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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2425 [new locus tag: SACOL_RS12725 ]
  • pan locus tag?: SAUPAN005964000
  • symbol: SACOL2425
  • pan gene symbol?: bioF
  • synonym:
  • product: 8-amino-7-oxononanoate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2425 [new locus tag: SACOL_RS12725 ]
  • symbol: SACOL2425
  • product: 8-amino-7-oxononanoate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2483220..2484335
  • length: 1116
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAGTTTCAAGAGCAAATTGATTTATTACAACAGAAAGGGTTATATCGGTCGCTTAAA
    TCGGTAGGGTATATAGATAGACAGTATATTGAAGTAGAAAATAAGAGATGTACGAACTAT
    ACATCTAATGATTATTTAGGATTAGGTCAAATAGCGTTTGATAAGGATGATTTCGAAAGA
    TTTATGCGGAAGTATAGTTATCACTTATCAAGTTCAAGATTAATTAGTGGAAGTTCGACA
    GCTTATGAAGAAATTGAAACAATGTTAGCAGGTTGGCTCGGATATAGTGCATGTACTATC
    TTAAATAGTGGTTATGATGCTAATTTGGCGTTATTTAATATTTTCAAAAATACAAATTGT
    GTCGTGTTTTCAGATCAAGAAAATCATGCGAGTATTATTGACGGTATTAAGTTAAGTGGT
    TTAGAAAAAGTGATATATAAGCATTTAGATATTGCTGATTTAGAAAAAAGGTTAGAGAAA
    TACCCTAATCAAAATATACCAAAAATAATCATATCTGATAGTGTATTTTCAACGAATGGC
    GACGTTGTGGATATTGGTCAATTAGTCTCATTAAAGCATAAATATAACGCAACACTGATA
    CTCGATGTTTCACATAGTTTTGGAATAGAGAATTACTCGAATTATCAAGGTGTAGATATA
    CTCACTTCTAGTTTATCTAAAGCATGTGGTGCATACGGGGGTGTGATTTTAAGTTCAAAT
    GATGTGAAGGATATGTTAATTAATCACGGTAGACCACTCATCTACTCAAGTAGTTTGCCA
    ATTTATAATTTGTATTTTATAAAAAGAAACATTGAAAAGTTAATAAATGCTGATGATAGA
    CGCACTAAATTAAATAGTTTGAGTAAATATTTTAACCAAAAGTTAAAAGCACTCAATGTT
    AATTATAATAGTTCAAACTCGCCGATTAAGTTTATTGAGTTTGATGACATAGAAGCGGCT
    GAGAACATTCATCAAACATTATTAAAGCATCATGTGTTTACAAGTTATTTAAGGTATCCA
    ACTGTGACTAAGCCAATGTTAAGAATATCATTGTCGTATTTTCATACTGAACAAGATATA
    GATAGGTTGTTTGAAATTTTGCATCAAGAAGATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1116

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2425 [new locus tag: SACOL_RS12725 ]
  • symbol: SACOL2425
  • description: 8-amino-7-oxononanoate synthase
  • length: 371
  • theoretical pI: 6.74051
  • theoretical MW: 42560.9
  • GRAVY: -0.314555

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin 8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 271.5)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 219.5)
    and 9 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 184.5)
    Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 182.6)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 25)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (TIGR03588; EC 2.6.1.92; HMM-score: 23.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 22.1)
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 14.6)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 14.6)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 14.2)
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 11.8)
  • TheSEED  :
    • 8-amino-7-oxononanoate synthase (EC 2.3.1.47)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  8-amino-7-oxononanoate synthase (EC 2.3.1.47)
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 138.6)
    and 7 more
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 35.3)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 24.9)
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 20.1)
    PDDEXK (CL0236) Cas_DxTHG; CRISPR-associated (Cas) DxTHG family (PF09455; HMM-score: 17.7)
    Peptidase_CA (CL0125) Peptidase_C10; Peptidase C10 family (PF01640; HMM-score: 14.9)
    PLP_aminotran (CL0061) DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 14.1)
    TIM_barrel (CL0036) DAHP_synth_1; DAHP synthetase I family (PF00793; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007489
    • TAT(Tat/SPI): 0.000663
    • LIPO(Sec/SPII): 0.001533
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSNDYLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILNSGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEKYPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGVDILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLINADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFTSYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA (repression) regulon
    BirA(TF)important in Biotin biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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