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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2303 [new locus tag: SACOL_RS12115 ]
  • pan locus tag?: SAUPAN005789000
  • symbol: SACOL2303
  • pan gene symbol?: —
  • synonym:
  • product: inositol monophosphatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2303 [new locus tag: SACOL_RS12115 ]
  • symbol: SACOL2303
  • product: inositol monophosphatase
  • replicon: chromosome
  • strand: -
  • coordinates: 2365762..2366559
  • length: 798
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACAGATAAAACATTACAGCAAATAGATAAGTTAATATGTTCGTGGTTGAAACAAATA
    GACAATGTTATTCCGCAATTAATTATGGAAATGACTACAGAAACTAAGCGTCATCGATTT
    GATTTAGTTACAAATGTAGATAAACAGATTCAACAACAATTCCAACAATTTTTAGCAACA
    CATTTTCCGGAACACCAACTATTAGCGGAAGAAAAAAGTAATGAAATGATTACGAATGAA
    ATTAATCATTTATGGATTATGGATCCCATTGATGGAACTGCCAATTTAGTAAAACAGCAA
    GAAGATTATTGTATTATATTGGCGTATTTTTATGAAGGTAAACCAATGTTATCGTACGTA
    TATGATTATCCGCATAAAAAGCTTTATAAAGCAATACGAGGAGAAGGTGCTTTTTGTAAT
    GGAATTAAGATGGAAGAACCACCATCGTTAAAATTGGAAGACGCTATTATATCGTTTAAT
    GCACAAGTGATGAATCTAGATACGGTGCAAGATTTATTTGACGCCTCATTTAGTTATCGT
    TTAGTTGGTGCTTGCGGATTAGATTCTATGCGAGTGGCAAAAGGTCAATTTGGGGCACAT
    ATTAATACAAATCCTAAACCTTGGGACATTGCTGCACAGTTTTTATTTGCAGAACTATTG
    AATCTAAAAATGACGACTTTAGATGGTAAGGCAATTGATCATTTAAAGGGTGCACCTTTT
    ATTATAAGTAATAAAGCATGTCATGAAACGGTACTTAAAATTTTAAATGCAAATGGTGGT
    TATCAAAAATACAGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    798

⊟Protein[edit | edit source]

Protein Data Bank: 5EYG
Protein Data Bank: 5EYH
Protein Data Bank: 5F24

⊟General[edit | edit source]

  • locus tag: SACOL2303 [new locus tag: SACOL_RS12115 ]
  • symbol: SACOL2303
  • description: inositol monophosphatase
  • length: 265
  • theoretical pI: 6.30614
  • theoretical MW: 30384.9
  • GRAVY: -0.264906

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphatase (TIGR02067; EC 3.1.3.15; HMM-score: 89.8)
    and 3 more
    Metabolism Central intermediary metabolism Sulfur metabolism 3'(2'),5'-bisphosphate nucleotidase (TIGR01331; EC 3.1.3.7; HMM-score: 57.8)
    3'(2'),5'-bisphosphate nucleotidase (TIGR01330; EC 3.1.3.7; HMM-score: 24.4)
    TIGR03545 family protein (TIGR03545; HMM-score: 11.2)
  • TheSEED  :
    • Inositol-1-monophosphatase-like protein
  • PFAM:
    Phospoesterase (CL0171) Inositol_P; Inositol monophosphatase family (PF00459; HMM-score: 125.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001541
    • TAT(Tat/SPI): 0.000103
    • LIPO(Sec/SPII): 0.000337
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVTNVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQEDYCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFNAQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAELLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [3]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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