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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1086 [new locus tag: SACOL_RS05540 ]
  • pan locus tag?: SAUPAN003292000
  • symbol: SACOL1086
  • pan gene symbol?: thiV
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1086 [new locus tag: SACOL_RS05540 ]
  • symbol: SACOL1086
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1096635..1097210
  • length: 576
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGTCAAAAGGTTTAAAGCTATCTGAAATACTAGTAACCGTACTTATTTCAGTAGTATTC
    GCAGTTATTTACAATTTATGGTGGTTTGTTTACAATGGTGTGCAAGCTACAGGCCTTCAC
    CTCGAACAATTAACAAACGGGGTTTGGTTCATGGCAGCGATTGTCTGCTATCTAATTATT
    CCAAAACCAGGTATTGCTTTACTGGCTGAATTTGCTGCAGGTGCCGGCGAAACTATCATT
    ATGGGTCGTTTCGATATTCCGACAATCGTTTACGCTTTTATACAAGGTTTAGCGTGTGAA
    CTTGTCTTTGCAATTTTTAAATATCAATCACGTTCAGTTATGGTAGCTATGTTAGCAGGT
    TTTTGTACAGCAATCGCGGCTTTCCCGATAGATTATTTCTATGGTTATTTAAATGAAGTT
    GCAGGTTGGAACTTAACATTATTCATCGTATTTAGATTAATTAGTGGTGCAGTAGTCGCC
    GGCGTCCTATCTTACCTTTTAGTAAAAGCTTTAGATAAGACAGGCGTAACAAAATTATTT
    AGACCAGCTGCTAAAGAAGATTACGACAACTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    576

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1086 [new locus tag: SACOL_RS05540 ]
  • symbol: SACOL1086
  • description: hypothetical protein
  • length: 191
  • theoretical pI: 7.172
  • theoretical MW: 21001.9
  • GRAVY: 0.928796

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Thiamin-regulated hydroxymethylpyrimidine ECF transporter, substrate-specific component YkoE
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Substrate-specific component YkoE of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine
    and 1 more
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Substrate-specific component YkoE of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine
  • PFAM:
    no clan defined ABC_cobalt; ABC-type cobalt transport system, permease component (PF09819; HMM-score: 108.1)
    and 1 more
    DUF3082; Protein of unknown function (DUF3082) (PF11282; HMM-score: 9.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007353
    • TAT(Tat/SPI): 0.000184
    • LIPO(Sec/SPII): 0.005969
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQLTNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELVFAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVAGVLSYLLVKALDKTGVTKLFRPAAKEDYDNL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: THI-box (transcription termination) regulon
    THI-box(RNA)important in Thiamine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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