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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03095 [old locus tag: SACOL0597 ]
  • pan locus tag?: SAUPAN002323000
  • symbol: SACOL_RS03095
  • pan gene symbol?: hchA
  • synonym:
  • product: Molecular chaperone Hsp31 and glyoxalase 3

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03095 [old locus tag: SACOL0597 ]
  • symbol: SACOL_RS03095
  • product: Molecular chaperone Hsp31 and glyoxalase 3
  • replicon: chromosome
  • strand: +
  • coordinates: 622423..623301
  • length: 879
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (622423..623301) NCBI
  • BioCyc: SACOL_RS03095 BioCyc
  • MicrobesOnline: see SACOL0597

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCACAAGATGTAAATGAATTAAGTAAGCAACCAACGCCAGATAAAGCAGAAGATAAC
    GCATTTTTCCCATCACCATATTCCCTTAGTCAATATACAGCACCTAAAACAGATTTTGAT
    GGTGTTGAACACAAAGGTGCCTATAAAGATGGTAAATGGAAAGTATTGATGATTGCTGCT
    GAAGAGCGATATGTATTATTGGAAAATGGAAAAATGTTCTCTACGGGTAATCATCCTGTT
    GAAATGTTATTACCTTTACATCATTTAATGGAAGCAGGTTTTGACGTTGATGTTGCGACA
    TTATCTGGTTATCCAGTTAAATTAGAATTATGGGCTATGCCAACTGAAGACGAGGCAGTT
    ATAAGTACTTATAATAAATTGAAAGAAAAATTAAAACAGCCAAAAAAATTAGCAGATGTG
    ATTAAAAATGAATTAGGACCTGATTCAGACTATTTATCTGTCTTTATCCCAGGCGGACAT
    GCTGCAGTTGTTGGTATTTCTGAAAGTGAGGACGTTCAACAAACATTAGATTGGGCATTA
    GACAATGACCGCTTTATAGTTACATTATGTCATGGACCAGCAGCACTACTTTCAGCAGGG
    CTTAACAGAGAAAAATCTCCATTAGAAGGATACTCTGTTTGTGTCTTCCCTGACTCATTA
    GATGAAGGTGCAAATATTGAAATAGGTTATTTACCTGGACGCTTGAAATGGTTAGTTGCT
    GATTTATTAACTAAACAAGGATTAAAAGTAGTTAACGACGATATGACAGGAAGAACGTTA
    AAAGATCGTAAATTATTAACAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTA
    GCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    879

⊟Protein[edit | edit source]

Protein Data Bank: 5XR2
Protein Data Bank: 5XR3

⊟General[edit | edit source]

  • locus tag: SACOL_RS03095 [old locus tag: SACOL0597 ]
  • symbol: SACOL_RS03095
  • description: Molecular chaperone Hsp31 and glyoxalase 3
  • length: 292
  • theoretical pI: 4.65565
  • theoretical MW: 32176.4
  • GRAVY: -0.307877

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.124?  ExPASy
    Protein deglycase An N(omega)-(1-hydroxy-2-oxopropyl)-[protein]-L-arginine + H2O = a [protein]-L-arginine + (R)-lactate An N6-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H2O = a [protein]-L-lysine + (R)-lactate An S-(1-hydroxy-2-oxopropyl)-[protein]-L-cysteine + H2O = a [protein]-L-cysteine + (R)-lactate
    EC 3.1.2.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 28.8)
    and 1 more
    Unknown function General DJ-1 family protein (TIGR01383; HMM-score: 17.5)
  • TheSEED: see SACOL0597
  • PFAM:
    Glutaminase_I (CL0014) DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 38.6)
    and 2 more
    ThiJ_like; ThiJ/PfpI family-like (PF17124; HMM-score: 20.6)
    RND_permease (CL0322) ACR_tran; AcrB/AcrD/AcrF family (PF00873; HMM-score: 9.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006928
    • TAT(Tat/SPI): 0.001034
    • LIPO(Sec/SPII): 0.001259
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEHKGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLSGYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGHAAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPDSLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNELGKLAVNEMLNAIQNK

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0597
  • protein localization: see SACOL0597
  • quantitative data / protein copy number per cell: see SACOL0597
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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