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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2554.1 [new locus tag: SACOL_RS13375 ]
  • pan locus tag?: SAUPAN006198000
  • symbol: SACOL2554.1
  • pan gene symbol?: cidA
  • synonym:
  • product: LrgA family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2554.1 [new locus tag: SACOL_RS13375 ]
  • symbol: SACOL2554.1
  • product: LrgA family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2613806..2614201
  • length: 396
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGCACAAAGTCCAATTAATAATCAAACTACTACTACAACTAGGAATCATCATTGTGATT
    ACTTATATTGGCACAGAAATTCAAAAGATTTTTCATCTTCCCTTAGCCGGCAGTATTGTT
    GGTCTATTTTTATTTTATTTACTATTACAATTTAAGATTGTACCGCTAACTTGGGTAGAA
    GACGGTGCAAACTTTTTATTAAAGACGATGGTCTTTTTCTTCATACCGTCAGTTGTAGGT
    ATTATGGATGTTGCTTCCGAAATTACGCTAAATTATATACTCTTTTTCGCAGTCATTATC
    ATAGGAACATGTATCGTTGCATTATCTTCAGGTTATATTGCTGAAAAAATGTCTGTTAAA
    CATAAACATCGTAAAGGTGTAGACGCTTATGAATGA
    60
    120
    180
    240
    300
    360
    396

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2554.1 [new locus tag: SACOL_RS13375 ]
  • symbol: SACOL2554.1
  • description: LrgA family protein
  • length: 131
  • theoretical pI: 9.01426
  • theoretical MW: 14729.8
  • GRAVY: 1.04962

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Holin-like protein CidA
    Regulation and Cell signaling Programmed Cell Death and Toxin-antitoxin Systems Murein hydrolase regulation and cell death  Holin-like protein CidA
  • PFAM:
    no clan defined LrgA; LrgA family (PF03788; HMM-score: 83.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004866
    • TAT(Tat/SPI): 0.000114
    • LIPO(Sec/SPII): 0.016401
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFLFYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIGTCIVALSSGYIAEKMSVKHKHRKGVDAYE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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