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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0454 [new locus tag: SACOL_RS02295 ]
  • pan locus tag?: SAUPAN001985000
  • symbol: SACOL0454
  • pan gene symbol?: tcyP
  • synonym:
  • product: sodium:dicarboxylate symporter family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0454 [new locus tag: SACOL_RS02295 ]
  • symbol: SACOL0454
  • product: sodium:dicarboxylate symporter family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 455229..456617
  • length: 1389
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAATGCTTTTCTTACACTGATTAATATTATTGTATTGGTGATTTTCATCGTAATATTG
    CACATGATGGCTCGCAAACATATATCTTTTGCGAAACGTGTGTTTACAGCTTTAGGGATT
    GGTATAGTATTCGGTGTGTTATTGCATCTTATTTATGGCACACATTCGAATGTTATTACA
    TCAACAAGTGACTGGTTCAACATTGTCGGACAAGGTTACGTTGCATTGTTGCAAATGATT
    GTTATGCCTTTAATTTTCATTTCTATTGTAGCTGCATTCACTAAAATTCAAATCGGAGAA
    AAGTTTGCTAAAATTGGTAGTCTCATTTTTATCTTTTTAATTGGCACTGTCACCATTGCT
    GCGATTGTTGGTGTTGTGTATGCCTTGGTGTTTGGTTTAGATGCATCAACAATTAACCTT
    GGTAACGCTGAACAAGCACGTGGAAGCGAAATTGCTAAACAAGCGAAAGATTTAACAGCA
    CATACGTTACCACAACAAATTTTGGAATTATTGCCTAAAAATCCATTCTTAGATTTCACT
    GGACAACGTGCAACTTCCACGATTGCTGTCGTGATATTTGCTTCGTTTATAGGATTTGCT
    TATTTACGCGTAGCACGCAAACAACCTGATCATGGTGAATTGCTTAAGCGTGCGATTGAC
    GCTATCTATTCACTAGTAATGGCGATTGTGACGTTCGTCTTACGTTTAACGCCATACGGT
    GTATTAGCAATTATGGCCAACACATTATCGACAAGTGACTTTGGCGCCATTTGGACTTTA
    GGAAAATTCTTAATCGCATCGTACGCTGCATTAATTACGATGTATATCATTCACTTAATT
    ATTTTAAGCCTGTTAGGTATTAGCCCAATTCGCTATGTTAAAAAGACTTTGGAAGTATTA
    ATCTTTGCATTCACATCACGCTCAAGCGCGGGTGCCTTACCATTGAATGTACAAACACAA
    ACAAGACGTCTAGGCGTGCCAGAAGGTATTGCCAATTTCGCTGCGACATTTGGATTGTCG
    ATTGGACAAAATGGTTGTGCAGGCATTTACCCTGCCATGCTTGCAATTATGGTCGCACCC
    GTCGCTAATGTAGAAATTGATTTACAATTTATTGTAACGTTAATCGCTGTCGTGATTATT
    AGTTCATTCGGCGTTGCTGGCGTCGGTGGCGGGGCTACATTTGCATCCATATTGGTGTTG
    TCAACGTTAAACTTGCCAGTCGCTTTAGCCGGCGTGCTCATCTCCGTTGAACCATTGATT
    GACATGGGTAGAACAGCATTAAACGTGAACGATTCAATGCTAGCTGGTACAGGTACCGCC
    AAGTTAACTAAACATTGGGATAAAGATACTTTCGATTCAAATGATAATGCTGCATTAACT
    TCACACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1389

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0454 [new locus tag: SACOL_RS02295 ]
  • symbol: SACOL0454
  • description: sodium:dicarboxylate symporter family protein
  • length: 462
  • theoretical pI: 9.71226
  • theoretical MW: 49412.3
  • GRAVY: 0.87316

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • L-cystine uptake protein TcyP
    Sulfur Metabolism Organic sulfur assimilation L-Cystine Uptake and Metabolism  L-cystine uptake protein TcyP
  • PFAM:
    no clan defined SDF; Sodium:dicarboxylate symporter family (PF00375; HMM-score: 323.2)
    and 1 more
    DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018907
    • TAT(Tat/SPI): 0.000519
    • LIPO(Sec/SPII): 0.002635
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVFGVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKFAKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTAHTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRAIDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYIIHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFAATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGATFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDTFDSNDNAALTSH

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CymR* (repression) regulon, CodY (repression) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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