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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2154 [new locus tag: SACOL_RS11275 ]
- pan locus tag?: SAUPAN005509000
- symbol: rocF
- pan gene symbol?: rocF
- synonym:
- product: arginase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2154 [new locus tag: SACOL_RS11275 ]
- symbol: rocF
- product: arginase
- replicon: chromosome
- strand: -
- coordinates: 2225416..2226324
- length: 909
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237431 NCBI
- RefSeq: YP_186967 NCBI
- BioCyc: see SACOL_RS11275
- MicrobesOnline: 913630 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGACAAAGACAAAAGCAATTGATATTATAGGTGCACCATCAACATTTGGACAAAGAAAA
TTAGGTGTTGATTTAGGACCAACAGCAATTAGATATGCTGGATTAATTTCAAGATTAAAG
CAATTAGACCTTGATGTATATGACAAGGGGGATATTAAGGTACCTGCTGTGAACATTGAA
AAATTTCATAGTGAACAAAAAGGATTAAGAAATTATGATGAAATTATAGATGTTAATCAA
AAATTAAATAAAGAGGTTTCAGCATCAATTGAAAATAACAGATTTCCTCTAGTTCTTGGT
GGAGATCATTCTATTGCGGTAGGTTCAGTATCAGCAATAAGTAAACATTATAATAATTTA
GGTGTTATTTGGTATGATGCACATGGTGATTTAAATATACCTGAAGAGTCACCAAGTGGA
AATATTCATGGTATGCCTCTAAGGATTTTGACAGGCGAAGGTCCCAAAGAACTTTTAGAA
TTAAATAGTAATGTAATCAAGCCAGAAAACATCGTACTAATTGGTATGAGAGATTTAGAT
AAAGGTGAAAGACAATTTATCAAAGATCATAATATTAAAACATTTACTATGTCAGATATT
GATAAATTGGGGATAAAGGAAGTAATTGAAAATACAATAGAATATTTGAAGTCACGCAAT
GTTGATGGCGTTCATTTATCTTTAGATGTTGATGCTTTAGATCCGCTTGAAACGCCAGGC
ACTGGTACTAGAGTTTTGGGTGGTCTTAGTTATAGAGAAAGCCATTTTGCATTGGAATTA
CTGCATCAATCACATTTAATTTCCTCAATGGATTTAGTTGAAGTAAATCCATTGATTGAC
AGTAATAATCATACTGCTGAACAAGCGGTTTCATTAGTTGGAACATTTTTTGGTGAAACT
TTATTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2154 [new locus tag: SACOL_RS11275 ]
- symbol: RocF
- description: arginase
- length: 302
- theoretical pI: 5.21395
- theoretical MW: 33264.6
- GRAVY: -0.216887
⊟Function[edit | edit source]
- reaction: EC 3.5.3.1? ExPASyArginase L-arginine + H2O = L-ornithine + urea
- TIGRFAM: arginase (TIGR01229; EC 3.5.3.1; HMM-score: 329.6)and 2 moreagmatinase (TIGR01230; EC 3.5.3.11; HMM-score: 108)Energy metabolism Amino acids and amines formimidoylglutamase (TIGR01227; EC 3.5.3.8; HMM-score: 98.8)
- TheSEED :
- Arginase (EC 3.5.3.1)
- PFAM: Arginase (CL0302) Arginase; Arginase family (PF00491; HMM-score: 258.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Mn2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003747
- TAT(Tat/SPI): 0.000601
- LIPO(Sec/SPII): 0.000629
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDLDVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGDHSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLELNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKSRNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVNPLIDSNNHTAEQAVSLVGTFFGETLL
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: CcpA regulon
CcpA (TF) important in Carbon catabolism; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
S W Wu, H De Lencastre
Mrp--a new auxiliary gene essential for optimal expression of methicillin resistance in Staphylococcus aureus.
Microb Drug Resist: 1999, 5(1);9-18
[PubMed:10332717] [WorldCat.org] [DOI] (P p)