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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1142 [new locus tag: SACOL_RS05830 ]
- pan locus tag?: SAUPAN003362000
- symbol: isdD
- pan gene symbol?: isdD
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1142 [new locus tag: SACOL_RS05830 ]
- symbol: isdD
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 1149973..1151049
- length: 1077
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236098 NCBI
- RefSeq: YP_186005 NCBI
- BioCyc: see SACOL_RS05830
- MicrobesOnline: 912609 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGAGAAATGTTAAACAAATTGCTACAAAATCTATTATAGCTATTATTAGCTTAGGTATA
CTTACATATACAACAATGATTGGTAGCGTGTTGGCTGATGAGATAAAATATCCATCAGCC
AAATTTAATCAACCTGAAGCAAAAGATAAAACAGAATTAACTACATCAATTTTTGATGAA
AAGATAAAAGAGAATAAAGCGTTAGAGTTACTAATTTTTAATCAAGAAAATAAAAATGTA
ACTGAGGAACAACAACTAGTTGACGAAAAGGCGCAATTGATTTCAGATATGACTGGTAAA
ATTTACTTGCAAGTAAAGCTAAAAGGTCAAATAGATAAAGAACAACTTGTTTTTCAAAAT
GACAAAAATGAAGAATTTCCTTTTGTTATAAAAGATGAAAAGGATGACACAATAGTAAGA
ATTTTAATTGAACAGCATATGGATAAAATCAATATGCATGTTAAAACGTTGGCTGAAAAG
AAAAATCTAGATAACAAAGAAATGGTGTATTCTATTCATTTTAAAGAGAAAAAAGTACAA
CATGATGATGCAAAAGAAGTGCCTTCAAAACATCAAAATCAAGAAAATAATCAAGATCAG
CTTAAAAAAGATATTGATGACAAAAAAGATAGTCAAAAATCAGATACTAAGGAAAGACGT
ACTAGCCTTTTTACTGAAAAAGGATTAAATGATATTCCTGTACAAAAAGATAAAGTGCAA
CAAGACAGTAATAAAAAGATTGAAAATGAGCGACCTAAAGCATCAGGTACATTAAAAGTT
GAAAATAGCCCTCCAACAATAAAAAAGGTTGAAAATAATCACAAAGAGCAACCGAAACAT
AAAGATGAAAAATCAAAAAAGGAAAAGAAAAAAGTAGTTGAAAAAGAAAAAGCGTTACCA
GCTTTTAATAGAGATGATGATAGCAAGAATAGTAGTCAATTATCTAGTGATATTAAAGAA
CTTGATGAACCAAATCATAAAAAGCAATATATGTTATTTGCAGCTGGCATTGTGTTAGCA
ACTATTTTACTTATTTCGGCACATTTATACAGCAGAAAGAGAGGTAACCAAGTTTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1142 [new locus tag: SACOL_RS05830 ]
- symbol: IsdD
- description: hypothetical protein
- length: 358
- theoretical pI: 9.51057
- theoretical MW: 41347.8
- GRAVY: -0.992458
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 2
- LocateP: C-terminally anchored (with CS)
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: -2
- Predicted Cleavage Site: SVLADEIK
- SignalP: Signal peptide SP(Sec/SPI) length 32 aa
- SP(Sec/SPI): 0.983168
- TAT(Tat/SPI): 0.001823
- LIPO(Sec/SPII): 0.001339
- Cleavage Site: CS pos: 32-33. VLA-DE. Pr: 0.9680
- predicted transmembrane helices (TMHMM): 2
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQPEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIYLQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEKKNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKERRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKEQPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFAAGIVLATILLISAHLYSRKRGNQV
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Integral membrane [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: Fur* (repression) regulon
Fur* (TF) important in Iron homeostasis; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Michelle L Reniere, Eric P Skaar
Staphylococcus aureus haem oxygenases are differentially regulated by iron and haem.
Mol Microbiol: 2008, 69(5);1304-15
[PubMed:18643935] [WorldCat.org] [DOI] (I p)