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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS02295 [old locus tag: SACOL0454 ]
- pan locus tag?: SAUPAN001985000
- symbol: SACOL_RS02295
- pan gene symbol?: tcyP
- synonym:
- product: L-cystine transporter
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS02295 [old locus tag: SACOL0454 ]
- symbol: SACOL_RS02295
- product: L-cystine transporter
- replicon: chromosome
- strand: -
- coordinates: 455229..456617
- length: 1389
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1381ATGAATGCTTTTCTTACACTGATTAATATTATTGTATTGGTGATTTTCATCGTAATATTG
CACATGATGGCTCGCAAACATATATCTTTTGCGAAACGTGTGTTTACAGCTTTAGGGATT
GGTATAGTATTCGGTGTGTTATTGCATCTTATTTATGGCACACATTCGAATGTTATTACA
TCAACAAGTGACTGGTTCAACATTGTCGGACAAGGTTACGTTGCATTGTTGCAAATGATT
GTTATGCCTTTAATTTTCATTTCTATTGTAGCTGCATTCACTAAAATTCAAATCGGAGAA
AAGTTTGCTAAAATTGGTAGTCTCATTTTTATCTTTTTAATTGGCACTGTCACCATTGCT
GCGATTGTTGGTGTTGTGTATGCCTTGGTGTTTGGTTTAGATGCATCAACAATTAACCTT
GGTAACGCTGAACAAGCACGTGGAAGCGAAATTGCTAAACAAGCGAAAGATTTAACAGCA
CATACGTTACCACAACAAATTTTGGAATTATTGCCTAAAAATCCATTCTTAGATTTCACT
GGACAACGTGCAACTTCCACGATTGCTGTCGTGATATTTGCTTCGTTTATAGGATTTGCT
TATTTACGCGTAGCACGCAAACAACCTGATCATGGTGAATTGCTTAAGCGTGCGATTGAC
GCTATCTATTCACTAGTAATGGCGATTGTGACGTTCGTCTTACGTTTAACGCCATACGGT
GTATTAGCAATTATGGCCAACACATTATCGACAAGTGACTTTGGCGCCATTTGGACTTTA
GGAAAATTCTTAATCGCATCGTACGCTGCATTAATTACGATGTATATCATTCACTTAATT
ATTTTAAGCCTGTTAGGTATTAGCCCAATTCGCTATGTTAAAAAGACTTTGGAAGTATTA
ATCTTTGCATTCACATCACGCTCAAGCGCGGGTGCCTTACCATTGAATGTACAAACACAA
ACAAGACGTCTAGGCGTGCCAGAAGGTATTGCCAATTTCGCTGCGACATTTGGATTGTCG
ATTGGACAAAATGGTTGTGCAGGCATTTACCCTGCCATGCTTGCAATTATGGTCGCACCC
GTCGCTAATGTAGAAATTGATTTACAATTTATTGTAACGTTAATCGCTGTCGTGATTATT
AGTTCATTCGGCGTTGCTGGCGTCGGTGGCGGGGCTACATTTGCATCCATATTGGTGTTG
TCAACGTTAAACTTGCCAGTCGCTTTAGCCGGCGTGCTCATCTCCGTTGAACCATTGATT
GACATGGGTAGAACAGCATTAAACGTGAACGATTCAATGCTAGCTGGTACAGGTACCGCC
AAGTTAACTAAACATTGGGATAAAGATACTTTCGATTCAAATGATAATGCTGCATTAACT
TCACACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS02295 [old locus tag: SACOL0454 ]
- symbol: SACOL_RS02295
- description: L-cystine transporter
- length: 462
- theoretical pI: 9.71226
- theoretical MW: 49412.3
- GRAVY: 0.87316
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: see SACOL0454
- PFAM: no clan defined SDF; Sodium:dicarboxylate symporter family (PF00375; HMM-score: 323.2)and 1 moreDUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 13.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 11
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.018907
- TAT(Tat/SPI): 0.000519
- LIPO(Sec/SPII): 0.002635
- predicted transmembrane helices (TMHMM): 9
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVFGVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKFAKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTAHTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRAIDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYIIHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFAATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGATFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDTFDSNDNAALTSH
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY, CymR* see SACOL0454
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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