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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS03680 [old locus tag: SACOL0715 ]
- pan locus tag?: SAUPAN002537000
- symbol: SACOL_RS03680
- pan gene symbol?: graX
- synonym:
- product: NAD(P)-dependent oxidoreductase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS03680 [old locus tag: SACOL0715 ]
- symbol: SACOL_RS03680
- product: NAD(P)-dependent oxidoreductase
- replicon: chromosome
- strand: +
- coordinates: 739549..740472
- length: 924
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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901ATGAAACCTAAAGTTTTATTAGCAGGTGGAACAGGATATATTGGTAAGTATTTAAGTGAA
GTGATTGAAAATGATGCTGAACTTTTTGCTATATCAAAATATCCAGACAATAAAAAAACA
GATGATGTTGAAATGACTTGGATTCAGTGTGATATATTTCATTACGAACAGGTTGTTGCA
GCAATGAATCAAATAGATATTGCTGTATTCTTTATCGACCCAACAAAGAATTCTGCCAAA
ATAACACAATCATCAGCAAGAGATTTAACATTAATCGCAGCAGATAATTTTGGTCGAGCA
GCGGCTATCAATCAAGTAAAAAAAGTAATCTACATACCTGGGAGTCGTTATGATAATGAA
ACAATTGAACGCCTAGGTGCATATGGTACACCTGTAGAAACAACAAATTTAGTTTTTAAA
CGTTCTTTAGTTAATGTAGAATTACAAGTTTCAAAGTATGATGATGTTAGATCAACGATG
AAGGTAGTTTTACCAAAGGGATGGACATTAAAGAACGTTGTAAACCATTTTATTGCATGG
ATGGGTTACACTAAAGGAACTTTTGTGAAAACAGAAAAATCACATGATCAATTTAAGATA
TATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGA
ATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGG
AAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTAT
ATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATG
ATTCATGGCTTTGAAGTTGACTGCCGGATTAAAGATTTTCAAAGTCGATTAAAATCAGGA
GAAAATATGAAATATACTAAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS03680 [old locus tag: SACOL0715 ]
- symbol: SACOL_RS03680
- description: NAD(P)-dependent oxidoreductase
- length: 307
- theoretical pI: 9.62557
- theoretical MW: 35234.8
- GRAVY: -0.14886
⊟Function[edit | edit source]
- TIGRFAM: Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 13)
- TheSEED: see SACOL0715
- PFAM: NADP_Rossmann (CL0063) NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 23.5)Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 22.8)and 1 more3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 15.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.016804
- TAT(Tat/SPI): 0.00014
- LIPO(Sec/SPII): 0.001705
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVEMTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAAINQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTMKVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETADGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPMQKMMIHGFEVDCRIKDFQSRLKSGENMKYTK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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