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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1797 [new locus tag: SACOL_RS09210 ]
  • pan locus tag?: SAUPAN004414000
  • symbol: SACOL1797
  • pan gene symbol?: —
  • synonym:
  • product: metallo-beta-lactamase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1797 [new locus tag: SACOL_RS09210 ]
  • symbol: SACOL1797
  • product: metallo-beta-lactamase
  • replicon: chromosome
  • strand: -
  • coordinates: 1844520..1845296
  • length: 777
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTTTGGTGTTGTTCCGAAGCCGTTGTGGTCAAAGCAATACAATGCAAATGAACGAAAT
    CAAATCAATTTACCGACACATCCAATTTTGATTCAAACGGCTCAATATAATTTGATTATA
    GATGCAGGTATTGGTAATGGTAAATTATCTGAAAAGCAATTACGTAATTTTGGAGTAGAT
    GAAGAAAGTCATATAATTGCTGATTTGGCAAATTATAATTTAACGCCAAAGGATATTGAT
    TATGTGCTAATGACACATATGCATTTTGATCATGCTGCCGGTTTGACTGATCAAGCAGGA
    CATGCAATTTTTGAAAATGCGATTCATGTTGTGCAACAAGATGAGTGGCATGAGTTTATT
    GCACCTAATATAAGAAGTAAATCAACTTACTGGGATAAGAATAAAGGCGATTATAGTAAC
    AAGTTGATTTTATTCGAAAAACATTTTGAACCGGTTCTGGGTATCAAGATGCAACATAGT
    GGAGGTCATAGCTTTGGCCACACGATTATTACGATTGAAAGTCAAGGAGATAAAGCAGTT
    CATATGGGTGATATATTCCCAACTACTGCACATAAAAATCCTCTATGGGTAACGGCATAT
    GATGATTATCCTATGCAATCGATTCGTGAAAAAGAACGCATGATACCATATTTTATTCAG
    CAACAATATTGGTTCTTGTTTTATCATGATGAAAACTACTTTGCTGTAAAATACAGCGAT
    AATGGTGAAAACATAGATGCATATATTTTACGTGAAACATTAGTTGATAATAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    777

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1797 [new locus tag: SACOL_RS09210 ]
  • symbol: SACOL1797
  • description: metallo-beta-lactamase
  • length: 258
  • theoretical pI: 5.9604
  • theoretical MW: 29914.4
  • GRAVY: -0.506589

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Transcription RNA processing ribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 19.9)
    Unknown function Enzymes of unknown specificity quinoprotein relay system zinc metallohydrolase 2 (TIGR04559; EC 3.-.-.-; HMM-score: 17.2)
    and 2 more
    phosphonate metabolism protein PhnP (TIGR03307; HMM-score: 13)
    arCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 13)
  • TheSEED  :
    • Metallo-beta-lactamase family protein
  • PFAM:
    Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 55.5)
    and 2 more
    Lactamase_B_3; Beta-lactamase superfamily domain (PF13483; HMM-score: 22.4)
    Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 22.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003859
    • TAT(Tat/SPI): 0.000171
    • LIPO(Sec/SPII): 0.000437
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFGVVPKPLWSKQYNANERNQINLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDIDYVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDYSNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLWVTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETLVDNN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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