From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2509 [new locus tag: SACOL_RS13150 ]
  • pan locus tag?: SAUPAN006097000
  • symbol: fnbB
  • pan gene symbol?: fnbB
  • synonym:
  • product: fibronectin binding protein B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2509 [new locus tag: SACOL_RS13150 ]
  • symbol: fnbB
  • product: fibronectin binding protein B
  • replicon: chromosome
  • strand: -
  • coordinates: 2565936..2568758
  • length: 2823
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    GTGAAAAGCAATCTTAGATACGGCATAAGAAAACACAAATTGGGAGCGGCCTCAGTATTC
    TTAGGAACAATGATCGTTGTTGGAATGGGACAAGAAAAAGAAGCTGCAGCATCGGAACAA
    AACAATACTACAGTAGAGGAAAGTGGGAGTTCAGCTACTGAAAGTAAAGCAAGCGAAACA
    CAAACAACTACAAATAACGTTAATACAATAGATGAAACACAATCATACAGCGCGACATCA
    ACTGAGCAACCATCACAATCAACACAAGTAACAACAGAAGAAGCACCGAAAACTGTGCAA
    GCACCAAAAGTAGAAACTTCGCGAGTTGATTTGCCATCGGAAAAAGTTGCTGATAAGGAA
    ACTACAGGAACTCAAGTTGACATAGCTCAACCAAGTAACGTCTCAGAAATTAAACCAAGA
    ATGAAAAGATCAACTGACGTTACAGCAGTTGCAGAGAAAGAAGTAGTGGAAGAAACTAAA
    GCGACAGGTACAGATGTAACAAATAAAGTGGAAGTAGAAGAAGGTAGTGAAATTGTAGGA
    CATAAACAAGATACGAATGTTGTAAATCCTCATAACGCAGAAAGAGTAACCTTGAAATAT
    AAATGGAAATTTGGAGAAGGAATTAAGGCGGGAGATTATTTTGATTTCACATTAAGCGAT
    AATGTTGAAACTCATGGTATCTCAACACTGCGTAAAGTTCCGGAGATAAAAAGTACAGAT
    GGTCAAGTTATGGCGACAGGAGAAATAATTGGAGAAAGAAAAGTTAGATATACGTTTAAA
    GAATATGTACAAGAAAAGAAAGATTTAACTGCTGAATTATCTTTAAATCTATTTATTGAT
    CCTACAACAGTGACGCAAAAAGGTAACCAAAATGTTGAAGTTAAATTGGGTGAGACTACG
    GTTAGCAAAATATTTAATATTCAATATTTAGGTGGAGTTAGAGATAATTGGGGAGTAACA
    GCTAATGGTCGAATTGATACTTTAAATAAAGTAGATGGGAAATTTAGTCATTTTGCGTAC
    ATGAAACCTAACAACCAGTCGTTAAGCTCTGTGACAGTAACTGGTCAAGTAACTAAAGGA
    AATAAACCAGGGGTTAATAATCCAACAGTTAAGGTATATAAACACATTGGTTCAGACGAT
    TTAGCTGAAAGCGTATATGCAAAGCTTGATGATGTCAGCAAATTTGAAGATGTGACTGAT
    AATATGAGTTTAGATTTTGATACTAATGGTGGTTATTCTTTAAACTTTAATAATTTAGAC
    CAAAGTAAAAATTATGTAATAAAATATGAAGGGTATTATGATTCAAATGCTAGCAACTTA
    GAATTTCAAACACACCTTTTTGGATATTATAACTATTATTATACAAGTAATTTAACTTGG
    AAAAATGGCGTTGCATTTTACTCTAATAACGCTCAAGGCGACGGCAAAGATAAACTAAAG
    GAACCTATTATAGAACATAGTACTCCTATCGAACTTGAATTTAAATCAGAGCCGCCAGTG
    GAGAAGCATGAATTGACTGGTACAATCGAAGAAAGTAATGATTCTAAGCCAATTGATTTT
    GAATATCATACAGCTGTTGAAGGTGCAGAAGGTCATGCAGAAGGTACCATTGAAACTGAA
    GAAGATTCTATTCATGTAGACTTTGAAGAATCGACACATGAAAATTCAAAACATCATGCT
    GATGTTGTTGAATATGAAGAAGATACAAACCCAGGTGGTGGTCAGGTTACTACTGAGTCT
    AACCTAGTTGAATTTGACGAAGATTCTACAAAAGGTATTGTAACTGGTGCTGTTAGCGAT
    CATACAACAATTGAAGATACGAAAGAATATACGACTGAAAGTAATCTGATTGAACTAGTA
    GATGAACTACCTGAAGAACATGGTCAAGCGCAAGGACCAATCGAGGAAATTACTGAAAAC
    AATCATCATATTTCTCATTCTGGTTTAGGAACTGAAAATGGTCACGGTAATTATGGCGTG
    ATTGAAGAAATCGAAGAAAATAGCCACGTGGATATTAAGAGTGAATTAGGTTACGAAGGT
    GGCCAAAATAGCGGTAATCAGTCATTTGAGGAAGACACAGAAGAAGATAAACCGAAATAT
    GAACAAGGTGGCAATATCGTAGATATCGATTTCGATAGTGTACCTCAAATTCATGGTCAA
    AATAATGGTAACCAATCATTCGAAGAAGATACAGAGAAAGACAAACCTAAGTATGAACAA
    GGTGGTAATATCATTGATATCGACTTCGACAGTGTGCCACATATTCACGGATTCAATAAG
    CACACTGAAATTATTGAAGAAGATACAAATAAAGATAAACCAAATTATCAATTCGGTGGA
    CACAATAGTGTTGACTTTGAAGAAGATACACTTCCACAAGTAAGTGGTCATAATGAAGGT
    CAACAAACGATTGAAGAAGATACAACACCTCCAATCGTGCCACCAACGCCACCGACACCA
    GAAGTACCAAGCGAGCCGGAAACACCAACACCACCGACACCAGAAGTACCAAGCGAGCCG
    GAAACACCAACACCGCCAACGCCAGAGGTACCAACTGAACCTGGTAAACCAATACCACCT
    GCTAAAGAAGAACCTAAAAAACCTTCTAAACCAGTGGAACAAGGTAAAGTAGTAACACCT
    GTTATTGAAATCAATGAAAAGGTTAAAGCAGTGGTACCAACTAAAAAAGCACAATCTAAG
    AAATCTGAACTACCTGAAACAGGTGGAGAAGAATCAACAAACAACGGCATGTTGTTCGGC
    GGATTATTTAGCATTTTAGGTTTAGCGTTATTACGCAGAAATAAAAAGAATCACAAAGCA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2823

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2509 [new locus tag: SACOL_RS13150 ]
  • symbol: FnbB
  • description: fibronectin binding protein B
  • length: 940
  • theoretical pI: 4.36624
  • theoretical MW: 103554
  • GRAVY: -0.87117

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 26.7)
    and 1 more
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 20.8)
  • TheSEED  :
    • Fibronectin binding protein FnbB
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Fibronectin binding protein FnbB
  • PFAM:
    Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 133.5)
    no clan defined Fn_bind; Fibronectin binding repeat (PF02986; HMM-score: 107.5)
    and 2 more
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 34.5)
    YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 32)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: found LPxTG motif :LPETG
  • SignalP: Signal peptide SP(Sec/SPI) length 37 aa
    • SP(Sec/SPI): 0.879583
    • TAT(Tat/SPI): 0.010762
    • LIPO(Sec/SPII): 0.095299
    • Cleavage Site: CS pos: 37-38. AAA-SE. Pr: 0.7097
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTTVEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAPKVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETKATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTLSDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLNLFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGKFSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDVSKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYYNYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGTIEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYEEDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGKPIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTNNGMLFGGLFSILGLALLRRNKKNHKA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Sortase substrate [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

T J Foster, M Höök
Surface protein adhesins of Staphylococcus aureus.
Trends Microbiol: 1998, 6(12);484-8
[PubMed:10036727] [WorldCat.org] [DOI] (P p)

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2509&oldid=92661"