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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS00445 [old locus tag: SACOL0089 ]
  • pan locus tag?: SAUPAN000902000
  • symbol: SACOL_RS00445
  • pan gene symbol?: —
  • synonym:
  • product: oleate hydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS00445 [old locus tag: SACOL0089 ]
  • symbol: SACOL_RS00445
  • product: oleate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 100187..101962
  • length: 1776
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (100187..101962) NCBI
  • BioCyc: SACOL_RS00445 BioCyc
  • MicrobesOnline: see SACOL0089

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTATTACAGTTATGGAAATTATGAAGCATTTGCGCGCCCTAAAAAACCTGAAAATGTA
    GAAAACAAATCCGCTTACTTAATCGGATCTGGTCTAGCTTCACTTGCTGCAGCTTGTTTT
    TTAATAAGGGATGGTCAAATGGAAGGTTCGAAGATTCATATTTTAGAAGAGTTACCTAAA
    GCAGGTGGTAGTCTTGATGGTGAAAATATGCCTTTAAAAGGCTATGTTGTCCGCGGTGGT
    CGTGAAATGGAGAACCACTTTGAATGTTTGTGGGACTTATTCAGATCTATCCCTTCATTA
    GAAATCGATAACGCGTCTGTATTAGATGAGTTCTATTGGCTAAACAAAGAAGACCCTAAC
    TATTCTCGCTGTCGTGTTATTGAGAAACAGGGTCAACGTTTAGTCACAGACGGAGACTTC
    ACTTTGACTAAAACGGCGATTAAAGAAATTTTAGATTTATGCTTAACGAATGAAGAAGAT
    TTAGATGATGTCAAAATAACAGATGTATTTTCCGATGACTTCTTTAATTCAAACTTTTGG
    ATTTACTGGAAAACGATGTTTGCATTTGAACCGTGGCATTCTGCAATGGAAATGCGTCGC
    TATCTAATGCGATTCGTTCATCATATTAGTGGTCTCGCAGACTTTTCAGCTTTAAAATTC
    ACTAAATATAATCAATATGAATCTTTAGTATTACCTATGGTTGAATATTTAAAATCGCAT
    GGGGTTCAATTTGAATACGATGTAAAAGTCGAAGATATTAAAATAGATGTTACGACAAGT
    CAAAAAATTGCCCGAGAAATATTAATTGACCGTAATGGTAATGCAGAATCTATTAAACTG
    ACTATAAACGATCTTGTCTTTGTGACAAACGGTAGTATTACAGAAAGCTCTACTTATGGT
    GATAATGATACACCAGCGCCACCAACTGACGAATTAGGTGGTAGTTGGACACTATGGAAA
    AATTTAGCGCGACAAAGTCCTGAATTTGGTAATCCTGATAAGTTTTGCCAAAATATTCCT
    AAAAAAAGTTGGTTTGTTTCAGCAACTTCTACAACAAACAATAAAGAGATTATCGATACA
    ATAGAAAGTATTTGTAAACGTGACCCACTTGCAGGCAAAACAGTTACAGGCGGTATTATT
    ACAATCAATGATTCTGCATGGCAAATGAGTTTTACAATCAATCGTCAGCAACAGTTTAAA
    GACCAACCTGAAAATGAAATATCTACATGGATTTATGCCTTATATTCAGATGTAAACGGC
    GATTATATTAAAAAGCCAATTACAGAATGTAGTGGTAATGAAATATGCCAAGAATGGCTG
    TATCACTTAGGTGTATCAACTGACAAAATTGAAGACTTAGCAAAACATGCATCTAATACG
    ATTCCTGTTTATATGCCATATATCACATCTTATTTCATGACGCGTGCTATCGGCGACAGA
    CCTTTAGTCGTCCCGCATCAATCTCAGAACTTAGCATTTATTGGTAACTTTGCAGAAACA
    GAGCGAGACACTGTATTTACAACAGAATATTCGGTTCGTACTGCCATGGAAGCTGTTTAT
    CAATTACTAAATATAGATCGTGGTATTCCAGAAGTCATCAATAGTCCATTTGATCTTCGC
    GTCTTAATGGATGCCATATACGAACTGAATGACCACCAAGATTTGCGTGAGATTACTAAA
    GATTCGAAAATGCAAAAACTCGCATTAGCAGGATTCCTTAAAAAGATAAAAGGTACGTAC
    ATTGAGTCATTATTAAAAGAACACAAATTGTTATAA
    60
    120
    180
    240
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    360
    420
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    540
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    720
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    840
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    960
    1020
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    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1776

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS00445 [old locus tag: SACOL0089 ]
  • symbol: SACOL_RS00445
  • description: oleate hydratase
  • length: 591
  • theoretical pI: 4.75677
  • theoretical MW: 67648.2
  • GRAVY: -0.371235

⊟Function[edit | edit source]

  • TIGRFAM:
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 15.7)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 14.8)
  • TheSEED: see SACOL0089
  • PFAM:
    NADP_Rossmann (CL0063) MCRA; MCRA family (PF06100; HMM-score: 746.4)
    and 2 more
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 25)
    P-loop_NTPase (CL0023) AAA_15; AAA ATPase domain (PF13175; HMM-score: 15)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.031802
    • TAT(Tat/SPI): 0.005369
    • LIPO(Sec/SPII): 0.189365
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRDGQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEIDNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEEDLDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSALKFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAESIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDKFCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFTINRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKIEDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTTEYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQKLALAGFLKKIKGTYIESLLKEHKLL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL0089
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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