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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1524 [new locus tag: SACOL_RS07770 ]
  • pan locus tag?: SAUPAN003948000
  • symbol: SACOL1524
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1524 [new locus tag: SACOL_RS07770 ]
  • symbol: SACOL1524
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1563335..1564288
  • length: 954
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    TTGCAACACATTATAAAAACAGCATTACAACAAACATTTAACTATAAAACAAATAAAAGT
    ATTTACAATATCTTAGTTGGTAAGAAATCTCACCAAACCTTTTTTGACGCTTGTAGTCAA
    CAACAGTTGTCATTATATCACAGTTTACCACTATTAAAATATCCGTCTTTTGAGCTATTT
    TTAGAAAAAATCAATGAATTTAATGCTGAAATGGAAATCATGTTGCATCCTAGATATACA
    TTTGAAAGCATGGGGCAAACATTTCAAGCAATTCAACTATTAGTGCAAACCATGTCTAAT
    ACCAAACAACATGTTTTTCATTTTGTACCAATCTCTCAAAATAATAAGATACAAGAAACT
    GTAAAGATTGTCTATAACTACATTAAAGAAAACCAATTACAAATTGATTTTGAAAATGAA
    CTACATAACTTGTTTAAAGCAATAACTTTAAAAGGGCCATGTTACTTACATTATTATTTG
    CAAGGCTATGATGAACCAATGTATACGAGACAGCAAGTTAGTTTAATAGAAAAGCTATCT
    CAACAGCAATTGTTTGAATACGAAATGAATAATTTAGTGACAATGATGTTTGAATTAGAA
    AGTGGAGAATATACTATTTTATCAAAAATAATAATGAAACCTACATTATTAAATCAAACT
    TATATTACTTATACAAAATTGCTTGAACAATTCACGATGGAAGATATAGCGGCTCAACAA
    CAAGTTAAAATCAATACTATCGAAGATCATGTACTTGAAATCTTAATCAAAGGTTACATG
    TCTAATTACGATGATTATGTTGAACTAGAAGATCAACTCCAGTTTTTGAATTTTTATCAA
    CAGCATCGTGGCGAACGATTAAAATTTTACAAAGAACAATTTGACACGTTATCATATTTT
    CAATTAAAAGTATTAATCGTTGGATTTGAAAGAGGTGATCTGAATGTTGCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    954

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1524 [new locus tag: SACOL_RS07770 ]
  • symbol: SACOL1524
  • description: hypothetical protein
  • length: 317
  • theoretical pI: 5.79327
  • theoretical MW: 37849.2
  • GRAVY: -0.332808

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED:  
    hypothetical protein YpbB
  • PFAM:
    HTH (CL0123) HTH_40; Helix-turn-helix domain (PF14493; HMM-score: 52.1)
    and 1 more
    no clan defined PolC_DP2; DNA polymerase II large subunit DP2 (PF03833; HMM-score: 9.1)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.023762
    • TAT(Tat/SPI): 0.000512
    • LIPO(Sec/SPII): 0.005869
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLSLYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTKQHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYLQGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLNQTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFLNFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]