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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2328 [new locus tag: SACOL_RS12240 ]
- pan locus tag?: SAUPAN005823000
- symbol: SACOL2328
- pan gene symbol?: lyrA
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2328 [new locus tag: SACOL_RS12240 ]
- symbol: SACOL2328
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 2390766..2392025
- length: 1260
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238443 NCBI
- RefSeq: YP_187135 NCBI
- BioCyc: see SACOL_RS12240
- MicrobesOnline: 913810 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTC
ATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACAC
TTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTT
TTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATT
GAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCTAATTATTGGTATGTACAGC
TTTAATACATTTGCAGATAGCTTTATTTTATTACAATCAACAGGCTTATCAGTACCTATT
ACACACATTCTGATTGGACATATTCTGATGGCGTTCGTAGTAGAATTCGGATTCCGTTCA
TACTTACAAAATATTGTTGAAACGAAAATGAACACGTTCTTTGCAAGTATTGTTGTTGGT
TTGATGTATTCAGTTTTCTCAGCAAATACAACTTATGGTACAGAATTTGCTGCTTATAAC
TTCCTTTATACATTCTCATTCTCTATGATTCTTGGTGAATTAATTAGAGCGACTAAAGGA
CGTACAATTTATATTGCAACGACATTCCATGCTTCAATGACATTCGGACTTATTTTCTTG
TTTAGCGAAGAAATCGGCGATCTATTTTCAATCAAAGTCATCGCCATTTCAACAGCAATC
GTTGCAGTAGGATACATTGGTTTAAGCTTAATTATCCGAGGTATTGCATATTTAACAACA
AGACGAAACCTTGAAGAACTTGAGCCTAATAATTATTTAGACCATGTCAATGACGATGAA
GAAACTAATCATACTGAGGCTGAAAAATCTTCTTCAAATATTAAAGATGCTGAAAAAACA
GGTGTAGCTACTGCATCAACGGTTGGTGTTGCTAAAAATGATACTGAAAATACAGTGGCT
GACGAACCAAGCATTCATGAAGGTACTGAAAAAACAGAACCTCAACATCACATAGGTAAT
CAAACTGAATCTAATCATGATGAAGATCATGACATCACTTCGGAGTCAGTAGAATCAGCA
GAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAA
ATAGAGCAATCATTAAAAGAACCTGTGACTTATAAAGAAGACAGACGTTCATCAGTTGTA
ATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2328 [new locus tag: SACOL_RS12240 ]
- symbol: SACOL2328
- description: hypothetical protein
- length: 419
- theoretical pI: 4.68169
- theoretical MW: 46813.1
- GRAVY: 0.0995227
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 8
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.015793
- TAT(Tat/SPI): 0.000177
- LIPO(Sec/SPII): 0.020214
- predicted transmembrane helices (TMHMM): 8
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFEVTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFNTFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVGLMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLIFLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHVNDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEPQHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPVTYKEDRRSSVVIDAEKHIEKTEEQSSDKNK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Integral membrane [1] [2] [3]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)