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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1843 [new locus tag: SACOL_RS09455 ]
  • pan locus tag?: SAUPAN004510000
  • symbol: SACOL1843
  • pan gene symbol?: menC
  • synonym:
  • product: o-succinylbenzoic acid (OSB) synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1843 [new locus tag: SACOL_RS09455 ]
  • symbol: SACOL1843
  • product: o-succinylbenzoic acid (OSB) synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1897263..1898264
  • length: 1002
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGTTGACAGCTTTGCATTTTTATAAATATAGTGAGCCATTTAAGTCACAAATTGTA
    ACACCGAAAGTCACTTTAACGCATCGTGATTGTTTGTTTATCGAATTGATTGATGACAAA
    GGAAATGCATATTTCGGGGAATGTAACGCTTTTCAAACAGATTGGTATGATCATGAAACA
    ATTGCCTCAGTGAAACATGTAATTGAGCAATGGTTCGAAGATAATAGAAATAAATCATTT
    GAAACGTATGAAGCAGCACTAAAATTAGTAGATTCATTGGAAAATACGCCTGCTGCAAGG
    GCAACTATTGTCATGGCATTGTATCAAATGTTTCATGTACTGCCTTCATTTTCAGTAGCA
    TATGGAGCGACAGCGAGCGGCTTATCAAATAAACAACTAGAGTCATTAAAAGCAACAAAG
    CCGACCAGAATTAAATTAAAATGGACGCCTCAAATCATGCATCAAATTAGAGTGTTACGG
    GAATTAGACTTTCACTTTCAATTGGTTATAGATGCAAATGAGTCCTTGGATCGTCAAGAT
    TTCACACAGTTACAGTTATTAGCGCGTGAACAGGTTCTTTATATCGAAGAGCCATTTAAA
    GATATCTCCATGCTTGATGAAGTAGCGGATGGAACAATACCTCCAATAGCCCTTGATGAA
    AAGGCAACATCGTTGTTGGACATAATTAATTTGATAGAATTATACAATGTGAAAGTTGTT
    GTATTAAAACCATTTCGTCTAGGTGGCATTGATAAAGTGCAAACGGCAATTGATACTTTG
    AAAAGTCATGGTGCAAAAGTGGTTATTGGCGGCATGTACGAATATGGTTTAAGCCGCTAT
    TTTACAGCGATGCTTGCTCGTAAAGGTGATTATCCAGGAGATGTTACACCAGCAGGTTAT
    TATTTTGAACAAGATGTGGTGGCGCATTCAGGCATATTAAAAGAGGGGCGACTCGAATTT
    CGCCCCCCTTTAGTGGATATAACTCAATTACAACCATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

⊟Protein[edit | edit source]

Protein Data Bank: 2OKT
Protein Data Bank: 2OLA

⊟General[edit | edit source]

  • locus tag: SACOL1843 [new locus tag: SACOL_RS09455 ]
  • symbol: SACOL1843
  • description: o-succinylbenzoic acid (OSB) synthetase
  • length: 333
  • theoretical pI: 5.62288
  • theoretical MW: 37967.3
  • GRAVY: -0.146847

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.113?  ExPASy
    o-succinylbenzoate synthase (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone o-succinylbenzoate synthase (TIGR01928; EC 4.2.1.113; HMM-score: 443.2)
    and 3 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone o-succinylbenzoate synthase (TIGR01927; EC 4.2.1.113; HMM-score: 82.3)
    muconate and chloromuconate cycloisomerases (TIGR02534; EC 5.5.1.-; HMM-score: 28.5)
    Metabolism Amino acid biosynthesis Aspartate family S-methyl-5-thioribose-1-phosphate isomerase (TIGR00512; EC 5.3.1.23; HMM-score: 12.1)
  • TheSEED  :
    • O-succinylbenzoate synthase (EC 4.2.1.113)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Quinone cofactors Menaquinone and Phylloquinone Biosynthesis  O-succinylbenzoate synthase (EC 4.2.1.113)
    and 1 more
    Miscellaneous Miscellaneous - no subcategory Muconate lactonizing enzyme family  O-succinylbenzoate synthase (EC 4.2.1.113)
  • PFAM:
    Enolase_TIM (CL0256) MR_MLE_C; Enolase C-terminal domain-like (PF13378; HMM-score: 55.7)
    and 1 more
    MAAL_C; Methylaspartate ammonia-lyase C-terminus (PF07476; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: a divalent metal cation
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004548
    • TAT(Tat/SPI): 0.000118
    • LIPO(Sec/SPII): 0.000575
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAYFGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARATIVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLRELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIALDEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYGLSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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