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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2179 [new locus tag: SACOL_RS11465 ]
  • pan locus tag?: SAUPAN005584000
  • symbol: SACOL2179
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2179 [new locus tag: SACOL_RS11465 ]
  • symbol: SACOL2179
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2261369..2262238
  • length: 870
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGAAGAAAATTTTTGTAATTATTACGACATTGTTAGCGGTCGCTATTATTATAGGCTCT
    ATTATTATGGTGGTGTTTAGTCAACGACAGGCACAAACATTTAAAATACAACAACAGCAA
    TTTGTTAAAAAGCCAATTCCAACATTGTTCTTACATGGATTTGGTGGTAGTGCCAATTCT
    GAAAAGTTTATGGTGAAGCAAGCAGAAAAAAGAGGGGTAACTAAAGATATCATCACTGCT
    TACGTTTCAAAGGATGGTGCAGTGACATTTAAAGGGAAACTAAGAAAAGATGCAGTGAAT
    CCTATTGTGAAAATTGAACTTGAAAATAACAGACAAGGCTATTTGGATAAGAATGCTGCG
    TGGTTTAAAAATGTACTGACTAAGTTACAATCTGAATACAATTTCGATAAATTCAACTTT
    GTCGGTCACTCAATGGGGAACTTAACATTTGCACAATATATGATGACATATGGTAACGAC
    AAGTCACTGCCACAGTTAAACAAACAAGTGAATATTGCAGGGACATTTAATGGTGTTTTA
    AATATGAATGAAGATGTCAATGAAATTACAGTTGATAAAGATGGTAAACCAAGTCGAATG
    AATCAACCTTATCAGCAATTACGCGTGCTAAAAGATATTTATAAAGGTAAAGGTATCGAA
    GTGCTAAATATTTATGGGGATTTGAAAGATGGTACACATTCAGACGGCAGAGTGTCTAAT
    AGTTCTTCGAAATCATTAAAATATTTACTGGGAAATAGTCCGAAAAGTTATCGAGAATCT
    AAATATGAAGGTGAGCCTGCACAACATAGTCAGCTTCATGAGAATGAAAATTTTGCTAAT
    GAGTTAATCGACTTCTTATGGAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    870

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2179 [new locus tag: SACOL_RS11465 ]
  • symbol: SACOL2179
  • description: hypothetical protein
  • length: 289
  • theoretical pI: 10.1106
  • theoretical MW: 32763.4
  • GRAVY: -0.461246

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • cell surface hydrolase (putative)
  • PFAM:
    AB_hydrolase (CL0028) DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 340.6)
    and 5 more
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 16.1)
    DUF676; Putative serine esterase (DUF676) (PF05057; HMM-score: 15.7)
    no clan defined DAG1; Dystroglycan (Dystrophin-associated glycoprotein 1) (PF05454; HMM-score: 13.4)
    AB_hydrolase (CL0028) DUF900; Alpha/beta hydrolase of unknown function (DUF900) (PF05990; HMM-score: 12.1)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: IQQQQFVK
  • SignalP: Signal peptide SP(Sec/SPI) length 31 aa
    • SP(Sec/SPI): 0.56263
    • TAT(Tat/SPI): 0.001715
    • LIPO(Sec/SPII): 0.027241
    • Cleavage Site: CS pos: 31-32. RQA-QT. Pr: 0.3392
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKKPIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPIVKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGNDKSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKGIEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENENFANELIDFLWKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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