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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1128 [new locus tag: SACOL_RS05760 ]
  • pan locus tag?: SAUPAN003346000
  • symbol: SACOL1128
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1128 [new locus tag: SACOL_RS05760 ]
  • symbol: SACOL1128
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1139278..1140177
  • length: 900
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGAATAAATCAAATAACCATGCTGAAAATCCAAAGCCTAAAGAAGGTGTTGGTACATGG
    GTAGGTAAAGATATTAAAGTGCTTACTTCTAAATTTGGACAAGCAGATCGTGTCTACCCT
    TTTAGAGATGGTTACAAAAATTATGTGTTTAAAGACAAAAACAGTTATTACATTGTTTCA
    ACTAAACGTGAAGAAATCGTTTCAGTGTATGCTACAGGTGAGAAAGTCAATGTTAGTCCG
    TTAAAAATAGGACAACATTCTGCAGAAATTTTTAATCATACAAGTATTAATCCAGAACCG
    TCCTTTAAAGTTGATGGTAAAAAATATGAATTTGAACTTTCAGATGAAGATTTAAAAACA
    CAAACACTGATTAAATATGGCGACATATATGCACAAGTGTATTCTGATCAACAATCGAAA
    AAGGTGCTAAGTGTACGATTTTTAACAAAAGAAATGTTAGCAGATATTGAACCTTATCAA
    TTAAATTCTAATTCTACGTCAGAAGAGCATAATAAGCGTCCAGTTGAGCAAAATCCAAAT
    CAATTAATTTCTCTTTATGAAGTAACGAATGAAATGAGAAAATTAAAAGGATTAAAACCA
    TTGAAAATCAATAGCGATTTAGCACATATTGCATCTAATAACTTATATGAAGCGACCTCT
    AATGGTTCTGATAGTGTTGAATTTACAGAGGACGCATTAAGAGGGCAATTAGATAAAAAT
    CACGTTACTTATAAAACAACTGCTCAAAATGTTGGTTATGCGTTTAATGATGTACCAACA
    TTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGTCTATTAAATTCAAAATAC
    GATGAGATGGGTGGAGATGTAATGAGAGATTATTATTCACTAATTTTCTTAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1128 [new locus tag: SACOL_RS05760 ]
  • symbol: SACOL1128
  • description: hypothetical protein
  • length: 299
  • theoretical pI: 6.97825
  • theoretical MW: 34265.1
  • GRAVY: -0.759866

Function[edit | edit source]

  • TIGRFAM:
    uncharacterized protein, YkwD family (TIGR02909; HMM-score: 45.4)
  • TheSEED  :
    • Allergen V5/Tpx-1 related
  • PFAM:
    no clan defined CAP_assoc_N; CAP-associated N-terminal (PF14504; HMM-score: 140.5)
    and 2 more
    CAP (CL0659) CAP; Cysteine-rich secretory protein family (PF00188; HMM-score: 41.6)
    Peptidase_MA (CL0126) DUF45; Protein of unknown function DUF45 (PF01863; HMM-score: 12.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.039943
    • TAT(Tat/SPI): 0.001757
    • LIPO(Sec/SPII): 0.007666
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSYYIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELSDEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNKRPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTEDALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVMRDYYSLIFLEK

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]