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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1142 [new locus tag: SACOL_RS05830 ]
  • pan locus tag?: SAUPAN003362000
  • symbol: isdD
  • pan gene symbol?: isdD
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1142 [new locus tag: SACOL_RS05830 ]
  • symbol: isdD
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1149973..1151049
  • length: 1077
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGAAATGTTAAACAAATTGCTACAAAATCTATTATAGCTATTATTAGCTTAGGTATA
    CTTACATATACAACAATGATTGGTAGCGTGTTGGCTGATGAGATAAAATATCCATCAGCC
    AAATTTAATCAACCTGAAGCAAAAGATAAAACAGAATTAACTACATCAATTTTTGATGAA
    AAGATAAAAGAGAATAAAGCGTTAGAGTTACTAATTTTTAATCAAGAAAATAAAAATGTA
    ACTGAGGAACAACAACTAGTTGACGAAAAGGCGCAATTGATTTCAGATATGACTGGTAAA
    ATTTACTTGCAAGTAAAGCTAAAAGGTCAAATAGATAAAGAACAACTTGTTTTTCAAAAT
    GACAAAAATGAAGAATTTCCTTTTGTTATAAAAGATGAAAAGGATGACACAATAGTAAGA
    ATTTTAATTGAACAGCATATGGATAAAATCAATATGCATGTTAAAACGTTGGCTGAAAAG
    AAAAATCTAGATAACAAAGAAATGGTGTATTCTATTCATTTTAAAGAGAAAAAAGTACAA
    CATGATGATGCAAAAGAAGTGCCTTCAAAACATCAAAATCAAGAAAATAATCAAGATCAG
    CTTAAAAAAGATATTGATGACAAAAAAGATAGTCAAAAATCAGATACTAAGGAAAGACGT
    ACTAGCCTTTTTACTGAAAAAGGATTAAATGATATTCCTGTACAAAAAGATAAAGTGCAA
    CAAGACAGTAATAAAAAGATTGAAAATGAGCGACCTAAAGCATCAGGTACATTAAAAGTT
    GAAAATAGCCCTCCAACAATAAAAAAGGTTGAAAATAATCACAAAGAGCAACCGAAACAT
    AAAGATGAAAAATCAAAAAAGGAAAAGAAAAAAGTAGTTGAAAAAGAAAAAGCGTTACCA
    GCTTTTAATAGAGATGATGATAGCAAGAATAGTAGTCAATTATCTAGTGATATTAAAGAA
    CTTGATGAACCAAATCATAAAAAGCAATATATGTTATTTGCAGCTGGCATTGTGTTAGCA
    ACTATTTTACTTATTTCGGCACATTTATACAGCAGAAAGAGAGGTAACCAAGTTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1077

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1142 [new locus tag: SACOL_RS05830 ]
  • symbol: IsdD
  • description: hypothetical protein
  • length: 358
  • theoretical pI: 9.51057
  • theoretical MW: 41347.8
  • GRAVY: -0.992458

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED:  
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme transporter IsdDEF, membrane component IsdD
  • PFAM:

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • LocateP: C-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: SVLADEIK
  • SignalP: Signal peptide SP(Sec/SPI) length 32 aa
    • SP(Sec/SPI): 0.983168
    • TAT(Tat/SPI): 0.001823
    • LIPO(Sec/SPII): 0.001339
    • Cleavage Site: CS pos: 32-33. VLA-DE. Pr: 0.9680
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQPEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIYLQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEKKNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKERRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKEQPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFAAGIVLATILLISAHLYSRKRGNQV

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Michelle L Reniere, Eric P Skaar
    Staphylococcus aureus haem oxygenases are differentially regulated by iron and haem.
    Mol Microbiol: 2008, 69(5);1304-15
    [PubMed:18643935] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]