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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2636 [new locus tag: SACOL_RS13800 ]
  • pan locus tag?: SAUPAN006323000
  • symbol: SACOL2636
  • pan gene symbol?: citM
  • synonym:
  • product: citrate transporter, permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2636 [new locus tag: SACOL_RS13800 ]
  • symbol: SACOL2636
  • product: citrate transporter, permease
  • replicon: chromosome
  • strand: -
  • coordinates: 2696178..2697548
  • length: 1371
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATAGTGATAATATGTGGTTAACAGTAATGGGGCTCATTATTATTATTTCAATTGTA
    GGTTTACTCATTGCCAAAAAGATAAATCCAGTTGTAGGTATGACAATCATACCTTGCTTA
    GGGGCAATGATTTTAGGATATAGTGTGACAGATTTGGTTGGATTTTTTGCTAAAGGGTTA
    GATCAAGTCATCAACGTTGTTATTATGTTTATCTTTGCCATTATTTTCTTTGGCATCATG
    AACGATAGTGGTTTATTCAAGCCGCTTGTCAAACGCTTAATATTAATGACACGAGGCAAT
    GTCGTCATTGTCTGTGCAATGACAGCTTTAATTGGCACAATAGCCCAATTAGATGGGGCC
    GGTGCGGTAACATTTTTGCTTTCTATTCCTGCATTATTACCTTTATATAAAGCGTTAAAT
    ATGAATAAATATTTATTGATTTTACTATTAGCATTAAGCGCGGCGATTATGAACATGGTA
    CCTTGGGGAGGTCCAATGGCTCGTGTAGCTGCAGTGTTAAAAGCCAAAAGTGTCAATGAA
    TTATGGTATGGATTAATACCTATTCAAATAATAGGTTTCATTCTTGTTATGTTGTTTGCG
    GTATATCTTGGATTTAAAGAACAGAAACGTATCAAAAAAGCAATAGAGAGAAATGAATTA
    CCGCAAACACAAGATATAGATGTACATAAATTAGTTGAAGTATATGAACGAGATCAAGAT
    GTAAGGTTTCCTGTAAAAGGACGTGCAAGAACAAAATCATGGATAAAATGGGTGAATACA
    GCTTTAACTTTAGCTGTTATTCTATCGATGTTAATAAATATTGCGCCACCTGAATTTGCA
    TTCATGATAGGTGTTTCGTTGGCACTTGTTATTAATTTTAAATCAGTGGATGAACAAATG
    GAACGATTAAGAGCGCATGCGCCGAATGCATTAATGATGGCTGCAGTGATTATTGCAGCA
    GGTATGTTTTTAGGTGTACTAAATGAAACCGGTATGCTTAAAGCGATTGCGACCAATTTA
    ATCAAAGTGATTCCTGCAGAAGTAGGACCATACTTGCATATTATTGTAGGTTTACTTGGC
    GTACCATTAGATTTACTAACTAGCACAGACGCTTATTATTTTGCTGTGTTACCGATTGTC
    GAACAAACAGCAGGGCAATTTGGTGTACCGTCTGTATCAACAGCTTATTCAATGGTCATA
    GGGAATATTATAGGTACATTTGTCAGCCCATTTTCACCAGCCTTATGGTTGGCAATTGGT
    TTAGCAGAGGCAAACATGGGCACGTATATTAAGTATGCATTCTTTTGGATTTGGGGATTC
    GCTATCGTTATGTTAGTAATTGCAATGTTGATGGGCATTGTGACGATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1371

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2636 [new locus tag: SACOL_RS13800 ]
  • symbol: SACOL2636
  • description: citrate transporter, permease
  • length: 456
  • theoretical pI: 9.61164
  • theoretical MW: 49771.1
  • GRAVY: 0.952412

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids citrate transporter (TIGR00784; HMM-score: 279.8)
    and 1 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 13.3)
  • TheSEED  :
    • Citrate/H+ symporter of CitMHS family
  • PFAM:
    IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 131.5)
    and 7 more
    DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 26.9)
    DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 23.4)
    Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 21.6)
    Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 18.3)
    no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 11.9)
    Caudo_bapla_RBP; Receptor-binding protein of phage tail base-plate Siphoviridae, head (PF08931; HMM-score: 11)
    LapA_dom; Lipopolysaccharide assembly protein A domain (PF06305; HMM-score: 9.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.03563
    • TAT(Tat/SPI): 0.000627
    • LIPO(Sec/SPII): 0.011641
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMILGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVVIVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMVPWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERNELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAPPEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLKAIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSVSTAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAMLMGIVTI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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