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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1582 [new locus tag: SACOL_RS08060 ]
  • pan locus tag?: SAUPAN004100000
  • symbol: SACOL1582
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1582 [new locus tag: SACOL_RS08060 ]
  • symbol: SACOL1582
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1617734..1618789
  • length: 1056
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAATAATAAGCAAGTACATTTTAACACGATTTTCTAAAAACGGAAAGCATTTTGAT
    ATTCCTAAAGAACAACGCCGAGTAATTCCAAAATTTAAAAATCGTCGTAAGATGATTGTA
    ATTTGTTTTTACTCATTATTAGCTATTTTGTTAATTCTTTTATTAGCTTCTTTTATTAAA
    GCTACAAGAGCAAATAATGACAGTAAAGAAGCAGTTAATAAAACGAATATGATTCAAAAA
    AAGTATGAAGATAATGCGAATACAGTTCAGTATAGTCCTAAATTAAAGTTATATGCTGAT
    AAGTTTATTGATACGTATATGAATATCTCGAAAGATTCAAAAGAATTGGAATCTAGAGAA
    AAAGAATTGTTGAAATATTTTCCATCAGATTATAAAAAACCTGATGAAAAAATTTCAGAT
    ACAGAACGAAAATTGAATAGTAAAGAATTTTATAATATTAAACGTAAAGATAAACAAACA
    ATTATACAGTATATTGTTAATTATGATGTGAATATTACTGAAAAGAAAGAAGTTAAAGTT
    AAGAAGAAAAAGAAAAGTGAAAAAGATAAAGATGAGTATGAAACAAAAACAGAAGAAAAG
    CAACGTAAAGTAAATCAAAACATTTTAATAAATATACCTATTAAGAGTGAAAATAATAAG
    TATGTTGTTGTTGAATATCCTTATTTTACGCCAATCCCTGATAGTCAATTGAATAAAGCT
    AAAATGGTTGAGGATAATTTGAAAGACAATAAACGGGAAGACAATCCGAAAGCAAAAGCT
    TTTATTGAAGATTTCTTTAATAAATATGCTTCTAGTAAGTCTGATGATATGGCCTATTTA
    ATGGATAACCCTGAGGGCTTAGAAGGAACTAGAGAAGTTTCTCAGATAAGAGAAATTAGG
    TTATATCCAAAAGGCGATGATTATGTTGCTAAAGTTGAAATTTTGATGAAAGATAAAGAT
    TCTCCTTTGGAGAATTTGGAGCATTACACATTAGATATAACAAAAAAAGATGGTAAATAT
    TACGTTAAAAATATGACTAATTCTATTGGAGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1056

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1582 [new locus tag: SACOL_RS08060 ]
  • symbol: SACOL1582
  • description: hypothetical protein
  • length: 351
  • theoretical pI: 9.89778
  • theoretical MW: 41493.5
  • GRAVY: -0.931054

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • hypothetical protein transposon-related
  • PFAM:
    NTF2 (CL0051) TpcC; Conjugative transposon protein TcpC (PF12642; HMM-score: 113.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014885
    • TAT(Tat/SPI): 0.000655
    • LIPO(Sec/SPII): 0.005425
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFYSLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKFIDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQTIIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSENNKYVVVEYPYFTPIPDSQLNKAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDDMAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDITKKDGKYYVKNMTNSIGG

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]