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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02811
  • pan locus tag?: SAUPAN006134000
  • symbol: SAOUHSC_02811
  • pan gene symbol?: relP
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02811
  • symbol: SAOUHSC_02811
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2589722..2590414
  • length: 693
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTATGTAGATCGAAAACCATCACTATATTTAGAGGATTTGCGACATGATTTTAAAAAT
    AGTTTAAGTAAATTTGAAAATGGTGATGAAGCATTTGATACGTTATTAGGTTTCGTAGAG
    TTAGATCATATTTATTCGTCAGCACTAAAGGAAATAAGCACTAAACTGAGTATTTTAGAT
    GACAATTTCAATCACATTTATAAACACAATCCTATACATCATATGGAGCGACGTGTGAAA
    GAAATGCGTAGTTTAATAGAAAAGCTTAATCGTAAAGGATTACAGATTAGCGCAGAAACT
    GCCAAAGAACATATACTGGATATTGCCGGAATTCGCGTAGTATGTAATTACTTAGATGAT
    ATTTATTTGATTGAAGAGATGTTGCTTAAACAAGAAGACGTACAATTGATAAAACGTAAA
    GATTATATTCAGCACCCTAAAGAAAATGGTTACCGCAGTTTACATATCGTTGTATCTATT
    CCAGTCTTTTTAGCAGAACGCGTTGAGGTATTGCCTGTTGAAATTCAAATTAGAACGATA
    GGTATGGATATGTGGGCAAGTTTAGAACATAAAATACGTTATAAAAACAATGCAGAGACG
    GAAAAGTATCGAGATTTACTGAAAGAATGTGCGACAGAGATTACTGAAGTTGAAGATAAA
    TTACAACAAATTCATTCTGAAATAACAGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    693

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02811
  • symbol: SAOUHSC_02811
  • description: hypothetical protein
  • length: 230
  • theoretical pI: 5.77612
  • theoretical MW: 27165
  • GRAVY: -0.47087

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions RelA/SpoT family protein (TIGR00691; HMM-score: 59.7)
  • TheSEED  :
    • ((p)p)pGpp synthetase RelP
    CBSS-222523.1.peg.1311  GTP pyrophosphokinase (EC 2.7.6.5)
  • PFAM:
    NTP_transf (CL0260) RelA_SpoT; Region found in RelA / SpoT proteins (PF04607; HMM-score: 128.8)
    and 4 more
    Traffic (CL0147) V-SNARE; Vesicle transport v-SNARE protein N-terminus (PF05008; HMM-score: 15.5)
    no clan defined UvsW; ATP-dependant DNA helicase UvsW (PF11637; HMM-score: 14.2)
    RNase_H2-Ydr279; Ydr279p protein family (RNase H2 complex component) (PF09468; HMM-score: 14)
    DUF4140; N-terminal domain of unknown function (DUF4140) (PF13600; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00153
    • TAT(Tat/SPI): 0.000146
    • LIPO(Sec/SPII): 0.000279
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHIYSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAKEHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSIPVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVEDKLQQIHSEITE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Tobias Geiger, Benjamin Kästle, Fabio Lino Gratani, Christiane Goerke, Christiane Wolz
Two small (p)ppGpp synthases in Staphylococcus aureus mediate tolerance against cell envelope stress conditions.
J Bacteriol: 2014, 196(4);894-902
[PubMed:24336937] [WorldCat.org] [DOI] (I p)

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