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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_0945 [new locus tag: NWMN_RS05290 ]
- pan locus tag?: SAUPAN003292000
- symbol: NWMN_0945
- pan gene symbol?: thiV
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_0945 [new locus tag: NWMN_RS05290 ]
- symbol: NWMN_0945
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1049808..1050383
- length: 576
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5330563 NCBI
- RefSeq: YP_001331979 NCBI
- BioCyc:
- MicrobesOnline: 3706496 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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541ATGTCAAAAGGTTTAAAGCTATCTGAAATACTAGTAACCGTACTTATTTCAGTAGTATTC
GCAGTTATTTACAATTTATGGTGGTTTGTTTACAATGGTGTGCAAGCTACAGGCCTTCAC
CTCGAACAATTAACAAACGGGGTTTGGTTCATGGCAGCGATTGTCTGCTATCTAATTATT
CCAAAACCAGGTATTGCTTTACTGGCTGAATTTGCTGCAGGTGCCGGCGAAACTATCATT
ATGGGTCGTTTCGATATTCCGACAATCGTTTACGCTTTTATACAAGGTTTAGCGTGTGAA
CTTGTCTTTGCAATTTTTAAATATCAATCACGTTCAGTTATGGTAGCTATGTTAGCAGGT
TTTTGTACAGCAATCGCGGCTTTCCCGATAGATTATTTCTATGGTTATTTAAATGAAGTT
GCAGGTTGGAACTTAACATTATTCATCGTATTTAGATTAATTAGTGGTGCAGTAGTCGCC
GGCGTCCTATCTTACCTTTTAGTAAAAGCTTTAGATAAGACAGGCGTAACAAAATTATTT
AGACCAGCTGCTAAAGAAGATTACGACAACTTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_0945 [new locus tag: NWMN_RS05290 ]
- symbol: NWMN_0945
- description: hypothetical protein
- length: 191
- theoretical pI: 7.172
- theoretical MW: 21001.9
- GRAVY: 0.928796
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: no clan defined ABC_cobalt; ABC-type cobalt transport system, permease component (PF09819; HMM-score: 108.1)and 1 moreDUF3082; Protein of unknown function (DUF3082) (PF11282; HMM-score: 9.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 4
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007353
- TAT(Tat/SPI): 0.000184
- LIPO(Sec/SPII): 0.005969
- predicted transmembrane helices (TMHMM): 5
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQLTNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELVFAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVAGVLSYLLVKALDKTGVTKLFRPAAKEDYDNL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: THI-box (transcription termination) regulon
THI-box (RNA) important in Thiamine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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