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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2654 [new locus tag: SACOL_RS13900 ]
  • pan locus tag?: SAUPAN006361000
  • symbol: arcC2
  • pan gene symbol?: arcC
  • synonym:
  • product: carbamate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2654 [new locus tag: SACOL_RS13900 ]
  • symbol: arcC2
  • product: carbamate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2714930..2715871
  • length: 942
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAGAGAAAATTGTCATTGCATTAGGCGGTAATGCGATACAGACAACAGAAGCAACA
    GCTGAAGCACAACAAACAGCTATTAGATGTGCGATGCAAAACCTTAAACCTTTATTTGAT
    TCACCAGCGCGTATTGTGATTTCACATGGTAATGGTCCACAAATTGGAAGTTTATTAATC
    CAACAAGCTAAATCGAACAGTGACACAACGCCGGCAATGCCATTGGATACTTGTGGTGCA
    ATGTCACAGGGTATGATAGGCTATTGGTTGGAAACTGAAATCAATCGCATTTTAACTGAA
    ATGAATAGTGATAGAACTGTAGGCACAATCGTTACACGTGTGGAAGTAGATAAAGATGAT
    CCACGATTTGATAACCCAACTAAACCAATTGGTCCTTTTTATACGAAAGAAGAAGTTGAA
    GAATTACAAAAAGAACAGCCAGACTCAGTCTTTAAAGAAGATGCAGGACGTGGTTATAGA
    AAAGTAGTTGCGTCACCACTACCTCAATCTATACTAGAACACCAGTTAATTCGAACTTTA
    GCAGACGGTAAAAATATTGTCATTGCATGCGGTGGTGGCGGTATTCCAGTTATAAAAAAA
    GAAAATACCTATGAAGGTGTTGAAGCGGTTATAGATAAAGATTTTGCTAGTGAGAAATTA
    GCAACGCTGATTGAAGCAGATACCTTAATGATTCTTACGAATGTAGAAAATGTATTTATT
    AACTTTAATGAACCTAATCAACAACAAATCGATGATATTGATGTAGCAACACTGAAAAAA
    TACGCGGCACAAGGTAAGTTTGTGGAAGGATCGATGTTGCCAAAAATAGAAGCTGCGATA
    CGATTTGTTGAAAGTGGGGAAAACAAAAAAGTTATCATTACCAATTTAGAGCAGGCATAC
    GAAGCTTTGATTGGTAATAAAGGTACACACATTCACATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    942

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2654 [new locus tag: SACOL_RS13900 ]
  • symbol: ArcC2
  • description: carbamate kinase
  • length: 313
  • theoretical pI: 4.68382
  • theoretical MW: 34328.9
  • GRAVY: -0.269649

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.2?  ExPASy
    Carbamate kinase ATP + NH3 + CO2 = ADP + carbamoyl phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines carbamate kinase (TIGR00746; EC 2.7.2.2; HMM-score: 392.4)
    and 4 more
    Metabolism Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 49.8)
    Metabolism Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 25.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 16.7)
    Metabolism Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 11.7)
  • TheSEED  :
    • Carbamate kinase (EC 2.7.2.2)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Carbamate kinase (EC 2.7.2.2)
    and 2 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Carbamate kinase (EC 2.7.2.2)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Polyamine Metabolism  Carbamate kinase (EC 2.7.2.2)
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 93.8)
    and 1 more
    P-loop_NTPase (CL0023) SKI; Shikimate kinase (PF01202; HMM-score: 18.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.220753
    • TAT(Tat/SPI): 0.020079
    • LIPO(Sec/SPII): 0.011207
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPARIVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMNSDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYRKVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASEKLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKIEAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SACOL2657(arcA)arginine deiminase  [2] (data from MRSA252)
    SACOL1513(hup)DNA-binding protein HU  [2] (data from MRSA252)
    SACOL1477(ilvA1)threonine dehydratase  [2] (data from MRSA252)
    SACOL1745(pyk)pyruvate kinase  [2] (data from MRSA252)
    SACOL0584(rplA)50S ribosomal protein L1  [2] (data from MRSA252)
    SACOL2224(rplF)50S ribosomal protein L6  [2] (data from MRSA252)
    SACOL0585(rplJ)50S ribosomal protein L10  [2] (data from MRSA252)
    SACOL1257(rplS)50S ribosomal protein L19  [2] (data from MRSA252)
    SACOL2234(rplV)50S ribosomal protein L22  [2] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SACOL2206(rpsI)30S ribosomal protein S9  [2] (data from MRSA252)
    SACOL2214(rpsK)30S ribosomal protein S11  [2] (data from MRSA252)
    SACOL1448(sucB)dihydrolipoamide succinyltransferase  [2] (data from MRSA252)
    SACOL0731LysR family transcriptional regulator  [2] (data from MRSA252)
    SACOL0944NADH dehydrogenase  [2] (data from MRSA252)
    SACOL1759universal stress protein  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, ArcR* (activation) regulon, ArgR/AhrC (repression) regulon, CcpA regulon
    Rex*(TF)important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    ArcR*(TF)important in Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    ArgR/AhrC(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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