LIPO(Sec/SPII): 0.000619
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVTVRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHPHESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTCLVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTGRTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQLLKLFNQL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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