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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2527 [new locus tag: SACOL_RS13235 ]
  • pan locus tag?: SAUPAN006159000
  • symbol: SACOL2527
  • pan gene symbol?: fbp
  • synonym:
  • product: fructose-1,6-bisphosphatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2527 [new locus tag: SACOL_RS13235 ]
  • symbol: SACOL2527
  • product: fructose-1,6-bisphosphatase
  • replicon: chromosome
  • strand: +
  • coordinates: 2587978..2589942
  • length: 1965
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACTCAAATTACTGAAAAAGAATTAAAAAAGAAGTATTTAGATTTACTATCCCAAAAT
    TTTGATACTCCAGAAAAACTTGCAACTGAAATTATCAATTTAGAATCAATTTTAGAATTA
    CCTAAAGGTACGGAACATTTCGTCAGCGATTTACATGGTGAATATGAAGCTTTCCAACAC
    GTATTACGCAACGGTTCTGGGAACGTGCGAGCGAAAATCAATGATATTTTCAAAGAGAGA
    CTTTCAACTAAGGAGCTTAATGACTTAACTGCTCTTGTCTACTATCCAGAAGACAAATTA
    AAATTGATTAAAAGTGATTTCCAAAATTGCGGTCAACTTAATGTCTGGTATATCACAACA
    ATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTG
    CGTAAAGCATTGCCAAAACAATACGTTTATATTATTGAGGAACTACTGTATAAAAGTAAT
    GAATATCAAAATAAAAAATCATATTACGAAACACTTGTTAACCAAGTAATTGAACTTAAA
    CAGGCAGATGATTTAATTATTGGACTCGCTTATTCCGTACAACGCTTAGTCGTCGATCAT
    TTACACGTTGTCGGTGATATTTATGATCGTGGACCACAACCAGATAAAATTATGGATACA
    CTGATTAATTATCATTCCCTAGATATTCAATGGGGTAATCATGATGTGCTTTGGGTTGGA
    GCCTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGAT
    AATTTAGATATTATCGAAGACGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCT
    GAAAAATACTATGACGCAGATAATCCTGCTTTTAAGCCTAAAAAAAGACCTGACAAACAC
    GAACGTTTAACTCAACGTGAAGAAAGTCAAATTACTAAAATTCATCAAGCTATTGCGATG
    ATTCAATTCAAGTTAGAAATACCAATTATTAAACGTCGTCCAAATTTCGAAATGGAAGAA
    CGTCTTGTGCTTGAAAAGGTTAATTATGATACAAATGAAATTACAGTTTATGGTAATACA
    TACCCATTGAAAGACACATGTTTCCAAACTGTCAATCGTGATAATCCAGCAGAATTACTA
    CCTGAAGAAGAAGAAGTCATGAATAAACTATTATTGTCATTCCAACAATCTGAAAAATTA
    CGTCGTCATATGTCTTTCTTGATGCGTAAAGGCTCTCTTTACTTACCATATAATGGCAAT
    TTACTCATTCATGGTTGTATTCCAGTTGATGAAAATGGTGAGATGGAATCATTTGAAATT
    GATGGTCATACTTACAGCGGCCAAGAATTATTAGATGTGTTTGAGTATCATGTCCGTAAA
    TCATTTGATGAAAAAGAAAATACTGATGACTTATCGACGGATTTAGTTTGGTATTTATGG
    ACTGGGAAATATTCGTCACTATTTGGTAAACGTGCCATGACTACGTTTGAGCGATACTTT
    ATTGCAGATAAAGCTTCTCATAAAGAAGAAAAGAATCCGTACTATCATCTTCGTGAAGAT
    GTGAATATGGTTCGTAAAATGCTCAGTGATTTCGGATTAAATCCAGATGAAGGACGCATT
    ATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGA
    AAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCA
    GGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAAC
    GCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGAT
    AAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTGGTAAAGATCTTCAAGCTCAA
    ATAGATATTTTGAAAATGTTAATGCATGATCGTTACTTAGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1965

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2527 [new locus tag: SACOL_RS13235 ]
  • symbol: SACOL2527
  • description: fructose-1,6-bisphosphatase
  • length: 654
  • theoretical pI: 6.04366
  • theoretical MW: 76174.5
  • GRAVY: -0.520336

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.3.11?  ExPASy
    Fructose-bisphosphatase D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate
  • TIGRFAM:
  • TheSEED  :
    • Fructose-1,6-bisphosphatase, Bacillus type (EC 3.1.3.11)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  Fructose-1,6-bisphosphatase, Bacillus type (EC 3.1.3.11)
  • PFAM:
    Calcineurin (CL0163) FBPase_2; Firmicute fructose-1,6-bisphosphatase (PF06874; HMM-score: 940.9)
    and 1 more
    no clan defined GP24_25; Tail assembly protein Gp24 and Gp25 (PF17388; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002427
    • TAT(Tat/SPI): 0.000086
    • LIPO(Sec/SPII): 0.000178
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGTEHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKLIKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSNEYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIMDTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPLLTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRPNFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLLSFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQELLDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHKEEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVIDGGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQRKKIRDTNIGKDLQAQIDILKMLMHDRYLD

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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