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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2088 [new locus tag: SACOL_RS10925 ]
- pan locus tag?: SAUPAN005381000
- symbol: SACOL2088
- pan gene symbol?: sceD
- synonym:
- product: sceD protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2088 [new locus tag: SACOL_RS10925 ]
- symbol: SACOL2088
- product: sceD protein
- replicon: chromosome
- strand: -
- coordinates: 2151898..2152593
- length: 696
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238684 NCBI
- RefSeq: YP_186903 NCBI
- BioCyc: see SACOL_RS10925
- MicrobesOnline: 913564 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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661ATGAAGAAAACATTACTCGCATCATCATTAGCAGTAGGTTTAGGAATCGTAGCAGGAAAT
GCAGGTCACGAAGCCCATGCAAGTGAAGCGGACTTAAATAAAGCATCTTTAGCGCAAATG
GCGCAATCAAATGATCAAACATTAAATCAAAAACCAATTGAAGCTGGGGCTTATAATTAT
ACATTTGACTATGAAGGGTTTACTTATCACTTTGAATCAGATGGTACACACTTTGCTTGG
AATTACCATGCAACAGGTACTAATGGAGCAGACATGAGTGCACAAGCACCTACAACTAAT
AATGTTGCACCATCAGCTGTTCAAGCTAATCAAGTACAATCACAAGAAGTTGAAGCACCA
CAAAATGCTCAAACTCAACAACCACAAGCATCAACATCAAACAATTCACAAGTTACTGCA
ACACCAACTGAATCAAAATCATCAGAAGGTTCATCAGTAAATGTGAATGCTCATCTAAAA
CAAATTGCTCAACGTGAATCAGGTGGCAATATTCATGCTGTAAATCCAACATCAGGTGCA
GCTGGTAAGTATCAATTCTTACAATCAACTTGGGATTCAGTAGCACCTGCTAAATATAAA
GGTGTATCACCAGCAAATGCTCCTGAAAGTGTTCAAGATGCCGCAGCAGTAAAATTATAT
AACACTGGTGGCGCTGGACATTGGGTTACTGCATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2088 [new locus tag: SACOL_RS10925 ]
- symbol: SACOL2088
- description: sceD protein
- length: 231
- theoretical pI: 5.79248
- theoretical MW: 24095.9
- GRAVY: -0.557576
⊟Function[edit | edit source]
- reaction: EC 3.2.-.-? ExPASy
- TIGRFAM:
- TheSEED :
- SceD-like transglycosylase, biomarker for vancomycin-intermediate strains
- PFAM: Lysozyme (CL0037) Transglycosylas; Transglycosylase-like domain (PF06737; HMM-score: 95.7)and 2 moreSLT; Transglycosylase SLT domain (PF01464; HMM-score: 22.5)no clan defined GET2; GET complex subunit GET2 (PF08690; HMM-score: 5.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.02
- Extracellular Score: 9.98
- Internal Helices: 0
- LocateP: Secretory(released) (with CS)
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: -0.17
- Signal peptide possibility: 1
- N-terminally Anchored Score: -2
- Predicted Cleavage Site: HEAHASEA
- SignalP: Signal peptide SP(Sec/SPI) length 27 aa
- SP(Sec/SPI): 0.986312
- TAT(Tat/SPI): 0.003284
- LIPO(Sec/SPII): 0.008779
- Cleavage Site: CS pos: 27-28. AHA-SE. Pr: 0.9175
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSNDQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPTTNNVAPSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLKQIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVKLYNTGGAGHWVTA
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Signal peptide containing [1] [2] [3] [4]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- data available for NCTC8325
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
A K Ziebandt, H Weber, J Rudolph, R Schmid, D Höper, S Engelmann, M Hecker
Extracellular proteins of Staphylococcus aureus and the role of SarA and sigma B.
Proteomics: 2001, 1(4);480-93
[PubMed:11681202] [WorldCat.org] [DOI] (P p)