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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2518 [new locus tag: SACOL_RS13185 ]
  • pan locus tag?: SAUPAN006134000
  • symbol: SACOL2518
  • pan gene symbol?: relP
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2518 [new locus tag: SACOL_RS13185 ]
  • symbol: SACOL2518
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2577779..2578471
  • length: 693
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTATGTAGATCGAAAACCATCACTATATTTAGAGGATTTGCGACATGATTTTAAAAAT
    AGTTTAAGTAAATTTGAAAATGGTGATGAAGCATTTGATACGTTATTAGGTTTCGTAGAG
    TTAGATCATATTTATTCGTCAGCACTAAAGGAAATAAGCACTAAACTGAGTATTTTAGAT
    GACAATTTCAATCACATTTATAAACACAATCCTATACATCATATGGAGCGACGTGTGAAA
    GAAATGCGTAGTTTAATAGAAAAGCTTAATCGTAAAGGATTACAGATTAGCGCAGAAACT
    GCCAAAGAACATATACTGGATATTGCCGGAATTCGCGTAGTATGTAATTACTTAGATGAT
    ATTTATTTGATTGAAGAGATGTTGCTTAAACAAGAAGACGTACAATTGATAAAACGTAAA
    GATTATATTCAGCACCCTAAAGAAAATGGTTACCGCAGTTTACATATCGTTGTATCTATT
    CCAGTCTTTTTAGCAGAACGCGTTGAGGTATTGCCTGTTGAAATTCAAATTAGAACGATA
    GGTATGGATATGTGGGCAAGTTTAGAACATAAAATACGTTATAAAAACAATGCAGAGACG
    GAAAAGTATCGAGATTTACTGAAAGAATGTGCGACAGAGATTACTGAAGTTGAAGATAAA
    TTACAACAAATTCATTCTGAAATAACAGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    693

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2518 [new locus tag: SACOL_RS13185 ]
  • symbol: SACOL2518
  • description: hypothetical protein
  • length: 230
  • theoretical pI: 5.77612
  • theoretical MW: 27165
  • GRAVY: -0.47087

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions RelA/SpoT family protein (TIGR00691; HMM-score: 59.7)
  • TheSEED  :
    • ((p)p)pGpp synthetase RelP
    CBSS-222523.1.peg.1311  GTP pyrophosphokinase (EC 2.7.6.5)
  • PFAM:
    NTP_transf (CL0260) RelA_SpoT; Region found in RelA / SpoT proteins (PF04607; HMM-score: 128.8)
    and 4 more
    Traffic (CL0147) V-SNARE; Vesicle transport v-SNARE protein N-terminus (PF05008; HMM-score: 15.5)
    no clan defined UvsW; ATP-dependant DNA helicase UvsW (PF11637; HMM-score: 14.2)
    RNase_H2-Ydr279; Ydr279p protein family (RNase H2 complex component) (PF09468; HMM-score: 14)
    DUF4140; N-terminal domain of unknown function (DUF4140) (PF13600; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00153
    • TAT(Tat/SPI): 0.000146
    • LIPO(Sec/SPII): 0.000279
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHIYSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAKEHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSIPVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVEDKLQQIHSEITE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 192 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Tobias Geiger, Benjamin Kästle, Fabio Lino Gratani, Christiane Goerke, Christiane Wolz
Two small (p)ppGpp synthases in Staphylococcus aureus mediate tolerance against cell envelope stress conditions.
J Bacteriol: 2014, 196(4);894-902
[PubMed:24336937] [WorldCat.org] [DOI] (I p)

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