PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS13800 [old locus tag: SACOL2636 ]
• pan locus tag^?: SAUPAN006323000
• symbol: SACOL_RS13800
• pan gene symbol^?: citM
• synonym:
• product: citrate transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS13800 [old locus tag: SACOL2636 ]
• symbol: SACOL_RS13800
• product: citrate transporter
• replicon: chromosome
• strand: -
• coordinates: 2696178..2697548
• length: 1371
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2696178..2697548) NCBI
• BioCyc: SACOL_RS13800 BioCyc
• MicrobesOnline: see SACOL2636

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1321
  
  ATGAATAGTGATAATATGTGGTTAACAGTAATGGGGCTCATTATTATTATTTCAATTGTA
  GGTTTACTCATTGCCAAAAAGATAAATCCAGTTGTAGGTATGACAATCATACCTTGCTTA
  GGGGCAATGATTTTAGGATATAGTGTGACAGATTTGGTTGGATTTTTTGCTAAAGGGTTA
  GATCAAGTCATCAACGTTGTTATTATGTTTATCTTTGCCATTATTTTCTTTGGCATCATG
  AACGATAGTGGTTTATTCAAGCCGCTTGTCAAACGCTTAATATTAATGACACGAGGCAAT
  GTCGTCATTGTCTGTGCAATGACAGCTTTAATTGGCACAATAGCCCAATTAGATGGGGCC
  GGTGCGGTAACATTTTTGCTTTCTATTCCTGCATTATTACCTTTATATAAAGCGTTAAAT
  ATGAATAAATATTTATTGATTTTACTATTAGCATTAAGCGCGGCGATTATGAACATGGTA
  CCTTGGGGAGGTCCAATGGCTCGTGTAGCTGCAGTGTTAAAAGCCAAAAGTGTCAATGAA
  TTATGGTATGGATTAATACCTATTCAAATAATAGGTTTCATTCTTGTTATGTTGTTTGCG
  GTATATCTTGGATTTAAAGAACAGAAACGTATCAAAAAAGCAATAGAGAGAAATGAATTA
  CCGCAAACACAAGATATAGATGTACATAAATTAGTTGAAGTATATGAACGAGATCAAGAT
  GTAAGGTTTCCTGTAAAAGGACGTGCAAGAACAAAATCATGGATAAAATGGGTGAATACA
  GCTTTAACTTTAGCTGTTATTCTATCGATGTTAATAAATATTGCGCCACCTGAATTTGCA
  TTCATGATAGGTGTTTCGTTGGCACTTGTTATTAATTTTAAATCAGTGGATGAACAAATG
  GAACGATTAAGAGCGCATGCGCCGAATGCATTAATGATGGCTGCAGTGATTATTGCAGCA
  GGTATGTTTTTAGGTGTACTAAATGAAACCGGTATGCTTAAAGCGATTGCGACCAATTTA
  ATCAAAGTGATTCCTGCAGAAGTAGGACCATACTTGCATATTATTGTAGGTTTACTTGGC
  GTACCATTAGATTTACTAACTAGCACAGACGCTTATTATTTTGCTGTGTTACCGATTGTC
  GAACAAACAGCAGGGCAATTTGGTGTACCGTCTGTATCAACAGCTTATTCAATGGTCATA
  GGGAATATTATAGGTACATTTGTCAGCCCATTTTCACCAGCCTTATGGTTGGCAATTGGT
  TTAGCAGAGGCAAACATGGGCACGTATATTAAGTATGCATTCTTTTGGATTTGGGGATTC
  GCTATCGTTATGTTAGTAATTGCAATGTTGATGGGCATTGTGACGATTTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS13800 [old locus tag: SACOL2636 ]
• symbol: SACOL_RS13800
• description: citrate transporter
• length: 456
• theoretical pI: 9.61164
• theoretical MW: 49771.1
• GRAVY: 0.952412

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids citrate transporter (TIGR00784; HMM-score: 279.8)
  and 1 more
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 13.3)
• TheSEED: see SACOL2636
• PFAM:
  IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 131.5)
  and 7 more
  DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 26.9)
  DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 23.4)
  Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 21.6)
  Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 18.3)
  no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 11.9)
  Caudo_bapla_RBP; Receptor-binding protein of phage tail base-plate Siphoviridae, head (PF08931; HMM-score: 11)
  LapA_dom; Lipopolysaccharide assembly protein A domain (PF06305; HMM-score: 9.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.03563
  • TAT(Tat/SPI): 0.000627
  • LIPO(Sec/SPII): 0.011641
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 447006243 NCBI
• RefSeq: WP_001083499 NCBI
• UniProt: see SACOL2636

⊟Protein sequence

• MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMILGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVVIVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMVPWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERNELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAPPEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLKAIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSVSTAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAMLMGIVTI

⊟Experimental data

• experimentally validated: see SACOL2636
• protein localization: see SACOL2636
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications