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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1977 [new locus tag: SACOL_RS10335 ]
  • pan locus tag?: SAUPAN004948000
  • symbol: pheA
  • pan gene symbol?: pheA
  • synonym:
  • product: prephenate dehydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1977 [new locus tag: SACOL_RS10335 ]
  • symbol: pheA
  • product: prephenate dehydratase
  • replicon: chromosome
  • strand: +
  • coordinates: 2040724..2041518
  • length: 795
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCAATTATATTATTTAGGCCCTAAAGGTACATTTTCATATTTGGCTTGTCGTCAGTAT
    TTTTCTGAAAATGAAGCAACTTTTCAACCAAAGTCAAATTTTTTTGAAGTCATCAAGGCA
    GTTGCAGATGATGACACATCGATTGGTGTTGTCCCTATTGAAAATTCTATAGAAGGAACA
    ATCAACATTGTGGCAGATGCACTTGCACAGCAAGATGTCTTTGCACACGGTGAAATACGT
    TTAGATATTAACTTTGCATTATATGGTAATGGAACAGATTCCATTTCTGATATTAAAAAA
    GTGTATTCTATTGCACCAGCAATCAGTCAAACAACAAATTACATACATCAACATCAATTT
    GACTATGATTATGTCGACAGTACAATTCAAAGTTTGACTAAAATTGAAAATGGCGTTGCA
    GCAATTGCACCTTTAGGTAGTGGCGAAGCTTATGGTTTTACACCTATTGATACACATATT
    GAAGACTATCCACATAACGTGACACGTTTTTTAGTTATAAAAAATCAGCAGCAATTTGAT
    CAAAATGCGACATCATTGATGTTTTTAATTACACCAATGCATGATAAACCTGGGTTGTTA
    GCAAGTGTGCTTAATACATTTGCTTTGTTTAATATTAATTTATCCTGGATTGAGTCTCGT
    CCTTTAAAAACACAATTAGGTATGTATCGATTTTTCGTTCAGGCTGATTCTGCAATTACA
    ACCGATATAAAGAAAGTCATTGCTATTTTAGAAACCTTAGATTTCAAAGTAGAAATGATT
    GGTGCTTTTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    795

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1977 [new locus tag: SACOL_RS10335 ]
  • symbol: PheA
  • description: prephenate dehydratase
  • length: 264
  • theoretical pI: 4.50653
  • theoretical MW: 29507.2
  • GRAVY: -0.00378788

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.51?  ExPASy
    Prephenate dehydratase Prephenate = phenylpyruvate + H2O + CO2
  • TIGRFAM:
    phenylalanine-4-hydroxylase (TIGR01268; EC 1.14.16.1; HMM-score: 37.1)
  • TheSEED  :
    • Prephenate dehydratase (EC 4.2.1.51)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Chorismate Synthesis  Prephenate dehydratase (EC 4.2.1.51)
    and 2 more
    Amino Acids and Derivatives Aromatic amino acids and derivatives Phenylalanine and Tyrosine Branches from Chorismate  Prephenate dehydratase (EC 4.2.1.51)
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Prephenate dehydratase (EC 4.2.1.51)
  • PFAM:
    PBP (CL0177) PDT; Prephenate dehydratase (PF00800; HMM-score: 145)
    and 2 more
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 28.1)
    no clan defined DUF16; Protein of unknown function DUF16 (PF01519; HMM-score: 17.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012634
    • TAT(Tat/SPI): 0.00035
    • LIPO(Sec/SPII): 0.024323
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADDDTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVYSIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHIEDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIESRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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