PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS00245 [old locus tag: SACOL0046 ]
• pan locus tag^?: SAUPAN000509000
• symbol: SACOL_RS00245
• pan gene symbol^?: —
• synonym:
• product: Zn-dependent hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS00245 [old locus tag: SACOL0046 ]
• symbol: SACOL_RS00245
• product: Zn-dependent hydrolase
• replicon: chromosome
• strand: -
• coordinates: 53428..54756
• length: 1329
• essential: unknown

⊟Accession numbers

• Location: NC_002951 (53428..54756) NCBI
• BioCyc: SACOL_RS00245 BioCyc
• MicrobesOnline: see SACOL0046

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGTTTTTTAAACAATTTTATGATAAACATTTATCTCAAGCATCTTATTTAATCGGTTGT
  CAAAAAACTGGAGAAGCCATGATTATTGATCCTATTCGTGACTTATCTTCATATATTCGA
  GTTGCTGATGAAGAAGGTTTAACCATTACTCATGCAGCTGAAACACATATACATGCAGAT
  TTTGCTTCAGGAATTAGAGATGTTGCTATAAAGTTAAATGCTAATATTTATGTATCGGGT
  GAAAGTGATGACACATTAGGTTATAAAAATATGCCTAACCACACTCATTTTGTTCAACAT
  AATGACGATATTTATGTAGGAAATATAAAATTAAAAGTGCTTCATACACCGGGTCACACG
  CCAGAAAGTATAAGTTTTTTACTTACTGACGAAGGTGCTGGAGCACAAGTTCCAATGGGA
  CTATTCAGTGGTGATTTTATTTTTGTAGGAGATATCGGTAGACCTGATTTACTAGAAAAA
  GCGGTTAAAGTAGAAGGATCATCTGAAATAGGCGCTAAACAAATGTTTAAATCGATTGAA
  AGTATTAAAGACTTGCCAGATTACATTCAAATTTGGCCTGGCCATGGCGCTGGTAGTCCT
  TGTGGTAAATCTTTAGGTGCTATTCCAACATCTACTCTTGGCTATGAAAAACAAACGAAC
  TGGGCTTTTTCTGAAAATAACGAAGCTACCTTTATCGATAAACTCATTTCTGACCAACCT
  GCACCACCACATCATTTTGCACAAATGAAAAAAATTAATCAATTCGGTATGAATTTATAT
  CAACCTTATACGGTTTATCCAGCTACAAATACAAACAGATTAACTTTTGATCTCCGCAGT
  AAGGAGGCTTATCATGGTGGACATATTGAAGGTACAATCAATATTCCATATGATAAAAAT
  TTCATCAATCAAATTGGCTGGTATCTAAACTATGATCAAGAAATTAACTTGATTGGAGAC
  TATCACCTTGTTTCAAAAGCAACACACACCTTACAACTCATTGGATACGATGATATTGCT
  GGATATCAATTACCGCAATCTAAGATTCAAACACGTTCCATTCATAGTGAAGACATTACA
  GGTAACGAATCACATATATTAGATGTACGTAATGATAATGAATGGAATAATGGCCACTTA
  TCTCAAGCGGTTCATGTACCACACGGCAAACTTTTAGAAACAGATTTACCTTTCAATAAA
  AACGATGTTATTTATGTACACTGTCAGTCTGGCATTAGAAGTTCGATAGCTATTGGTATT
  TTAGAACACAAAGGTTATCACAACATTATTAATGTAAATGAAGGTTATAAAGATATACAA
  CTTTCTTAA
  60
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  1329
  

⊟Protein

• 3R2U
  PDB

Protein Data Bank: 3R2U

⊟General

• locus tag: SACOL_RS00245 [old locus tag: SACOL0046 ]
• symbol: SACOL_RS00245
• description: Zn-dependent hydrolase
• length: 442
• theoretical pI: 5.97736
• theoretical MW: 49410
• GRAVY: -0.387104

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Detoxification hydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 74.5)
  and 3 more
  phage shock operon rhodanese PspE (TIGR02981; EC 2.8.1.1; HMM-score: 37)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 22)
  thiazole biosynthesis domain (TIGR04271; HMM-score: 20.9)
• TheSEED: see SACOL0046
• PFAM:
  Phosphatase (CL0031) Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 83)
  and 1 more
  Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 38.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9301
  • Cytoplasmic Membrane Score: 0.0311
  • Cell wall & surface Score: 0.0047
  • Extracellular Score: 0.0341
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008274
  • TAT(Tat/SPI): 0.001074
  • LIPO(Sec/SPII): 0.072689
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446387609 NCBI
• RefSeq: WP_000465464 NCBI
• UniProt: see SACOL0046

⊟Protein sequence

• MFFKQFYDKHLSQASYLIGCQKTGEAMIIDPIRDLSSYIRVADEEGLTITHAAETHIHADFASGIRDVAIKLNANIYVSGESDDTLGYKNMPNHTHFVQHNDDIYVGNIKLKVLHTPGHTPESISFLLTDEGAGAQVPMGLFSGDFIFVGDIGRPDLLEKAVKVEGSSEIGAKQMFKSIESIKDLPDYIQIWPGHGAGSPCGKSLGAIPTSTLGYEKQTNWAFSENNEATFIDKLISDQPAPPHHFAQMKKINQFGMNLYQPYTVYPATNTNRLTFDLRSKEAYHGGHIEGTINIPYDKNFINQIGWYLNYDQEINLIGDYHLVSKATHTLQLIGYDDIAGYQLPQSKIQTRSIHSEDITGNESHILDVRNDNEWNNGHLSQAVHVPHGKLLETDLPFNKNDVIYVHCQSGIRSSIAIGILEHKGYHNIINVNEGYKDIQLS

⊟Experimental data

• experimentally validated:
• protein localization: see SACOL0046
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications