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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1691 [new locus tag: SACOL_RS08625 ]
  • pan locus tag?: SAUPAN004241000
  • symbol: recJ
  • pan gene symbol?: recJ
  • synonym:
  • product: single-stranded-DNA-specific exonuclease RecJ

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1691 [new locus tag: SACOL_RS08625 ]
  • symbol: recJ
  • product: single-stranded-DNA-specific exonuclease RecJ
  • replicon: chromosome
  • strand: -
  • coordinates: 1721266..1723539
  • length: 2274
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAA
    TTAACATCAAAATTAAAGTTAACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATT
    ATTGATGAACAAGCAATAGAATCCATCATTTCAGATACAGATATCAATCATGATGCACTG
    CAATTGAGCGATATGACTAAGACTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
    AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCGCAACTACAATTTTAGTG
    ATAACATTACAACTTTTAGGTGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAA
    GGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATGATGAAGGCATAACATTAATC
    ATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
    GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCA
    ATCGTACATCCAATGCATCCATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGT
    GTTGCATACAAATTAGCACAAGCATTGATTGAAAATGTACCTGATTATTTTAAAGCATTA
    GTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
    AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAA
    GAAGCGGGATACAATGACAATATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGA
    CTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTTGCGAATTGTTGATGACTGAC
    GTCGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
    GATATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAAG
    GGAGATTTATTTTTACTTTTAGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTT
    GCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAATTTTAAATATTGATAGGGAG
    CAAAATCATGCAAAAGGCTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
    TCAGCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACG
    ATGGATATTGAAAATATAGAATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTA
    TCTAAAACAACTTCGTTAGATCCCGTAAAGCAAGTAGATGTATTATTAACAGAAAATGAT
    ATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
    AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATAGGTCAACAA
    AAAAATCATCTAAAATTGACACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAAT
    GGACACTTAGAACCTGAGTTACAAGATGAACAGCCAATTAATATATTAGGATCTGTTCAA
    ATCAACGAATGGAATGGTAATCAATCACCGCAAATAATTATTCAAGATATTGCGATGAAT
    GAACAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGAT
    GAAAATATTGTCGTGCTTATTCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTAT
    TATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTATTAAGAGATTTACCAACGTCA
    ATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
    CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGT
    TATAAAGCATTAATAACTAAACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGT
    CAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGTTGAAAGTTTTCTTAGACTTA
    AAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
    ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTA
    TTATATCAAGATTTTTCAGAAATAAAAAATTGGATAAAGTCACAATTGTCGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2274

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1691 [new locus tag: SACOL_RS08625 ]
  • symbol: RecJ
  • description: single-stranded-DNA-specific exonuclease RecJ
  • length: 757
  • theoretical pI: 4.90644
  • theoretical MW: 85610.4
  • GRAVY: -0.269485

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair single-stranded-DNA-specific exonuclease RecJ (TIGR00644; EC 3.1.-.-; HMM-score: 635.6)
  • TheSEED  :
    • Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)
    DNA Metabolism DNA repair DNA Repair Base Excision  Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)
    and 2 more
    DNA Metabolism DNA repair DNA repair, bacterial RecFOR pathway  Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)
    DNA Metabolism DNA replication DNA-replication  Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)
  • PFAM:
    no clan defined ssDNA-exonuc_C; Single-strand DNA-specific exonuclease, C terminal domain (PF10141; HMM-score: 195.9)
    and 3 more
    DHHA1; DHHA1 domain (PF02272; HMM-score: 76.8)
    HAD (CL0137) DHH; DHH family (PF01368; HMM-score: 46.4)
    Rep (CL0169) DUF1424; Putative rep protein (DUF1424) (PF07232; HMM-score: 10.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001917
    • TAT(Tat/SPI): 0.000126
    • LIPO(Sec/SPII): 0.000256
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVITLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLGVDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMAETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKQVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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