From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02333
  • pan locus tag?: SAUPAN005381000
  • symbol: SAOUHSC_02333
  • pan gene symbol?: sceD
  • synonym:
  • product: transglycosylase SceD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02333
  • symbol: SAOUHSC_02333
  • product: transglycosylase SceD
  • replicon: chromosome
  • strand: -
  • coordinates: 2162622..2163317
  • length: 696
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGAAAACATTACTCGCATCATCATTAGCAGTAGGTTTAGGAATCGTAGCAGGAAAT
    GCAGGTCACGAAGCCCATGCAAGTGAAGCGGACTTAAATAAAGCATCTTTAGCGCAAATG
    GCGCAATCAAATGATCAAACATTAAATCAAAAACCAATTGAAGCTGGGGCTTATAATTAT
    ACATTTGACTATGAAGGGTTTACTTATCACTTTGAATCAGATGGTACACACTTTGCTTGG
    AATTACCATGCAACAGGTACTAATGGAGCAGACATGAGTGCACAAGCACCTGCAACTAAT
    AATGTTGCACCATCAGCTGTTCAAGCTAATCAAGTACAATCACAAGAAGTTGAAGCACCA
    CAAAATGCTCAAACTCAACAACCACAAGCATCAACATCAAACAATTCACAAGTTACTGCA
    ACACCAACTGAATCAAAATCATCAGAAGGTTCATCAGTAAATGTGAATGCTCATCTAAAA
    CAAATTGCTCAACGTGAATCAGGTGGCAATATTCATGCTGTAAATCCAACATCAGGTGCA
    GCTGGTAAGTATCAATTCTTACAATCAACTTGGGATTCAGTAGCACCTGCTAAATATAAA
    GGTGTATCACCAGCAAATGCTCCTGAAAGTGTTCAAGATGCCGCAGCAGTAAAATTATAT
    AACACTGGTGGCGCTGGACATTGGGTTACTGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    696

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02333
  • symbol: SAOUHSC_02333
  • description: transglycosylase SceD
  • length: 231
  • theoretical pI: 5.79248
  • theoretical MW: 24065.9
  • GRAVY: -0.546753

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.-.-?  ExPASy
  • TIGRFAM:
  • TheSEED  :
    • SceD-like transglycosylase, biomarker for vancomycin-intermediate strains
  • PFAM:
    Lysozyme (CL0037) Transglycosylas; Transglycosylase-like domain (PF06737; HMM-score: 95.7)
    and 1 more
    SLT; Transglycosylase SLT domain (PF01464; HMM-score: 22.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: HEAHASEA
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.986312
    • TAT(Tat/SPI): 0.003284
    • LIPO(Sec/SPII): 0.008779
    • Cleavage Site: CS pos: 27-28. AHA-SE. Pr: 0.9175
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSNDQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNVAPSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLKQIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVKLYNTGGAGHWVTA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: WalR* (activation) regulon
    WalR*(response regulator)important in cell wall homeostasis;  [3]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Sarah Dubrac, Ivo Gomperts Boneca, Olivier Poupel, Tarek Msadek
    New insights into the WalK/WalR (YycG/YycF) essential signal transduction pathway reveal a major role in controlling cell wall metabolism and biofilm formation in Staphylococcus aureus.
    J Bacteriol: 2007, 189(22);8257-69
    [PubMed:17827301] [WorldCat.org] [DOI] (I p)
  4. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Melanie R Stapleton, Malcolm J Horsburgh, Emma J Hayhurst, Lynda Wright, Ing-Marie Jonsson, Andrej Tarkowski, John F Kokai-Kun, James J Mond, Simon J Foster
Characterization of IsaA and SceD, two putative lytic transglycosylases of Staphylococcus aureus.
J Bacteriol: 2007, 189(20);7316-25
[PubMed:17675373] [WorldCat.org] [DOI] (P p)

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_02333&oldid=85740"