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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL
locus tag: SACOL2328 [new locus tag: SACOL_RS12240 ]
pan locus tag^?: SAUPAN005823000
symbol: SACOL2328
pan gene symbol^?: lyrA
synonym:
product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL2328 [new locus tag: SACOL_RS12240 ]
symbol: SACOL2328
product: hypothetical protein
replicon: chromosome
strand: +
coordinates: 2390766..2392025
length: 1260
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID: 3238443 NCBI
RefSeq: YP_187135 NCBI
BioCyc: see SACOL_RS12240
MicrobesOnline: 913810 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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ATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTC
ATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACAC
TTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTT
TTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATT
GAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCTAATTATTGGTATGTACAGC
TTTAATACATTTGCAGATAGCTTTATTTTATTACAATCAACAGGCTTATCAGTACCTATT
ACACACATTCTGATTGGACATATTCTGATGGCGTTCGTAGTAGAATTCGGATTCCGTTCA
TACTTACAAAATATTGTTGAAACGAAAATGAACACGTTCTTTGCAAGTATTGTTGTTGGT
TTGATGTATTCAGTTTTCTCAGCAAATACAACTTATGGTACAGAATTTGCTGCTTATAAC
TTCCTTTATACATTCTCATTCTCTATGATTCTTGGTGAATTAATTAGAGCGACTAAAGGA
CGTACAATTTATATTGCAACGACATTCCATGCTTCAATGACATTCGGACTTATTTTCTTG
TTTAGCGAAGAAATCGGCGATCTATTTTCAATCAAAGTCATCGCCATTTCAACAGCAATC
GTTGCAGTAGGATACATTGGTTTAAGCTTAATTATCCGAGGTATTGCATATTTAACAACA
AGACGAAACCTTGAAGAACTTGAGCCTAATAATTATTTAGACCATGTCAATGACGATGAA
GAAACTAATCATACTGAGGCTGAAAAATCTTCTTCAAATATTAAAGATGCTGAAAAAACA
GGTGTAGCTACTGCATCAACGGTTGGTGTTGCTAAAAATGATACTGAAAATACAGTGGCT
GACGAACCAAGCATTCATGAAGGTACTGAAAAAACAGAACCTCAACATCACATAGGTAAT
CAAACTGAATCTAATCATGATGAAGATCATGACATCACTTCGGAGTCAGTAGAATCAGCA
GAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAA
ATAGAGCAATCATTAAAAGAACCTGTGACTTATAAAGAAGACAGACGTTCATCAGTTGTA
ATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SACOL2328 [new locus tag: SACOL_RS12240 ]
symbol: SACOL2328
description: hypothetical protein
length: 419
theoretical pI: 4.68169
theoretical MW: 46813.1
GRAVY: 0.0995227

⊟Function[edit | edit source]

TIGRFAM:
TheSEED :
- hypothetical protein
PFAM:
no clan defined Kinocilin; Kinocilin protein (PF15033; HMM-score: 15.8)
Peptidase_U (CL0472) Abi; CAAX protease self-immunity (PF02517; HMM-score: 14)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 8
LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.015793
- TAT(Tat/SPI): 0.000177
- LIPO(Sec/SPII): 0.020214
predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

GI: 57650877 NCBI
RefSeq: YP_187135 NCBI
UniProt: Q5HDM2 UniProt

⊟Protein sequence[edit | edit source]

MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFEVTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFNTFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVGLMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLIFLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHVNDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEPQHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPVTYKEDRRSSVVIDAEKHIEKTEEQSSDKNK

⊟Experimental data[edit | edit source]

experimentally validated: PeptideAtlas
protein localization: Integral membrane ^[1] ^[2] ^[3]
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e)
↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p)
↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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