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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2185
  • pan locus tag?: SAUPAN005591000
  • symbol: lacB
  • pan gene symbol?: lacB
  • synonym:
  • product: galactose-6-phosphate isomerase subunit LacB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2185
  • symbol: lacB
  • product: galactose-6-phosphate isomerase subunit LacB
  • replicon: chromosome
  • strand: -
  • coordinates: 2267914..2268429
  • length: 516
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAGATTGCATTAGGATGCGACCATATTGTTACAGATACAAAAATGCGTGTATCTGAA
    TTTTTAAAATCAAAAGGACATGAAGTCATTGACGTAGGAACATACGATTTCACAAGAACA
    CATTATCCAATTTTTGGTAAAAAAGTTGGCGAACAAGTTGTTAGCGGTAATGCAGACTTA
    GGTGTTTGTATTTGTGGAACAGGTGTTGGTATTAACAATGCTGTAAATAAAGTACCTGGC
    GTTCGTTCAGCACTAGTACGTGATATGACATCAGCGTTATACGCTAAAGAAGAATTAAAT
    GCGAACGTTATTGGCTTCGGTGGACGTATTATAGGTGAGTTATTAATGTGCGATATTATC
    GATGCATTTATTAATGCTGAATATAAACCAACTGAAGAGAACAAAAAATTAATCGCTAAA
    ATTAAACATTTAGAAACAAGCAATGCAGATCAAGCTGATCCACATTTCTTTGATGAATTC
    TTAGAAAAATGGGACAGAGGCGAATACCACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    516

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2185
  • symbol: LacB
  • description: galactose-6-phosphate isomerase subunit LacB
  • length: 171
  • theoretical pI: 5.59496
  • theoretical MW: 18950.5
  • GRAVY: -0.212866

⊟Function[edit | edit source]

  • reaction:
    EC 5.3.1.26?  ExPASy
    Galactose-6-phosphate isomerase D-galactose 6-phosphate = D-tagatose 6-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides galactose-6-phosphate isomerase, LacB subunit (TIGR01119; EC 5.3.1.26; HMM-score: 375.9)
    and 5 more
    sugar-phosphate isomerase, RpiB/LacA/LacB family (TIGR00689; EC 5.3.1.-; HMM-score: 124.7)
    Metabolism Energy metabolism Pentose phosphate pathway ribose 5-phosphate isomerase B (TIGR01120; EC 5.3.1.6; HMM-score: 117.9)
    ribose 5-phosphate isomerase (TIGR02133; EC 5.3.1.6; HMM-score: 106)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides galactose-6-phosphate isomerase, LacA subunit (TIGR01118; EC 5.3.1.26; HMM-score: 48.8)
    Hypothetical proteins Conserved conserved hypothetical protein EF_0830/AHA_3911 (TIGR03577; HMM-score: 13.5)
  • TheSEED  :
    • Galactose-6-phosphate isomerase, LacB subunit (EC 5.3.1.26)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Galactose-6-phosphate isomerase, LacB subunit (EC 5.3.1.26)
  • PFAM:
    no clan defined LacAB_rpiB; Ribose/Galactose Isomerase (PF02502; HMM-score: 142.9)
    and 3 more
    Gly_rich_SFCGS; Glycine-rich SFCGS (PF14272; HMM-score: 16.5)
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 13.3)
    no clan defined Ku_PK_bind; Ku C terminal domain like (PF08785; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.05171
    • TAT(Tat/SPI): 0.000494
    • LIPO(Sec/SPII): 0.002331
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPIFGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNANVIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEFLEKWDRGEYHD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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