From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS00630 [old locus tag: SACOL0125 ]
  • pan locus tag?: SAUPAN000954000
  • symbol: SACOL_RS00630
  • pan gene symbol?: phnE1
  • synonym:
  • product: phosphonate ABC transporter, permease protein PhnE

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS00630 [old locus tag: SACOL0125 ]
  • symbol: SACOL_RS00630
  • product: phosphonate ABC transporter, permease protein PhnE
  • replicon: chromosome
  • strand: -
  • coordinates: 140420..141235
  • length: 816
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (140420..141235) NCBI
  • BioCyc: SACOL_RS00630 BioCyc
  • MicrobesOnline: see SACOL0125

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACACAGGAAATAGCAAAATATAATGTTCACACAAAAGCACACAAACGAAAATTGATT
    AAAAGATGGCTTATTGCAATTGTCGTCTTAGCTATTATCATCTGGGCATTTGCAGGTGTA
    CCAAGTTTAGAACTTAAAAGTAAATCATTAGAAATCTTAAAATCCATATTCAGCGGATTA
    TTCCATCCTGATATCAGCTATATCTATATACCAGATGGCGAAGACTTATTACGTGGTTTA
    CTTGAAACCTTTGCGATAGCCGTTGTAGGTACTTTCATCGCCGCAATTATCTGTATTCCA
    TTAGCATTTCTAGGTGCAAATAATATGGTAAAGCTACGCCCAGTTTCAGGTGTTAGCAAA
    TTTATTTTAAGTGTTATACGTGTCTTCCCAGAAATTGTAATGGCACTTATATTTATCAAA
    GCTGTTGGCCCAGGTTCATTTTCAGGTGTATTAGCTTTAGGTATCCATTCCGTAGGTATG
    CTTGGGAAACTTTTAGCTGAAGATATTGAAGGTCTAGATTTCAGTGCTGTAGAATCATTA
    AAGGCCAGTGGTGCGAATAAGATTAAAACACTCGTATTTGCAGTCATACCACAAATTATG
    CCTGCCTTTCTATCACTCATACTTTATCGCTTTGAACTAAACTTACGTTCAGCTTCTATA
    CTGGGGCTAATTGGGGCTGGTGGTATCGGGACACCACTCATATTTGCCATTCAAACACGT
    TCTTGGGACCGTGTAGGTATTATATTAATCGGTTTAGTACTAATGGTCGCAATTGTCGAT
    TTAATTTCCGGTTCAATCCGAAAACGTATTGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    816

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS00630 [old locus tag: SACOL0125 ]
  • symbol: SACOL_RS00630
  • description: phosphonate ABC transporter, permease protein PhnE
  • length: 271
  • theoretical pI: 10.4719
  • theoretical MW: 29286.2
  • GRAVY: 0.926568

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 267.8)
    and 6 more
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 24.7)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 21.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 19.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 14.5)
    Metabolism Transport and binding proteins Other energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 14.5)
    ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 9.9)
  • TheSEED: see SACOL0125
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 46.7)
    and 3 more
    no clan defined DUF4179; Domain of unknown function (DUF4179) (PF13786; HMM-score: 9.4)
    DUF5345; Family of unknown function (DUF5345) (PF17280; HMM-score: 9.2)
    SNARE; SNARE domain (PF05739; HMM-score: 8.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.236472
    • TAT(Tat/SPI): 0.00327
    • LIPO(Sec/SPII): 0.013786
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLELKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLAFLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGMLGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSASILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS00630&oldid=98654"