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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1732 [new locus tag: SACOL_RS08855 ]
  • pan locus tag?: SAUPAN004307000
  • symbol: SACOL1732
  • pan gene symbol?: dnaB
  • synonym:
  • product: replication initiation and membrane attachment protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1732 [new locus tag: SACOL_RS08855 ]
  • symbol: SACOL1732
  • product: replication initiation and membrane attachment protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1762986..1764386
  • length: 1401
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGACGACAAGCCTTCGAATTCGGCTTAAGACCAAAGGATCAATTCAAAGTTATGCAA
    CACTTTGATTTAAATACGAATCATTTAGAGGTATTGAATCGACTGTATACACCGCTAATT
    GGAACGCAAGCAGTGGGTTTATATCATTTTATGACTCAATTCGTTAAAGAAAGTCATAAT
    GAAACATTGATTCTTTCTCATTATATTTTTATGAATGAACTTAAAATTAATTTACTAGAG
    TTTAGACAACAAATGGACTTACTTGAAGCGATTGGATTGTTAAAGGCGTTTGTCAAACAT
    GATGAACAAGAAACACAATTTGTTTACCAATTAATTCAACCGCCTTCAGCACATTTATTT
    TTTAATGATCCAATGTTGTCAATCTTTTTATATAGTGAAGTCGAGCATCGTCGATTTCAT
    GAGTTGAAGAAATACTTTGAGTATCAACAAATTGATTTGAGTGAATTTAAACAGGTAACA
    AGACAATTTACTGATGTTTTTAAAGTGCCGTCTACAAAAATAGATATCGATACAAGTGAT
    ATTCCGATTAATGAACCGTACCAAGGTATCGATTTATCAAATGAATCTTTTGACTTTGAA
    ATGTTGAGACAAATGTTAGGTAAACATTTTATTAGTCAAGACATTGTTACCAAAGATGCA
    AAGCGTTTAATTACACAACTCGCGACTTTATATGGTTTAACAGCAGATGGCATGAAGCAT
    GTTATTTTAAATTCAATTACAAGTGGACAACAGTTATCCTTTGAAGAAATGCGTAAACAA
    GCACGCTCGTATTACTTAATGGAACACGAAAATCAAATGCCTAAATTACAGGTGAAATCT
    CCTGCTACATCATCTTCGGCGGGCAAATCTTCAGAAGTGAATCCAAAGCCACAAAGTGAT
    GAATGGTTTGAGTTACTTGAACAAACGAGTCCAATTGATATGTTAGCTTCTTGGTCTGAA
    TCAGAACCAACTATATCGCAAAAGACAATGGTTGAAGAATTAATTGAACGTGAAAAAATG
    TCTTTTGGCGTCATTAATATTTTGTTGCAATTTGTCATGTTAAAAGAAGATATGAAACTT
    CCTAAAGCGTATATTTTGGAAATAGCATCAAATTGGAAGAAAAAAGGTATTAAGACAGCT
    AAAGAAGCATATAATTACGCTAAAAAAGTTAATCAACCTAAAAATGAGGGTTCTAGCGGA
    AATTACCAAAAGCGTGGTTCATACTATGGGCAGCGTAATCGTATTTCAAAAGAAAAAACA
    CCTAAATGGTTAGAAAATAGAGATAAACCTAGTGAAGAAGATTCGGCTAAAGATAATAGC
    GTAGATGATCAACAATTAGAGCAAGATCGACAAGCATTTCTAGATAAATTATCTAAAAAA
    TGGGAGGAGGACAGTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1401

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1732 [new locus tag: SACOL_RS08855 ]
  • symbol: SACOL1732
  • description: replication initiation and membrane attachment protein
  • length: 466
  • theoretical pI: 6.12276
  • theoretical MW: 54532.6
  • GRAVY: -0.661159

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 20.3)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 20.3)
  • TheSEED:  
    Helicase loader DnaB
  • PFAM:
    no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 39.2)
    and 2 more
    DUF4144; protein structure with unknown function (PF13642; HMM-score: 14.3)
    SUIM_assoc; Unstructured region C-term to UIM in Ataxin3 (PF16619; HMM-score: 14.1)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002061
    • TAT(Tat/SPI): 0.00024
    • LIPO(Sec/SPII): 0.000242
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVTRQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]