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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02714
  • pan locus tag?: SAUPAN005965000
  • symbol: SAOUHSC_02714
  • pan gene symbol?: bioB
  • synonym:
  • product: biotin synthase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02714
  • symbol: SAOUHSC_02714
  • product: biotin synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2494978..2495985
  • length: 1008
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATTTGGCTAAACGCATATTACAAGGGGAACAATTAACAAAAGAGACTGTATTGAAA
    ATTTATGAGGATACTAATATTGATACCTTAGATTTATTAAATGAGGCGTACATTTTAAGA
    AAACATTATTTTGGTAAAAAAGTAAAATTAAACATGATTTTAAATGCTAAAAGTGGTATA
    TGTCCTGAAAATTGTGGGTACTGTGGACAATCACGAGATATTAAACAAAAACAGCGATAT
    GCTTTAATTCCAGAGGAACAAATTATCGATGGAGCAAAGGTGGCACATGATAATCATATT
    GGAACATATTGTATTGTTATGAGTGGTAGAGGACCGAGCGATAAAGAAGTTGATCATATT
    AGTAATACTGTAAGAACGATTAAATCTCAACACCCGCAACTAAAAATCTGTGCATGTTTA
    GGATTAACGAATGACGAACAAGCTAAGAAACTTAAGTCAGCTGGTGTAGACAGATATAAC
    CACAATATTAATACAAGTGAAAATTACCATGATAACGTCGTGACAACGCATAGTTATAAA
    GATAGAACAGATACGATAGAACTAATGAAAGCGAATAATATATCACCATGTTCTGGCGTG
    ATTTGTGGTATGGGAGAATCTAATCAAGATATTGTTGATATGGCATTTGCTTTAAAAGAA
    ATGGATGCCGACAGTATTCCGATTAATTTTTTGCATCCAATCAAAGGCACAAAGTTTGGA
    AGCATGGATGATTTAACACCAATGAAATGTTTAAGAATCGTAGCATTATTCCGATTAATC
    AATCCTACGAAAGAAATTCGTATTGCTGGAGGAAGAGAGGTCAACTTACGTTCGTTACAG
    CCATTAGCATTAAAAGCGGCGAATTCAATATTTGTCGGTGATTATCTAATTACTGGTGGG
    CAACCGAACCAATTAGACTACGATATGATTAATGATTTAGGCTTTGAAATTGATTATGAC
    ACTTGTGAAAATAAGGAAAACAAGAATGAAGTTTCAAGAGCAAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1008

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_02714
  • symbol: SAOUHSC_02714
  • description: biotin synthase
  • length: 335
  • theoretical pI: 6.7726
  • theoretical MW: 37558.7
  • GRAVY: -0.444179

Function[edit | edit source]

  • reaction:
    EC 2.8.1.6?  ExPASy
    Biotin synthase Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin biotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 377.8)
    and 17 more
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster radical SAM maturase HydE (TIGR03956; HMM-score: 85.4)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis radical SAM protein (TIGR03910; HMM-score: 80.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis protein ThiH (TIGR02351; HMM-score: 55.1)
    Genetic information processing Protein fate Protein modification and repair 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
    Metabolism Energy metabolism Methanogenesis 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Lipoate lipoyl synthase (TIGR00510; EC 2.8.1.8; HMM-score: 44.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone putative menaquinone biosynthesis radical SAM enzyme, SCO4494 family (TIGR03700; HMM-score: 41.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone dehypoxanthine futalosine cyclase (TIGR03699; HMM-score: 35.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit (TIGR03551; EC 2.5.1.77; HMM-score: 31)
    Hypothetical proteins Conserved radical SAM domain protein, CofH subfamily (TIGR00423; HMM-score: 28.7)
    Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 23.3)
    radical SAM/SPASM domain protein, ACGX system (TIGR04340; HMM-score: 19.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin probable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 18.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofG subunit (TIGR03550; EC 2.5.1.77; HMM-score: 18.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 15.9)
    magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (TIGR02026; HMM-score: 13.1)
    His-Xaa-Ser system radical SAM maturase HxsB (TIGR03978; HMM-score: 12.5)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  Biotin synthase (EC 2.8.1.6)
    and 1 more
    Biotin synthesis & utilization  Biotin synthase (EC 2.8.1.6)
  • PFAM:
    no clan defined BATS; Biotin and Thiamin Synthesis associated domain (PF06968; HMM-score: 57.3)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 57.1)
    and 1 more
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 13.9)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: [2Fe-2S] cluster, [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025518
    • TAT(Tat/SPI): 0.002236
    • LIPO(Sec/SPII): 0.001056
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYNHNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRAN

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]