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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS03635 [old locus tag: SACOL0707 ]
- pan locus tag?: SAUPAN002528000
- symbol: SACOL_RS03635
- pan gene symbol?: dhaK
- synonym:
- product: dihydroxyacetone kinase subunit DhaK
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS03635 [old locus tag: SACOL0707 ]
- symbol: SACOL_RS03635
- product: dihydroxyacetone kinase subunit DhaK
- replicon: chromosome
- strand: +
- coordinates: 732685..733653
- length: 969
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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961ATGATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTG
TTAATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAG
AAAGAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCC
GGTTTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCT
ACACCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTA
GTTGTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAA
ATGGAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAA
GTACAACGTCGTGGTGTTGCAGGAACAGTGTTTGTTCATAAGCTTGCCGGTTATCTTGCT
GAAAAAGGTTATTCATTAACAGAGATAAAATCGCGTGTAGAAGCGTTGTTACCTGAAATT
AAAAGTATTGGTATGGCAATTGAGCCACCGCTTGTTCCAACTACTGGAAAATATGGCTTT
GATATTGAAGACGACAAAATGGAAATCGGTATTGGTATACATGGTGAAAAAGGTATTCAT
AGGGAAGAAGTAAAGGATATTGATCATATTGTTGGAACATTGTTAGACGAATTGTATAAA
GAAGTTACTGCCAATGATGTCATATTAATGGTAAATGGTATGGGTGGTACGCCGTTATCT
GAATTAAATATCGTAACTAAATATATTCAACAAAATTTAGCTGCAAGAACGGTTAATGTT
GCTAAATGGTTTGTTGGTGATTATATGACATCTTTAGACATGCAAGGTTTTTCTATAACT
ATCGTGCCTAATAAACCAGAATATTTGGAAGCATTTTTAGCACCAACAACAAGTCAATAC
TTTAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS03635 [old locus tag: SACOL0707 ]
- symbol: SACOL_RS03635
- description: dihydroxyacetone kinase subunit DhaK
- length: 322
- theoretical pI: 4.70202
- theoretical MW: 35018.3
- GRAVY: 0.0608696
⊟Function[edit | edit source]
- TIGRFAM: dihydroxyacetone kinase, DhaK subunit (TIGR02363; EC 2.7.1.-; HMM-score: 479.3)and 3 moredihydroxyacetone kinase (TIGR02361; EC 2.7.1.29; HMM-score: 311.7)probable dihydroxyacetone kinase DhaK1b subunit (TIGR02362; HMM-score: 263)dihydroxyacetone kinase, phosphotransfer subunit (TIGR02364; HMM-score: 14.2)
- TheSEED: see SACOL0707
- PFAM: EDD (CL0245) Dak1; Dak1 domain (PF02733; HMM-score: 384)and 2 moreEIIA-man; PTS system fructose IIA component (PF03610; HMM-score: 14.6)no clan defined DUF3139; Protein of unknown function (DUF3139) (PF11337; HMM-score: 12.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.011421
- TAT(Tat/SPI): 0.00143
- LIPO(Sec/SPII): 0.000659
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHGVAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVVKNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLAEKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKGIHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAARTVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CcpA see SACOL0707
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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