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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2619 [new locus tag: SACOL_RS13705 ]
  • pan locus tag?: SAUPAN006300000
  • symbol: SACOL2619
  • pan gene symbol?: bcaP
  • synonym:
  • product: amino acid permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2619 [new locus tag: SACOL_RS13705 ]
  • symbol: SACOL2619
  • product: amino acid permease
  • replicon: chromosome
  • strand: +
  • coordinates: 2676060..2677508
  • length: 1449
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGAAGTTTTTTCAATAAAATAGCACGAAAAGAGGATCCGGCTATCTATCAAAATAAA
    GATGGTCATTTAAAGCGTACACTTCGGGTGCGTGATTTCTTAGCTTTAGGTGTAGGAACA
    ATTGTATCGACATCTATCTTTACGCTACCTGGCATTGTTGCTGCAGAACATGCAGGACCG
    GCCGTTGCGTTATCATTCTTACTCGCTGCTATTGTTGCTGGTTTAGTTGCATTTACTTAT
    GCAGAAATGGCTGCCGCTATGCCATTTGCAGGTTCAGCCTATTCTTGGGTCAATGTATTA
    TTTGGTGAATTTTTTGGATGGGTTGCCGGTTGGGCTCTATTAGCTGAATATTTTATCGCC
    GTAGCCTTTGTTGCATCAGGATTCTCAGCGAATTTACGCGGACTTGTGAAACCAATTGGC
    ATCGAATTACCTGCAGCATTATCAAATCCATTTGGTACAAATGGCGGTTTTATCGATATT
    ATTGCTGCTATCGTTATTTTATTAACTGCATTATTACTATCACGTGGTATGTCGGAAGCA
    GCTCGTATGGAAAATATTTTAGTTATTTTAAAAGTATTAGCTATTATTTTATTTGTCATC
    GTAGGTTTAACAGCAATAAATGTTAGTAACTATGTGCCATTTATTCCAGAACACAAAGTA
    ACTGCTACAGGTGACTTTGGTGGATGGCAAGGCATATATGCTGGTGTTTCAATGATTTTC
    TTAGCGTATATCGGTTTCGATTCTATCGCAGCAAACTCAGCAGAAGCACTTGATCCTCAA
    AAGACAATGCCTAGAGGTATTCTTGGTTCTTTAAGCGTTGCTATCGTATTATTTATTGCT
    GTAGCACTTGTGTTAGTTGGTATGTTCCATTACTCACAATACGCAAACAATGCTGAACCT
    GTTGGTTGGGCTTTACGTCAAAGTGGTCATGGTGTTGTAGCAGCTATTGTTCAAGCTATC
    TCTGTTATCGGTATGTTTACAGCATTAATTGGTATGATGTTAGCAGGCTCACGTTTACTT
    TATTCATTTGGACGTGACGGCTTATTACCTTCATGGTTAAGCCACTTAAACGACAAACAT
    TTACCTAATCGCGCACTAGTTATACTTACTATTATTGGTGTTTTAATTGGTTCTATGTTC
    CCATTCGCATTTTTAGCACAATTAATTTCAGCAGGTACACTTGTTGCATTTATGTTCGTT
    TCATTAGCAATGTATCGTTTGAGAAAGCGTGAAGGTAAAGATCTACCAATTCCTGCATTT
    AAATTACCTTTATATCCTGTGTTACCAGCAATTACATTTGTTTTAGTATTGCTAGTATTC
    TGGGGATTAGGATTCGAAGCGAAATTATACACTTTAATTTGGTTTATTGTTGGTATCATC
    CTATATTTAAGTTATGGACTTAGACATTCTAAGAAAAATGACGTAGCTGAATACCACCCA
    CCAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1449

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2619 [new locus tag: SACOL_RS13705 ]
  • symbol: SACOL2619
  • description: amino acid permease
  • length: 482
  • theoretical pI: 9.73071
  • theoretical MW: 51893.3
  • GRAVY: 0.837344

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 325.6)
    and 13 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 252.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 114.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 112.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 90.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 80.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 76.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 68.4)
    permease, urea carboxylase system (TIGR03428; HMM-score: 66.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (TIGR00907; HMM-score: 50.2)
    histidine-histamine antiporter (TIGR04298; HMM-score: 42.2)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 36)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 31)
    Metabolism Transport and binding proteins Other K-Cl cotransporter (TIGR00930; HMM-score: 2.1)
  • TheSEED  :
    • Uncharacterized amino acid permease, GabP family
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 194.5)
    and 2 more
    AA_permease; Amino acid permease (PF00324; HMM-score: 129.1)
    AA_permease_C; C-terminus of AA_permease (PF13906; HMM-score: 29.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.123512
    • TAT(Tat/SPI): 0.133254
    • LIPO(Sec/SPII): 0.002933
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVSTSIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFGEFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDIIAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEHKVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIVLFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMMLAGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAGTLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLYTLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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