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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS00440 [old locus tag: SACOL0088 ]
  • pan locus tag?: SAUPAN000899000
  • symbol: SACOL_RS00440
  • pan gene symbol?: nptA
  • synonym:
  • product: Na/Pi cotransporter

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS00440 [old locus tag: SACOL0088 ]
  • symbol: SACOL_RS00440
  • product: Na/Pi cotransporter
  • replicon: chromosome
  • strand: -
  • coordinates: 98200..99861
  • length: 1662
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (98200..99861) NCBI
  • BioCyc: SACOL_RS00440 BioCyc
  • MicrobesOnline: see SACOL0088

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCGGTTACAGAAGTCATTTTCTCCTTTTTAGGTGGTTTAGGTATTTTCCTTTACGGC
    TTAAAAATCATGGGAGACGGGCTTCAAGCATCAGCAGGAGACAGGCTACGAGATATTTTA
    AACAAATTTACATCAAATCCAGTATTAGGTGTTATTGCAGGTATCGTTGTAACTATTTTA
    ATACAAAGTAGTTCAGGTACGACAGTTATCACAATCGGACTGGTAACAGCTGGATTTATG
    ACATTGAAACAAGCCATTGGAGTGATAATGGGTGCTAATATCGGAACAACGGTAACTGCA
    TTTATTATCGGTATAGATTTAGGCGAATATGCAATGCCAATTTTAGCATTAGGTGCATTC
    TTAATCTTTTTCTTTAAACGCTCTAAAATCAATAACATTGGCCGCATACTATTCGGTTTC
    GGTTCACTATTCTTCGGTCTAGAATTTATGGGTGATGCCGTTAAACCTTTAGCATCATTA
    GATGGATTTAAGCAATTAATGCTTGATATGTCTACAAATCCAATACTCGCTGTCATTGTC
    GGCGCAGGGTTAACAGCACTAGTTCAAAGTTCAAGTGCGACGATTGGTATTTTACAAGAA
    TTTTATCAACAAGATTTAATTAGCTTAAACGCAGCAATCCCTGTGTTACTAGGCGATAAC
    ATTGGTACCACGATTACAGCTATCTTAGCTAGTTTAGCCGGCTCAATCGCTGCAAAACGT
    GCGGCGCTTGTACACGTCATCTTTAACTTAATCGGGGTAATTATCTTCACAATTTTCTTG
    CCAGTTGTGATTCATTTGATTAGTTTGTTACAAGATTTATGGCACTTAAAACCAGCGATG
    ACGATTGCAGTATCACATGGTATCTTCAACATAACAAATACTTTGATTCAATTACCATTT
    GTAGCAGGTTTAGCATGGATTGTTACAAAGCTTGTCCCAGGTAAAGATATTGCTGATGAC
    TATAAACCTCAGCACTTAAACAAAGATCTTGTTTATCACGCACCTGGTGTTGCATTACAA
    GAAACTCAAAAAGAATTACAAAATGTGGGTCAGATTGTCTTATCAATGTTTGAAGACATT
    CGCGAAATTACAAAAGACGATAAAAAATTGATCAAAAAGCTTGAACAAAAGCATCAAGCT
    GTTGAAACAATCAATGATAGCATTCGAAATTATTTAGTTAGAATTTCTACAAAAGCCATT
    ACGAAGGCAGACGTTGAGCGTTTAGCAGTTATGTTTGATGTCAATCGCTCTATTTTAAAA
    GTAGCAGAGCTAACAGAAGAGTATGTCGCTCAATTAAAACGCCAACATGATGAAGATATT
    CGCATTACAGAAGATGCACAACGCGGTATGGATAAATTATTCAACCATGTTGCTGAGTCA
    TTTGATAAAGCCATCGACATGTTAGATGTTTATGACAAAACGAAAAAAGATGAAATTGTA
    GAACGTAGTAGAGAATCATTTAATATTGAACATAAACTACGCAAAGGTCATATTAAACGC
    CTTAATCGTGGTGAATGTACAACAAAAGGCGGATTACTATATATCGATATGATTGGTGTT
    CTTGAACGTATCGGTTATCATTCACGAAATGTTTCTGAAGCACTTGTTGGCCTTAACGAT
    GATGTACCTACAGATGAAGAAATTGCAACAACTGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS00440 [old locus tag: SACOL0088 ]
  • symbol: SACOL_RS00440
  • description: Na/Pi cotransporter
  • length: 553
  • theoretical pI: 6.38787
  • theoretical MW: 60647.5
  • GRAVY: 0.333454

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds Na/Pi-cotransporter II-related protein (TIGR00704; HMM-score: 250.1)
    and 3 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds sodium-dependent inorganic phosphate (Pi) transporter (TIGR01013; HMM-score: 121.5)
    Metabolism Transport and binding proteins Anions phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 34.8)
    Signal transduction Regulatory functions Other phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 34.8)
  • TheSEED: see SACOL0088
  • PFAM:
    no clan defined Na_Pi_cotrans; Na+/Pi-cotransporter (PF02690; HMM-score: 232)
    and 7 more
    PhoU (CL0297) PhoU; PhoU domain (PF01895; HMM-score: 58.7)
    no clan defined ApoO; Apolipoprotein O (PF09769; HMM-score: 13)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 12.7)
    no clan defined IBP39; Initiator binding protein 39 kDa (PF11422; HMM-score: 12.4)
    FAD-oxidase_C (CL0277) Cytokin-bind; Cytokinin dehydrogenase 1, FAD and cytokinin binding (PF09265; HMM-score: 12)
    Phage_TACs (CL0567) Phage_TAC_19; Phage tail chaperone protein (PF23857; HMM-score: 11.4)
    NTF2 (CL0051) DUF7309; Domain of unknown function (DUF7309) (PF23988; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.9995
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0004
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.07588
    • TAT(Tat/SPI): 0.006252
    • LIPO(Sec/SPII): 0.006966
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFTSNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFIIGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASLDGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLGDNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHLKPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHAPGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYLVRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGMDKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTTKGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0088
  • protein localization: see SACOL0088
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

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