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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1848 [new locus tag: SACOL_RS09480 ]
  • pan locus tag?: SAUPAN004518000
  • symbol: SACOL1848
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1848 [new locus tag: SACOL_RS09480 ]
  • symbol: SACOL1848
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1902466..1903092
  • length: 627
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAATTCAAAGCTATCGTTGCAATCACATTATCATTGTCACTATTAACTGCCTGTGGT
    GCTAATCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAA
    ACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGAT
    ATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTAAATTATAAA
    ACAAATCTTGAACGAAGCAATGAACATGAATATTATGTTGAACATCTAGTCCGCGATGCA
    GTTGGCACACCATTAAAACGTTGTGCTATTGTTAATCGACACAATGGCACAATTATTAAT
    ATTTTTGATGATATGTCAGAAAAAGACAAAGAAGAATTTGAAGCATTTAAAAAGAGAAGC
    CCTAAATACAATCCAGGTATGAACAATCATGATGAAACAGATGGTGAGTCAGAAGACATT
    CAACATCATGACATTGATAATAACAAAGCAATTCAAAATGACATACCAGATCAAAAAGTC
    GACGATAAAAATGATAAAAATGCTGTTAATAAAGAAGAAAAACATGATAATGGGGCAAAT
    AATTCTGAAGAAACTAAAGTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    627

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1848 [new locus tag: SACOL_RS09480 ]
  • symbol: SACOL1848
  • description: hypothetical protein
  • length: 208
  • theoretical pI: 5.39178
  • theoretical MW: 23825.8
  • GRAVY: -1.34567

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 20.2)
  • TheSEED  :
    • Hypothetical protein SAV1801
  • PFAM:
    Cytochrome-c (CL0318) Cytochrom_C; Cytochrome c (PF00034; HMM-score: 9.4)
    LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 9.1)
    and 2 more
    no clan defined DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 6.2)
    Transporter (CL0375) Connexin; Connexin (PF00029; HMM-score: 5.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: SLLTACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 18 aa
    • SP(Sec/SPI): 0.000404
    • TAT(Tat/SPI): 0.000047
    • LIPO(Sec/SPII): 0.999409
    • Cleavage Site: CS pos: 18-19. LTA-CG. Pr: 0.9998
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFKAIVAITLSLSLLTACGANQHKENSSKSNDTNKKTQQTDNTTQSNTEKQMTPQEAEDIVRNDYKARGVNEYQTLNYKTNLERSNEHEYYVEHLVRDAVGTPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMNNHDETDGESEDIQHHDIDNNKAIQNDIPDQKVDDKNDKNAVNKEEKHDNGANNSEETKVK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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