From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2065 [new locus tag: NWMN_RS11920 ]
  • pan locus tag?: SAUPAN005509000
  • symbol: NWMN_2065
  • pan gene symbol?: rocF
  • synonym:
  • product: arginase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2065 [new locus tag: NWMN_RS11920 ]
  • symbol: NWMN_2065
  • product: arginase
  • replicon: chromosome
  • strand: -
  • coordinates: 2293799..2294707
  • length: 909
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACAAAGACAAAAGCAATTGATATTATAGGTGCACCATCAACATTTGGACAAAGAAAA
    TTAGGTGTTGATTTAGGACCAACAGCAATTAGATATGCTGGATTAATTTCAAGATTAAAG
    CAATTAGACCTTGATGTATATGACAAGGGGGATATTAAGGTACCTGCTGTGAACATTGAA
    AAATTTCATAGTGAACAAAAAGGATTAAGAAATTATGATGAAATTATAGATGTTAATCAA
    AAATTAAATAAAGAGGTTTCAGCATCAATTGAAAATAACAGATTTCCTCTAGTTCTTGGT
    GGAGATCATTCTATTGCGGTAGGTTCAGTATCAGCAATAAGTAAACATTATAATAATTTA
    GGTGTTATTTGGTATGATGCACATGGTGATTTAAATATACCTGAAGAGTCACCAAGTGGA
    AATATTCATGGTATGCCTCTAAGGATTTTGACAGGCGAAGGTCCCAAAGAACTTTTAGAA
    TTAAATAGTAATGTAATCAAGCCAGAAAACATCGTACTAATTGGTATGAGAGATTTAGAT
    AAAGGTGAAAGACAATTTATCAAAGATCATAATATTAAAACATTTACTATGTCAGATATT
    GATAAATTGGGGATAAAGGAAGTAATTGAAAATACAATAGAATATTTGAAGTCACGCAAT
    GTTGATGGCGTTCATTTATCTTTAGATGTTGATGCTTTAGATCCGCTTGAAACGCCAGGC
    ACTGGTACTAGAGTTTTGGGTGGTCTTAGTTATAGAGAAAGCCATTTTGCATTGGAATTA
    CTGCATCAATCACATTTAATTTCCTCAATGGATTTAGTTGAAGTAAATCCATTGATTGAC
    AGTAATAATCATACTGCTGAACAAGCGGTTTCATTAGTTGGAACATTTTTTGGTGAAACT
    TTATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    909

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2065 [new locus tag: NWMN_RS11920 ]
  • symbol: NWMN_2065
  • description: arginase
  • length: 302
  • theoretical pI: 5.21395
  • theoretical MW: 33264.6
  • GRAVY: -0.216887

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.3.1?  ExPASy
    Arginase L-arginine + H2O = L-ornithine + urea
  • TIGRFAM:
    arginase (TIGR01229; EC 3.5.3.1; HMM-score: 329.6)
    and 2 more
    agmatinase (TIGR01230; EC 3.5.3.11; HMM-score: 108)
    Metabolism Energy metabolism Amino acids and amines formimidoylglutamase (TIGR01227; EC 3.5.3.8; HMM-score: 98.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Arginase (CL0302) Arginase; Arginase family (PF00491; HMM-score: 258.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003747
    • TAT(Tat/SPI): 0.000601
    • LIPO(Sec/SPII): 0.000629
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDLDVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGDHSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLELNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKSRNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVNPLIDSNNHTAEQAVSLVGTFFGETLL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_2065&oldid=69783"