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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2426 [new locus tag: SACOL_RS12730 ]
  • pan locus tag?: SAUPAN005965000
  • symbol: bioB
  • pan gene symbol?: bioB
  • synonym:
  • product: biotin synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2426 [new locus tag: SACOL_RS12730 ]
  • symbol: bioB
  • product: biotin synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2484313..2485320
  • length: 1008
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATTTGGCTAAACGCATATTACAAGGGGAACAATTAACAAAAGAGACTGTATTGAAA
    ATTTATGAGGATACTAATATTGATACCTTAGATTTATTAAATGAGGCGTACATTTTAAGA
    AAACATTATTTTGGTAAAAAAGTAAAATTAAACATGATTTTAAATGCTAAAAGTGGTATA
    TGTCCTGAAAATTGTGGGTACTGTGGACAATCACGAGATATTAAACAAAAACAGCGATAT
    GCTTTAATTCCAGAGGAACAAATTATCGATGGAGCAAAGGTGGCACATGATAATCATATT
    GGAACATATTGTATTGTTATGAGTGGTAGAGGACCGAGCGATAAAGAAGTTGATCATATT
    AGTAATACTGTAAGAACGATTAAATCTCAACACCCGCAACTAAAAATCTGTGCATGTTTA
    GGATTAACGAATGACGAACAAGCTAAGAAACTTAAGTCAGCTGGTGTAGACAGATATAAC
    CACAATATTAATACAAGTGAAAATTACCATGATAACGTCGTGACAACGCATAGTTATAAA
    GATAGAACAGATACGATAGAACTAATGAAAGCGAATAATATATCACCATGTTCTGGCGTG
    ATTTGTGGTATGGGAGAATCTAATCAAGATATTGTTGATATGGCATTTGCTTTAAAAGAA
    ATGGATGCCGACAGTATTCCGATTAATTTTTTGCATCCAATCAAAGGCACAAAGTTTGGA
    AGCATGGATGATTTAACACCAATGAAATGTTTAAGAATCGTAGCATTATTCCGATTAATC
    AATCCTACGAAAGAAATTCGTATTGCTGGAGGAAGAGAGGTCAACTTACGTTCGTTACAG
    CCATTAGCATTAAAAGCGGCGAATTCAATATTTGTCGGTGATTATCTAATTACTGGTGGG
    CAACCGAACCAATTAGACTACGATATGATTAATGATTTAGGCTTTGAAATTGATTATGAC
    ACTTGTGAAAATAAGGAAAACAAGAATGAAGTTTCAAGAGCAAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1008

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2426 [new locus tag: SACOL_RS12730 ]
  • symbol: BioB
  • description: biotin synthase
  • length: 335
  • theoretical pI: 6.7726
  • theoretical MW: 37558.7
  • GRAVY: -0.444179

⊟Function[edit | edit source]

  • reaction:
    EC 2.8.1.6?  ExPASy
    Biotin synthase Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin biotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 377.8)
    and 17 more
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster radical SAM maturase HydE (TIGR03956; HMM-score: 85.4)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis radical SAM protein (TIGR03910; HMM-score: 80.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis protein ThiH (TIGR02351; HMM-score: 55.1)
    Genetic information processing Protein fate Protein modification and repair 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
    Metabolism Energy metabolism Methanogenesis 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Lipoate lipoyl synthase (TIGR00510; EC 2.8.1.8; HMM-score: 44.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone putative menaquinone biosynthesis radical SAM enzyme, SCO4494 family (TIGR03700; HMM-score: 41.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone dehypoxanthine futalosine cyclase (TIGR03699; HMM-score: 35.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit (TIGR03551; EC 2.5.1.77; HMM-score: 31)
    Hypothetical proteins Conserved radical SAM domain protein, CofH subfamily (TIGR00423; HMM-score: 28.7)
    Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 23.3)
    radical SAM/SPASM domain protein, ACGX system (TIGR04340; HMM-score: 19.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin probable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 18.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofG subunit (TIGR03550; EC 2.5.1.77; HMM-score: 18.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 15.9)
    magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (TIGR02026; HMM-score: 13.1)
    His-Xaa-Ser system radical SAM maturase HxsB (TIGR03978; HMM-score: 12.5)
  • TheSEED  :
    • Biotin synthase (EC 2.8.1.6)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  Biotin synthase (EC 2.8.1.6)
  • PFAM:
    no clan defined BATS; Biotin and Thiamin Synthesis associated domain (PF06968; HMM-score: 57.3)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 57.1)
    and 1 more
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: [2Fe-2S] cluster, [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025518
    • TAT(Tat/SPI): 0.002236
    • LIPO(Sec/SPII): 0.001056
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYNHNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRAN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA (repression) regulon
    BirA(TF)important in Biotin biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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