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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2148 [new locus tag: SAUSA300_RS11835 ]
  • pan locus tag?: SAUPAN005584000
  • symbol: SAUSA300_2148
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2148 [new locus tag: SAUSA300_RS11835 ]
  • symbol: SAUSA300_2148
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2325236..2326105
  • length: 870
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGAAGAAAATTTTTGTAATTATTACGACATTGTTAGCGGTCGCTATTATTATAGGCTCT
    ATTATTATGGTGGTGTTTAGTCAACGACAGGCACAAACATTTAAAATACAACAACAGCAA
    TTTGTTAAAAAGCCAATTCCAACATTGTTCTTACATGGATTTGGTGGTAGTGCCAATTCT
    GAAAAGTTTATGGTGAAGCAAGCAGAAAAAAGAGGGGTAACTAAAGATATCATCACTGCT
    TACGTTTCAAAGGATGGTGCAGTGACATTTAAAGGGAAACTAAGAAAAGATGCAGTGAAT
    CCTATTGTGAAAATTGAACTTGAAAATAACAGACAAGGCTATTTGGATAAGAATGCTGCG
    TGGTTTAAAAATGTACTGACTAAGTTACAATCTGAATACAATTTCGATAAATTCAACTTT
    GTCGGTCACTCAATGGGGAACTTAACATTTGCACAATATATGATGACATATGGTAACGAC
    AAGTCACTGCCACAGTTAAACAAACAAGTGAATATTGCAGGGACATTTAATGGTGTTTTA
    AATATGAATGAAGATGTCAATGAAATTACAGTTGATAAAGATGGTAAACCAAGTCGAATG
    AATCAACCTTATCAGCAATTACGCGTGCTAAAAGATATTTATAAAGGTAAAGGTATCGAA
    GTGCTAAATATTTATGGGGATTTGAAAGATGGTACACATTCAGACGGCAGAGTGTCTAAT
    AGTTCTTCGAAATCATTAAAATATTTACTGGGAAATAGTCCGAAAAGTTATCGAGAATCT
    AAATATGAAGGTGAGCCTGCACAACATAGTCAGCTTCATGAGAATGAAAATGTTGCTAAT
    GAGTTAATCGACTTCTTATGGAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    870

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2148 [new locus tag: SAUSA300_RS11835 ]
  • symbol: SAUSA300_2148
  • description: hypothetical protein
  • length: 289
  • theoretical pI: 10.1106
  • theoretical MW: 32715.3
  • GRAVY: -0.456401

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • cell surface hydrolase (putative)
  • PFAM:
    AB_hydrolase (CL0028) DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 344.2)
    and 5 more
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 16.1)
    DUF676; Putative serine esterase (DUF676) (PF05057; HMM-score: 15.8)
    no clan defined DAG1; Dystroglycan (Dystrophin-associated glycoprotein 1) (PF05454; HMM-score: 13.4)
    AB_hydrolase (CL0028) DUF900; Alpha/beta hydrolase of unknown function (DUF900) (PF05990; HMM-score: 12.1)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: IQQQQFVK
  • SignalP: Signal peptide SP(Sec/SPI) length 31 aa
    • SP(Sec/SPI): 0.56263
    • TAT(Tat/SPI): 0.001715
    • LIPO(Sec/SPII): 0.027241
    • Cleavage Site: CS pos: 31-32. RQA-QT. Pr: 0.3392
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKKPIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPIVKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGNDKSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKGIEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENENVANELIDFLWKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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