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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1773 [new locus tag: SACOL_RS09070 ]
  • pan locus tag?: SAUPAN004379000
  • symbol: serA
  • pan gene symbol?: serA
  • synonym:
  • product: D-3-phosphoglycerate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1773 [new locus tag: SACOL_RS09070 ]
  • symbol: serA
  • product: D-3-phosphoglycerate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1811884..1813488
  • length: 1605
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAAGCAATTTAATGTACTCGTTGCAGATCCCATATCAAAAGATGGTATCAAAGCATTA
    TTAGATCACGAACAATTCAATGTAGATATTCAAACTGGCTTGTCCGAAGAAGCATTAATC
    AAAATTATACCTTCATACCATGCTTTAATCGTTCGTAGTCAAACTACGGTTACTGAAAAT
    ATCATAAATGCTGCTGATTCTTTAAAAGTAATCGCACGCGCCGGTGTTGGTGTAGATAAT
    ATTAATATTGATGCTGCAACATTAAAAGGTATTTTAGTTATTAATGCCCCAGATGGTAAT
    ACGATTTCAGCTACTGAACATACACTGGCAATGTTATTATCAATGGCACGAAATATTCCG
    CAAGCACACCAATCACTTACAAATAAAGAATGGAATCGAAATGCATTTAAAGGTACTGAG
    CTTTATCATAAAACATTAGGTGTCATTGGTGCTGGTAGAATTGGTTTAGGTGTTGCTAAA
    CGTGCGCAAAGTTTCGGAATGAAAATACTAGCTTTTGACCCTTACTTAACGGATGAAAAA
    GCAAAATCTTTAAGCATTACGAAGGCAACAGTTGATGAGATTGCCCAACATTCTGATTTC
    GTTACATTACATACACCACTAACACCTAAAACAAAAGGCTTAATTAATGCTGTCTTTTTT
    GCCAAAGCAAAACCTAGTTTGCAAATAATCAATGTGGCACGTGGTGGTATTATTGATGAA
    AAGGCGCTAATAAAAGCATTAGACGAAGGACAAATTAGTCGGGCAGCTATCGATGTGTTT
    GAACATGAACCTGCAACTGACTCGCCTCTTGTTGCACATGATAAAATTATTGTTACACCT
    CATTTGGGTGCTTCAACAGTCGAAGCTCAAGAAAAAGTGGCAATTTCTGTTTCAAATGAA
    ATCATCGAAATTTTAATTGATGGTACTGTAACGCATGCAGTGAATGCACCTAAAATGGAC
    TTAAGCAATATAGATGATACTGTAAAATCATTCATCAATTTAAGCCAAACAGTTGGTGAA
    TTAGCTATTCAATTAATGTACAATGCACCAAGCTCTATTAAAATTACGTACGGTGGCGAC
    TTAGCCTCTATTGATAGTAGTTTATTAACACGTACAATTATTACTCATATTTTAAAAGAT
    GATCTTGGTCCTGAAGTCAATATTATCAATGCTCTAATGTTGTTAAATCAACAACAAGTG
    ACATTAAATATTGAAAATAATAAAGCAGAGACAGGTTTTAGTAACTACTTAGAGGTAGAA
    CTATCAAACGATAGCGATTCCGTTAAAGTTGGCGCTTCTGTCTTTACAGGTTTCGGTCCA
    AGAATTGTTAGAATTAATAATTTTTCTGTAGACTTAAAGCCAAATCAATATCAAATTGTG
    TCATATCATAATGATACTCCAGGTATGGTAGGAAAAACTGGCGCATTGTTAGGTAAATAC
    AATATCAACATTGCATCTATGACTTTAGGTAGAACTGAAGCGGGCGGAGATGCGCTAATG
    ATTTTATCCGTTGATCAACCTGTTTCAAACAATATAATTGATGAACTTAAACAAGTTGGT
    GAATACAATCAAATTTTCACAACTGAATTGACGGTACAGTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1605

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1773 [new locus tag: SACOL_RS09070 ]
  • symbol: SerA
  • description: D-3-phosphoglycerate dehydrogenase
  • length: 534
  • theoretical pI: 5.30951
  • theoretical MW: 57618.4
  • GRAVY: 0.0337079

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.95?  ExPASy
    Phosphoglycerate dehydrogenase 3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 745.7)
    and 2 more
    Metabolism Energy metabolism Amino acids and amines L-serine dehydratase, iron-sulfur-dependent, beta subunit (TIGR00719; EC 4.3.1.17; HMM-score: 38.8)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis L-serine dehydratase, iron-sulfur-dependent, beta subunit (TIGR00719; EC 4.3.1.17; HMM-score: 38.8)
  • TheSEED  :
    • D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)
    and 2 more
    Amino Acids and Derivatives Alanine, serine, and glycine Serine Biosynthesis  D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Pyridoxine Pyridoxin (Vitamin B6) Biosynthesis  D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)
  • PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 209.9)
    and 5 more
    Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 119.2)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 38.6)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 18.7)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 16.9)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 14.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002437
    • TAT(Tat/SPI): 0.000141
    • LIPO(Sec/SPII): 0.000241
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIPSYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTISATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAKRAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAVFFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKIIVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINLSQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINALMLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSVDLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPVSNNIIDELKQVGEYNQIFTTELTVQS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 1861 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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