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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1814 [new locus tag: SACOL_RS09300 ]
  • pan locus tag?: SAUPAN004452000
  • symbol: SACOL1814
  • pan gene symbol?: —
  • synonym:
  • product: lysophospholipase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1814 [new locus tag: SACOL_RS09300 ]
  • symbol: SACOL1814
  • product: lysophospholipase
  • replicon: chromosome
  • strand: -
  • coordinates: 1870425..1871252
  • length: 828
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTGGAAGTGGGAAGCTGAAAATGATGCGAAAGGCGTCGTTGTTATTGCTCATAATATA
    TTAGAACATACGGGAAGATATGCATATGTTATTACGATGTTAAGAAGAAATGGCTATCAT
    GTGATTATGGGAGATTTGCCTGGTCAAGGTCAAACTTCAAGAGCTAATAAAGGACAAATT
    GAAAACTTTCAAACGTATCACGAAAGTTTGCTAGACTGGTTAAAGATAGCTAATGAATAT
    AAAATTCCTACTTATGTATTAGGGGTAGGACTTGGCGGTTTGATTTTATTAAATTTACTT
    GAAAAAGTAGAATTGCCAATCGAGGGGATGATGTTAATTTCTCCTATGCTAGAACTTCAA
    AAGAATGGAAAAAATCGTAAAGATAAACTTGTTTCAAATATAGGTAAAATTTCAAAAGAC
    ACACGTTTTAATGTTGGTGTAGAACCAAAAGATTTAACACGTAATTTAGAAATTGTTGAA
    GAAACAGTTAACGATGGTTTAATGTTAAAAAAAGCAACGTATCATTGGTATAATACAATT
    AATGAGACGATGAAAGATACGATGGCTCATATTCATGACATTCAACCAATGCCAACGCTA
    TTAATGTATGGAACGAAGGATTTAATTGTTGATACTAGGGCAATTGATGAGTTTAAAGAA
    AAATATCAAACACCTGAATTATATTTTAAAGCTTGGCAAGGTTTTTACCACGAGGTTCAC
    AATGAGCCAGAACGTGATGAAGTAATGCGTTATATTCTGACTTTCTTAAATAACAGCGTC
    AATACAATGGGCTTTATTGTTGAAGATGACGAAATTGTAGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    828

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1814 [new locus tag: SACOL_RS09300 ]
  • symbol: SACOL1814
  • description: lysophospholipase
  • length: 275
  • theoretical pI: 5.40101
  • theoretical MW: 31865.4
  • GRAVY: -0.362182

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 32.7)
    and 2 more
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 20.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 20)
  • TheSEED  :
    • Lysophospholipase (EC 3.1.1.5)
    • Monoglyceride lipase (EC 3.1.1.23)
    Fatty Acids, Lipids, and Isoprenoids Triacylglycerols Triacylglycerol metabolism  Lysophospholipase (EC 3.1.1.5)
    and 1 more
    Fatty Acids, Lipids, and Isoprenoids Triacylglycerols Triacylglycerol metabolism  Monoglyceride lipase (EC 3.1.1.23)
  • PFAM:
    AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 144.7)
    and 5 more
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 48)
    DUF1057; Alpha/beta hydrolase of unknown function (DUF1057) (PF06342; HMM-score: 21.3)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 20.1)
    DLH; Dienelactone hydrolase family (PF01738; HMM-score: 17.8)
    LIDHydrolase; Lipid-droplet associated hydrolase (PF10230; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004227
    • TAT(Tat/SPI): 0.000733
    • LIPO(Sec/SPII): 0.000546
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMGDLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEKVELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVEETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEFKEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 59 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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