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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1326 [new locus tag: SACOL_RS06755 ]
  • pan locus tag?: SAUPAN003623000
  • symbol: SACOL1326
  • pan gene symbol?: hflX
  • synonym:
  • product: GTP-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1326 [new locus tag: SACOL_RS06755 ]
  • symbol: SACOL1326
  • product: GTP-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1343457..1344695
  • length: 1239
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCTCAGCAACAAATTCATGATACTAAAAATAAACTAGAAAAAGCTGTCTTAGTCGGT
    GTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCT
    TTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTA
    GATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTC
    AAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTA
    AATGAAGCTTTAGGTGTAAAAATTATTGATAGAACTCAGTTGATTCTTGAAATATTTGCA
    TTAAGAGCAAGAAGTAAAGAAGGTAAATTGCAAGTAGAGCTAGCACAACTTGATTATTTA
    TTACCTAGATTGCAAGGCCATGGTAAAAGCCTTTCTCGTTTAGGTGGCGGTATTGGAACT
    AGAGGCCCTGGTGAAACGAAGTTAGAGATGGATCGCAGACATATTCGAACTCGTATGAAT
    GAAATTAAACATCAATTGCGGACGGTAGAAGAACATCGCGAAAGATATCGAAATAAAAGA
    AATCAAAATCAGGTGTTTCAAGTAGCTTTAGTTGGTTATACAAATGCTGGTAAATCATCA
    TGGTTTAATGTTTTAGCAAATGAAGAGACGTATGAAAAAGATCAATTATTTGCAACGTTA
    GATCCTAAAACACGACAAATTCAAATAAATGATGGATTTAATTTAATTATTTCAGATACT
    GTTGGTTTTATACAGAAACTACCTACGACGTTAATTGCAGCTTTTAAATCAACTTTAGAA
    GAGGCTAAAGGTGCAGATTTATTAGTACATGTCGTAGATAGTAGCCATCCTGAATACCGT
    ACGCAGTATGACACAGTTAATGATTTAATCAAACAATTAGATATGAGTCATATTTCTCAA
    ATAGTTATTTTTAATAAAAAGGACTTATGTGATCATGCATCAAATCGTCCAGCAAGTGAT
    TTGCCTAATGTTTTTGTTTCTTCTAAAAATGATGGTGATAAATTACTTGTTAAGACGTTA
    TTTATTGATGAAATCAAAAGGCAATTAACTTATTATGATGAGACAATTGCGACGAATAAT
    GCAGATCGATTATATTTTCTAAAACAACATACATTAGTGACTGAACTTAAATATGATGAA
    ATTGAAAATGTTTATCGTATAAAAGGATTTAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1239

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1326 [new locus tag: SACOL_RS06755 ]
  • symbol: SACOL1326
  • description: GTP-binding protein
  • length: 412
  • theoretical pI: 7.1298
  • theoretical MW: 47335.4
  • GRAVY: -0.551214

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 466.4)
    and 11 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 56.6)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 45.5)
    Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 42.6)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 40.4)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 35.7)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 32.1)
    Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 29.3)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 27.4)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 20.1)
    Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 15.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 10.8)
  • TheSEED  :
    • Ribosome LSU-associated GTP-binding protein HflX
    Protein Metabolism Protein biosynthesis Universal GTPases  GTP-binding protein HflX
    and 1 more
    Stress Response Stress Response - no subcategory Hfl operon  GTP-binding protein HflX
  • PFAM:
    no clan defined GTP-bdg_M; GTP-binding GTPase Middle Region (PF16360; HMM-score: 110.3)
    GTP-bdg_N; GTP-binding GTPase N-terminal (PF13167; HMM-score: 108.2)
    and 12 more
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 66.7)
    Arf; ADP-ribosylation factor family (PF00025; HMM-score: 23.4)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 22.2)
    Gtr1_RagA; Gtr1/RagA G protein conserved region (PF04670; HMM-score: 22.1)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 21.7)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 19.2)
    Thioredoxin (CL0172) DUF4174; Domain of unknown function (DUF4174) (PF13778; HMM-score: 16.2)
    P-loop_NTPase (CL0023) SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 15.7)
    ABC_tran; ABC transporter (PF00005; HMM-score: 14.7)
    Dynamin_N; Dynamin family (PF00350; HMM-score: 13.8)
    Chelatase (CL0043) ZnuA; Zinc-uptake complex component A periplasmic (PF01297; HMM-score: 13)
    no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006334
    • TAT(Tat/SPI): 0.00045
    • LIPO(Sec/SPII): 0.00074
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSETCQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNEALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGTRGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGKSSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFKSTLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHASNRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTLVTELKYDEIENVYRIKGFKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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