PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1710 [new locus tag: SAUSA300_RS09340 ]
• pan locus tag^?: SAUPAN004452000
• symbol: SAUSA300_1710
• pan gene symbol^?: —
• synonym:
• product: putative lysophospholipase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1710 [new locus tag: SAUSA300_RS09340 ]
• symbol: SAUSA300_1710
• product: putative lysophospholipase
• replicon: chromosome
• strand: -
• coordinates: 1892736..1893563
• length: 828
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914484 NCBI
• RefSeq: YP_494403 NCBI
• BioCyc: see SAUSA300_RS09340
• MicrobesOnline: 1293225 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTGGAAGTGGGAAGCTGAAAATGATGCGAAAGGCGTCGTTGTTATTGCTCATAATATA
  TTAGAACATACGGGAAGATATGCATATGTTATTACGATGTTAAGAAGAAATGGCTATCAT
  GTGATTATGGGAGATTTGCCTGGTCAAGGTCAAACTTCAAGAGCTAATAAAGGACAAATT
  GAAAACTTTCAAACGTATCACGAAAGTTTGCTAGACTGGTTAAAGATAGCTAATGAATAT
  AAAATTCCTACTTATGTATTAGGGGTAGGACTTGGCGGTTTGATTTTATTAAATTTACTT
  GAAAAAGTAGAATTGCCAATCGAGGGGATGATGTTAATTTCTCCTATGCTAGAACTTCAA
  AAGAATGGAAAAAATCGTAAAGATAAACTTGTTTCAAATATAGGTAAAATTTCAAAAGAC
  ACACGTTTTAATGTTGGTGTAGAACCAAAAGATTTAACACGTAATTTAGAAATTGTTGAA
  GAAACAGTTAACGATGGTTTAATGTTAAAAAAAGCAACGTATCATTGGTATAATACAATT
  AATGAGACGATGAAAGATACGATGGCTCATATTCATGACATTCAACCAATGCCAACGCTA
  TTAATGTATGGAACGAAGGATTTAATTGTTGATACTAGGGCAATTGATGAGTTTAAAGAA
  AAATATCAAACACCTGAATTATATTTTAAAGCTTGGCAAGGTTTTTACCACGAGGTTCAC
  AATGAGCCAGAACGTGATGAAGTAATGCGTTATATTCTGACTTTCTTAAATAACAGCGTC
  AATACAATGGGCTTTATTGTTGAAGATGACGAAATTGTAGAAATTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1710 [new locus tag: SAUSA300_RS09340 ]
• symbol: SAUSA300_1710
• description: putative lysophospholipase
• length: 275
• theoretical pI: 5.40101
• theoretical MW: 31865.4
• GRAVY: -0.362182

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 32.7)
  and 2 more
  Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 20.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 20)
• TheSEED  :
  • Lysophospholipase (EC 3.1.1.5)
  • Monoglyceride lipase (EC 3.1.1.23)
  Fatty Acids, Lipids, and Isoprenoids Triacylglycerols Triacylglycerol metabolism  Lysophospholipase (EC 3.1.1.5)
  and 1 more
  Fatty Acids, Lipids, and Isoprenoids Triacylglycerols Triacylglycerol metabolism  Monoglyceride lipase (EC 3.1.1.23)
• PFAM:
  AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 144.7)
  and 5 more
  Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 48)
  DUF1057; Alpha/beta hydrolase of unknown function (DUF1057) (PF06342; HMM-score: 21.3)
  Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 20.1)
  DLH; Dienelactone hydrolase family (PF01738; HMM-score: 17.8)
  LIDHydrolase; Lipid-droplet associated hydrolase (PF10230; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004227
  • TAT(Tat/SPI): 0.000733
  • LIPO(Sec/SPII): 0.000546
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161972 NCBI
• RefSeq: YP_494403 NCBI
• UniProt: A0A0H2XJR5 UniProt

⊟Protein sequence

• MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMGDLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEKVELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVEETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEFKEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications