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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2070 [new locus tag: SACOL_RS10830 ]
  • pan locus tag?: SAUPAN005351000
  • symbol: kdpD
  • pan gene symbol?: kdpD
  • synonym:
  • product: sensor histidine kinase KdpD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2070 [new locus tag: SACOL_RS10830 ]
  • symbol: kdpD
  • product: sensor histidine kinase KdpD
  • replicon: chromosome
  • strand: +
  • coordinates: 2133722..2136379
  • length: 2658
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTCAAACACTGAATCGCTAAACATAGGAAAAAAGCGTGGATCTTTAACAATTTACATC
    GGTTACAGTCCAGGTGTTGGTAAAACATTTGAGATGCTTTCAAATGCCATTGAACTATTT
    CAAAGTAACGTTGATATTAAAATAGGATATATTGAACCGCATCAGCGTGATGAAACAAAT
    GCATTAGCTGAACAATTACCTAAAATCACAACCAATTTTACTAAACATGGTAGTCATCAT
    TTTCAATATTTAGATGTCGACCGCATAATCGAAGAATCGCCGACAATAGTACTTATCGAT
    GAGTTAGCACATACGAATATTTCTAGAGATCGTCATGAGAAACGATATATGGATATTGAA
    GAAATTTTAAATCATGGTATCGATGTTCATACCACTTTGAACATTCAACATATTGAAAGT
    TTAAGTAGTCAAATTGAACTGATGACCGGTGTACATGTTAAAGAACGTGTACCCGACTAT
    TTCATAATGAGCGCCGATGTATTAGAAGTCGTAGATATCTCACCTGAACAATTAATTAAA
    CGCTTAAAAGCTGGCAAGGTATATAAAAAGGATAGGCTAGATGTAGCATTTAGTAATTTC
    TTTACGTATGCCCACCTAAGCGAACTGCGTACATTGACGTTAAGAACAGTTGCCGACTTG
    ATGAGTGATAAAGAAAAAGTCCGACACAACCATAAAACGTCACTCAAACCTCATATTGCT
    GTGGCAATTAGTGGGAGCATTTATAATGAAGCAGTAATTAAAGAGGCATTCCATATTGCT
    CAAAAAGAACATGCGAAGTTCACTGCTATTTATATAGATGTATTCGAAAAAAACAGGCAA
    TATAAAGATAGTCAAAAGCAAGTGCATCAACATCTCATGCTTGCAAAATCATTAGGAGCA
    AAAGTAAAAGTAGTTTATAGCCAAACCGTTGCATTAGGATTAGACGAATGGTGTAAAAAT
    CAAGATGTAACCAAATTAATTATCGGACAACATATTAGAAATAAGTGGCGAGACTTTTTC
    AATACACCTTTAATTGACCATTTAATGTCCTTTGAACATAGCTATAAAATCGAAATCGTT
    CCAATCAAACAAATACCTGTTGAATTGAAAATGAACAAATCACCCTATCGTCCTAAAGGC
    AAACGTTTCGCCATAGATATGTTAAAAATGATTTTGATTCAAATAATTTGTGTAATGATG
    GGACTGTGGATTTATCAACTTGATAAGCATGAGTCTAGTACGATTATTTTAATGATTTTT
    CTCATCGGCATCATTTTATTATCCATTTGGACGCGGTCCTTCATCATTGGCTTTTTAGCA
    GCAATTATTAACGTATTTGTTTTTAATTATTTTTTTACGGAACCTAGATATACATTTGAA
    GTATATCGCTTTGACTATCCTATTACATTTATCGTTAGCATTTTAACAAGTATTTTAACG
    AGTGCTTTATTAAAACAAATTAAATTCCAATACTCCATTACTAAAAAGCAACTTTATCGT
    ACAGATTTATTATTTCAATTTAATGATTCGATTAAACAAACTTATACAGTTGAAAACTTA
    CTAATAAATGCTGGATACCAAATTAATCAATTATTGCAACAATCAATTACTATATACGTT
    ATCAATCAATCGAAAGTAATTAAAACAATACCATTGCAAAACCATATCGATAATACGACT
    CAACAGCATGAACAAGCATTAAGCTGGGTAATTAAAAATGAAAGACAAGCTGGTGCAACA
    ACAGATACATTTCCAGGCATTAATAAATGGTTGATACCAATTGGCACATCTCCGATTAAA
    GGCATTCTAGCCATTGATTACCAAAGTTCTCAAGTGATTAATCCATATGATGCATCAATA
    TTAGAATCAATGTTAAATGAACTTTCTCTCGCCGTTGAAAATGTGACGTTACTTAAACAA
    ACGAGAGAATCCATGTTACAGGCTGAACGTCAACTGACACATTCAAATTTTTTAAGATCA
    ATTTCGCATGACATACGTACACCGTTAACAACAATTATGGGAAATTTAGATATTTTAGTA
    TCACATAGTAAAGATATGTCTGTCATAGAAAAGGAACAATTACTTGTACATAGTTTTCAA
    GAAAGTCAGTATTTATATCTATTAGTGACAAATATTTTATCATTAACAAAATTACAGTCA
    TCAAATGTTCAGATAAAATTGCAACCTTATCTCGTTAGTGAATTAGTAGAAGAAATCGAT
    ATGATTTTAGAACGTCGCCATTTAAAAAAGCGTATAACTGTATCATCCTCAGTAAATTTA
    CAGTTTATACACATTGACAGTAAGTTGATTTTACAAGCTTTATTCAATTTGATTGAAAAT
    GCAGTTAAACACACTTCTACTGACACCAAAATCAATTTATCTATTCGTTATGCTAGCTAT
    GAACAAATCGAATTTGCCGTTATAGACGAAGGACCTGGAATTAGTTTAGAAGAACAACAA
    AAGATATTCGAGCCTTTTTACACAGGATCAAATAAATATTTTAAAGATAACCAAAAAGAA
    AGCATGGGACTAGGCTTATATTTAGTACAGACTATCCTACACAAACATCAATCAAACTTA
    CAATATAAACCGAACCAACCACACGGCAGTATATTCTATTTCAATATATATACAGACTTT
    AACGAAGGAGACGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2658

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2070 [new locus tag: SACOL_RS10830 ]
  • symbol: KdpD
  • description: sensor histidine kinase KdpD
  • length: 885
  • theoretical pI: 7.78455
  • theoretical MW: 102204
  • GRAVY: -0.140678

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 118)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 107.8)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 99.8)
    and 4 more
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 34.4)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 34.4)
    anti-sigma B factor (TIGR01924; EC 2.7.11.1; HMM-score: 24)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutL (TIGR00585; HMM-score: 16.3)
  • TheSEED  :
    • Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)
    Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)
  • PFAM:
    P-loop_NTPase (CL0023) KdpD; Osmosensitive K+ channel His kinase sensor domain (PF02702; HMM-score: 263.3)
    and 9 more
    no clan defined DUF4118; Domain of unknown function (DUF4118) (PF13493; HMM-score: 82.8)
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 61.5)
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 34.5)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 19.4)
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 18.9)
    Phosphatase (CL0031) Y_phosphatase3; Tyrosine phosphatase family (PF13350; HMM-score: 15.8)
    HUP (CL0039) Usp; Universal stress protein family (PF00582; HMM-score: 15.1)
    no clan defined TctA; Tripartite tricarboxylate transporter TctA family (PF01970; HMM-score: 12.9)
    Holin-V (CL0562) Phage_holin_5_2; Phage holin family Hol44, in holin superfamily V (PF16079; HMM-score: 8.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005666
    • TAT(Tat/SPI): 0.000411
    • LIPO(Sec/SPII): 0.000706
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNVDIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDELAHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDYFIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVADLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFEKNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRNKWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMILIQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNYFFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFNDSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQALSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESMLNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSHSKDMSVIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEIDMILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYASYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKHQSNLQYKPNQPHGSIFYFNIYTDFNEGDV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Ting Xue, Yibo You, De Hong, Haipeng Sun, Baolin Sun
The Staphylococcus aureus KdpDE two-component system couples extracellular K+ sensing and Agr signaling to infection programming.
Infect Immun: 2011, 79(6);2154-67
[PubMed:21422185] [WorldCat.org] [DOI] (I p)

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